Pleomorphism in Selected Genera of Algae Page: 29
vi, 79 leaves : ill.View a full description of this thesis.
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CHAPTER II
MATERIALS AND METHODS
For this research, pure cultures of Ankistrodesmus
falcatus (Chorda) Ralfs #188 and Tetraedron bitridens (Beck)
#120 were obtained from the culture collection of Indiana
University, Bloomington (20), and one unialgal culture of
Ankistrodesmus sp. was obtained from the culture collection
of North Texas State University, Denton. These cultures and
their subcultures were maintained on agar slants of soil-
water extract (12, 14, 11, 19) in a culture unit at 25° C.
with sixteen hours of light per twenty—four hour period.
Complete and deficient Bold1s Basal Media were prepared
following the procedure as outlined by Brown and Bold (2).
Six macroelement stock solutions were prepared by dissolving
the indicated weight of the following salts in 400 ml. of
distilled de-ionized water:
NaN03 10.0 gm.
KH2PO4 7.0 gm.
K2HPO4 3.0 gm.
MgS04-7H20 3.0 gm.
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Bruton, Barbara A. Pleomorphism in Selected Genera of Algae, thesis, June 1967; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc163903/m1/35/: accessed July 17, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .