Analysis of a Human Transfer RNA Gene Cluster and Characterization of the Transcription Unit and Two Processed Pseudogenes of Chimpanzee Triosephosphate Isomerase Page: 80
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80
Fig. 9. Comparison of cloned chimpanzee TPI sequences
with chimpanzee and human genomic DNAs by Southern blot
hybridization. The recombinant chimpanzee DNAs, harbored in
X-chpTPI-1, to|fchpTPI-A, and A,\j/chpTPI-B (0.2 \ig) , and
chimpanzee and human genomic DNAs (10 |Ag) were digested with
(A) PstI or (B) EcoRI. The restricted DNAs were
fractionated on 0.8% agarose gels, transferred to
positively-charged nylon membranes, and hybridized with 32p-
labeled DNA fragments derived from an upstream segment of
the human TPI cDNA clone pHTPI-5a (Maquat et al., 1985).
The sizes (in kilobases) of the fragments shown were
determined relative to standard markers generated by
digesting X DNA with Hindlll and pBR322 DNA with Hinfl.
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Craig, Leonard C. (Leonard Callaway). Analysis of a Human Transfer RNA Gene Cluster and Characterization of the Transcription Unit and Two Processed Pseudogenes of Chimpanzee Triosephosphate Isomerase, dissertation, August 1990; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc331579/m1/86/: accessed July 18, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .