Studies on mammalian chromosomes. Final report. [Satellite DNA sequences in rat cells] Page: 4 of 13
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ABSTRACT
1. The locations of satellite DNA sequences have been studied
in Kangaroo rat cell lines by in situ hybridization. Satellite HS-p is located
in the kinetochores of 32 of the 35 chromosomes. Satellite HD is located
in the kinetochores of the other three chromosomes and in the short arms of
most of the 35 chromosomes.
These satellite DNAs are replicated in 4 fixed order in the last half
of S period.
Measurements of the DNA content of 7 species of kangaroo rats
show that there is no precise relationship between amount of satellite DNA
and genome size. In cultured cells, the amount of satellite DNA can be
altered by treatment with bromodeoxyuridlne.
2. The nuclear envelope of mammalian cells has been shown to
have no role in the Initiation and continua Lion of DNA synthesis .
3. a) Isolabeling of chromosomes has been acounted for by
sister chromatid exchanges coupled with image spread of autoradiographs.
b) Sister chromatid exchanges occur preferentially at the
kinetochore. Small differences in the frequency of exchanges occur at
different positions in chromosome arms.
4. a and p polymerase have been shown by a new cell frac-
tionation technique to be nuclear enzymes. Thymidine kinase is a cytoplasmic
enzyme.
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Prescott, D.M. Studies on mammalian chromosomes. Final report. [Satellite DNA sequences in rat cells], report, September 1, 1975; Boulder, Colorado. (https://digital.library.unt.edu/ark:/67531/metadc1055557/m1/4/: accessed July 16, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.