Molecular mechanism by which cyclic amp regulates myocardial contractility Page: 37 of 153
Pages: 142View a full description of this report.
Extracted Text
The following text was automatically extracted from the image on this page using optical character recognition software:
METHODS
Isolation of Cardiac Microsomes
Cardiac microsomes were prepared from canine heart ventricle
according to Harigaya and Schwartz (1969) with the following
modifications. Hearts were removed from dogs anesthesized with
Nembutal. The hearts were placed in ice-cold saline. Fat and
connective tissue were removed and the ventricle was cut in small
pieces. The heart muscle was homogenized in 10 mM NaHCO^ in a
Waring blendor. The suspension was centrifuged at 8,700 x g for 20
min. The supernatant was strained through 4 layers of cheesecloth
and spun at 10,000 x g for 20 min. The supernatant was again
strained through cheesecloth, followed by centrifugation at 37,000
x g for 30 min. The pellet was suspended in 20 mM tris-maleate,
0.6 M KC1, pH 6.8. This suspension was centrifuged at 100,000 x g
for 20 min to remove the solubilized actomyosin. The pellet from
this centrifugation was suspended in 0.25 M sucrose. Protein
concentration was determined by the method of Lowry et_ aj_. (1951)
with bovine serum albumin as the standard.
Upcoming Pages
Here’s what’s next.
Search Inside
This report can be searched. Note: Results may vary based on the legibility of text within the document.
Tools / Downloads
Get a copy of this page or view the extracted text.
Citing and Sharing
Basic information for referencing this web page. We also provide extended guidance on usage rights, references, copying or embedding.
Reference the current page of this Report.
Bidlack, J. M. Molecular mechanism by which cyclic amp regulates myocardial contractility, report, January 1, 1979; United States. (https://digital.library.unt.edu/ark:/67531/metadc1085147/m1/37/: accessed July 17, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.