FLP-mediated conditional loss of an essential gene to facilitate complementation assays Page: 19
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LB no aacCl 35 o Ori aadA EASESp-4 FLP nos RB
t
attB1
Figure 8: Second vector pCAM-Gent-TSpSUC2-EASE-FLP T-DNAdelivering the FLP
recombinase. aacCl: Gene encoding gentamycin resistance under control of the 35S promoter
and nos terminator, for selection of transgenic plants; 35Sp: Cauliflower mosaic virus
constitutive 35S promoter; lox: Cre recombinase recognition site; Ori: pBR322 bacterial origin
of replication; aadA: Gene encoding kanamycin resistance for selection of transformed bacteria;
TSpSUC2: Functional AtSUC2 cDNA under the control of tissue specific promoter and nos
terminator; Sbfl, KpnI, EcoRI: Cloning sites for cloning the TSpSUC2 cassette; EASE: Egg
Apparatus-Specific Enhancer driving the expression of FLP recombinase; 35Sp-45: Cauliflower
mosaic virus 35S promoter -45 region; FLP: Gene encoding the site-specific FLP recombinase;
aatBl: Gateway recombination site; LB: T-DNA left border; RB: T-DNA right border.19
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Ganesan, Savita. FLP-mediated conditional loss of an essential gene to facilitate complementation assays, thesis, December 2007; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc5180/m1/29/: accessed July 16, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .