Fungal Antigens and Fungal Disease: An Alkali-Soluble, Water Soluble Antigen from Coccidioides immitis and Coccidioidomycosis

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Diagnostic medical mycology has been slow to advance due to a lack of species specific antigens in organisms which cause serious diseases in man. Toward this end, an HPLC analysis was done of the following fungal antigens: histoplasmins HKC-43 and H-42, blastomycin KCB-26, an alkali-soluble, water soluble antigen from Blastomyces dermatitidis (b-ASWS), a coccidioidin prepared from a toluene lysate of the mycelial-arthroconidia phase of Coccidioides immitis, and an alkali-soluble, water-soluble antigen from Coccidioides immitis (c-ASWS). The HPLC survey included size-exclusion chromatography (SEC), ion exchange chromatography (HPIEC), and reversephase chromatography (RP). Resolution was poor with both SEC and HPIEC but was … continued below

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viii, 204 leaves : ill.

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Fleming, William H. (William Harold) December 1983.

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Diagnostic medical mycology has been slow to advance due to a lack of species specific antigens in organisms which cause serious diseases in man. Toward this end, an HPLC analysis was done of the following fungal antigens: histoplasmins HKC-43 and H-42, blastomycin KCB-26, an alkali-soluble, water soluble antigen from Blastomyces dermatitidis (b-ASWS), a coccidioidin prepared from a toluene lysate of the mycelial-arthroconidia phase of Coccidioides immitis, and an alkali-soluble, water-soluble antigen from Coccidioides immitis (c-ASWS). The HPLC survey included size-exclusion chromatography (SEC), ion exchange chromatography (HPIEC), and reversephase chromatography (RP). Resolution was poor with both SEC and HPIEC but was excellent with RP chromatography. The use of RP will allow sufficient separation for further antigenic and structural analysis.

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viii, 204 leaves : ill.

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Fleming, William H. (William Harold). Fungal Antigens and Fungal Disease: An Alkali-Soluble, Water Soluble Antigen from Coccidioides immitis and Coccidioidomycosis, dissertation, December 1983; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc331669/: accessed June 30, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .

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