Molecular mechanism by which cyclic amp regulates myocardial contractility Page: 46 of 153
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RESULTS
Sodium Dodecyl Sulfate - Polyacryl ami de Slab Gel Electrophoresis of
Cardiac Sarcopl asmi c Reti cul urn
Fig. 1 shows a 12% SDS-polyacryl ami de slab gel of cardiac
microsomes. The 22,000 dal ton protein, phosphol amban, identified
by Tada jet _a[. (1975), appears as a distinct band on the slab gel.
In this gel system, phosphol amban runs with a molecular weight of
23,500 when compared to molecular weight standards. For
consistency, phosphol amban will be referred to as a 22,000 dal ton
protein in this paper. A 5,000 dal ton protein, which is also
phosphoryl ated by cycl ic AFP-dependent protein kinase, appears as a
diffuse band just below the major low molecular weight protein
band. The 6,000 dal ton protein appears in Coomassie blue stained
gels only when a high concentration of methanol is present in the
stain. In addition to these two proteins, the slab gel shows the
presence of many proteins and especially higher molecular weight
proteins not previously identified by disc gels (Tada _et _al., 1975).
Phosphorylation of a 6,000 dalton and a 22,000 dalton protein by
cyclic AlP-dependent Protein Kinase
Slab gels have the distinction over disc gels in that many
different conditions of phosphorylation can be compared directly by
doing an autoradiogram of the slab gel. As can be seen in Fig. 2,
maximal phosphorylation of the 22,000 dalton and 6,000 dalton
protein occur when both cyclic AMP and protein kinase were included
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Bidlack, J. M. Molecular mechanism by which cyclic amp regulates myocardial contractility, report, January 1, 1979; United States. (https://digital.library.unt.edu/ark:/67531/metadc1085147/m1/46/: accessed July 17, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.