FLP-mediated conditional loss of an essential gene to facilitate complementation assays Page: 34
View a full description of this thesis.
Extracted Text
The following text was automatically extracted from the image on this page using optical character recognition software:
Bglllmut F7518 EcoRI ()
pUC118-P450
7527bp
BamHI (4387)EcoRI BglII
I /P450i CD
P450 CDS
\ NotI (2483)
PspOMImut R2496
I
Ligati
POMI EcoRI/PspOMI
restriction 1
digestionpUC118-P450
7527bpBamHI(4387/
3amHI(4387)
EcoRI/NC
restrictio
EcoRI (1)BglipUC118-P450mut
P450 CDS
\ Notl (2483)
otI
)n digestionP450 CDS
BamHI
BamHIFigure 11: Schematic representation of steps involved in creating the pUC118-P450mut plasmid.
The undesirable NotI and introduced restriction sites PspOMI and BglII are represented in red.
The primer binding sites are indicated as green arrows.34
r . i
(1)
::..::
:::
Upcoming Pages
Here’s what’s next.
Search Inside
This thesis can be searched. Note: Results may vary based on the legibility of text within the document.
Tools / Downloads
Get a copy of this page or view the extracted text.
Citing and Sharing
Basic information for referencing this web page. We also provide extended guidance on usage rights, references, copying or embedding.
Reference the current page of this Thesis.
Ganesan, Savita. FLP-mediated conditional loss of an essential gene to facilitate complementation assays, thesis, December 2007; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc5180/m1/44/: accessed July 16, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .