Effects of Cigarette Smoke Condensates on Cultured Human Lymphocytes and Separation of Benzo-α-Pyrene Metabolites by High Pressure Liquid Chromatography Page: 2
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Ghanayem, Burhan, Effects of Cigarette Smoke Condensates on
Cultured Human Lymphocytes and Separation of Benzo-a-pyrene
Metabolites by High Pressure Liquid Chromatography. Master of
Science (Basic Health Sciences), August 1979, 82 pp., 16 figures,
1 table, bibliography, 91 titles.
Cigarette smoke condensates from all cigarettes tested were
found to be potent inducers of AHH enzyme in cultured human
lymphocytes and, with the exception of Kent Lights and Carlton
CSC's, all were found to be toxic under the experiment conditions.
Most of the AHH inducing activity was found in basic and neutral
fractions of the lAl standard cigarettes.
A radiometric assay of BP metabolites in cultured human
lymphocytes was developed in which we were able to separate the
primary metabolites and the secondary metabolites from the parent
compound (BP) by neutral alumnia HPLC. The primary metabolites
were further separated by a selective enzyme hydrolysis and/or
reverse phase HPLC.
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Ghanayem, Burhan I. Effects of Cigarette Smoke Condensates on Cultured Human Lymphocytes and Separation of Benzo-α-Pyrene Metabolites by High Pressure Liquid Chromatography, thesis, August 1979; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc503989/m1/2/: accessed July 18, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .