Mutation of Polaris, an Intraflagellar Transport Protein, Shortens Neuronal Cilia Metadata

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Title

  • Main Title Mutation of Polaris, an Intraflagellar Transport Protein, Shortens Neuronal Cilia

Creator

  • Author: Mahato, Deependra
    Creator Type: Personal

Contributor

  • Chair: Fuchs, Jannon L.
    Contributor Type: Personal
    Contributor Info: Major Professor
  • Chair: Schwark, Harris
    Contributor Type: Personal
    Contributor Info: Co-Major Professor
  • Committee Member: Gross, Guenter W.
    Contributor Type: Personal

Publisher

  • Name: University of North Texas
    Place of Publication: Denton, Texas

Date

  • Creation: 2005-08
  • Digitized: 2008-02-12

Language

  • English

Description

  • Content Description: Primary cilia are non-motile organelles having 9+0 microtubules that project from the basal body of the cell. While the main purpose of motile cilia in mammalian cells is to move fluid or mucus over the cell surface, the purpose of primary cilia has remained elusive for the most part. Primary cilia are shortened in the kidney tubules of Tg737orpk mice, which have polycystic kidney disease due to ciliary defects. The product of the Tg737 gene is polaris, which is directly involved in a microtubule-dependent transport process called intraflagellar transport (IFT). In order to determine the importance of polaris in the development of neuronal cilia, cilium length and numerical density of cilia were quantitatively assessed in six different brain regions on postnatal days 14 and 31 in Tg737orpk mutant and wildtype mice. Our results indicate that the polaris mutation leads to shortening of cilia as well as decreased percentage of ciliated neurons in all brain regions that were quantitatively assessed. Maintainance of cilia was especially affected in the ventromedial nucleus of the hypothalamus. Furthermore, the polaris mutation curtailed cilium length more severely on postnatal day 31 than postnatal day 14. These data suggests that even after ciliogenesis, intraflagellar transport is necessary in order to maintain neuronal cilia. Regional heterogeneity in the effect of this gene mutation on neuronal cilia suggests that the functions of some brain regions might be more compromised than others.

Subject

  • Library of Congress Subject Headings: Carrier proteins.
  • Library of Congress Subject Headings: Cilia and ciliary motion.
  • Library of Congress Subject Headings: Mice -- Nervous system.
  • Keyword: Polaris
  • Keyword: neuronal cilia
  • Keyword: Tg737orpk
  • Keyword: primary cilia
  • Keyword: intraflagellar transport
  • Keyword: IFT

Collection

  • Name: UNT Theses and Dissertations
    Code: UNTETD

Institution

  • Name: UNT Libraries
    Code: UNT

Rights

  • Rights Access: public
  • Rights License: copyright
  • Rights Holder: Mahato, Deependra
  • Rights Statement: Copyright is held by the author, unless otherwise noted. All rights reserved.

Resource Type

  • Thesis or Dissertation

Format

  • Text

Identifier

  • OCLC: 68182162
  • Archival Resource Key: ark:/67531/metadc4856

Degree

  • Degree Name: Master of Science
  • Degree Level: Master's
  • Degree Discipline: Biology
  • Academic Department: Department of Biological Sciences
  • Degree Grantor: University of North Texas

Note

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