UNT Libraries - 8 Matching Results

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Developing a Generalizable Two-Input Genetic AND Logic Gate in Arabidopsis thaliana for Multi-Signal Processing

Description: With effective engineering using synthetic biology approaches, plant-based platforms could conceivably be designed to minimize the production costs and wastes of high-value products such as medicines, biofuels, and chemical feedstocks that would otherwise be uneconomical. Additionally, modern agricultural crops could be engineered to be more productive, resilient, or restorative in different or rapidly changing environments and climates. To achieve these complex goals, information-processing ge… more
Date: December 2022
Creator: Anderson, Charles Edgar
Item Type: Refine your search to only Thesis or Dissertation

Developing Informatics Tools and Methods Utilizing Whole Genome Sequencing and Transcription Data to Aid Gene Discovery in Medicago truncatula

Description: Research into the mechanism of symbiotic nitrogen fixation between legumes and rhizobia involves a complex interaction between the organisms, and many genes involved in this remain either uncharacterized or undiscovered. Using forward genetics, mutant plant lines are screened to find new genes without reliance on software-based gene prediction. A large population of Tnt1-mutagenized Medicago truncatula lines is used for this purpose. Herein, the aid of tools like whole genome sequencing (WGS) i… more
This item is restricted from view until January 1, 2025.
Date: December 2022
Creator: Troiani, Taylor
Item Type: Refine your search to only Thesis or Dissertation

Identification and Characterization of Two Putative Sulfate Transporters Essential for Symbiotic Nitrogen Fixation in Medicago truncatula

Description: The process of symbiotic nitrogen fixation (SNF) in legume root nodules requires the channeling and exchange of nutrients within and between the host plant cells and between the plant cells and their resident rhizobia. Using a forward genetics approach in the Medicago truncatula Tnt1 mutant population followed by whole genome sequencing, two putative sulfate transporter genes, MtSULTR3;5 and MtSULTR3;4b, were identified. To support the hypothesis that the defective putative sulfate transporter … more
This item is restricted from view until January 1, 2025.
Date: December 2022
Creator: Pradhan, Rajashree
Item Type: Refine your search to only Thesis or Dissertation

Investigation of Gene Functions in the Cyanotrophic Bacterium Pseudomonas fluorescens NCIMB 11764

Description: Pseudomonas fluorescens NCIMB 11764 (Pf11764) is one of a group of bacteria known as cyanotrophs that exhibit the unique ability to grow on toxic cyanide as the sole nitrogen source. This ability has previously been genetically linked to a conserved cluster of seven genes (Nit1C), the signature gene (nitC) coding for a nitrilase enzyme. Nitrilases convert nitriles to ammonia and a carboxylic acid. Still, for the Pf11764 NitC enzyme (Nit11764), no in vivo substrate has been identified, and the… more
This item is restricted from view until June 1, 2027.
Date: May 2022
Creator: Gullapalli, Jaya Swetha
Item Type: Refine your search to only Thesis or Dissertation

Medicago truncatula NPF1.7: Structure-Function Assessment and Potential as a Phytohormone Transporter

Description: In Medicago truncatula, the MtNPF1.7 transporter has been shown to be essential for root morphology and nodulation development. The allelic MtNPF1.7 mutants, Mtnip-1 (A497V), Mtnip-3 (E171K), and Mtlatd (W341STOP), show altered lateral root growth and compromised legume-rhizobium symbiosis. To assess the role of a series of distinct amino acids in the transporter's function, in silico structural predictions were combined with in planta complementation of the severely defective Mtnip-1 mutant pl… more
Date: December 2022
Creator: Yu, Yao Chuan
Item Type: Refine your search to only Thesis or Dissertation

Role of DEFECTIVE IN SYSTEMIC DEFENSE INDUCED BY ABIETANE DITERPENOID 1 (DSA1), a Putative O-Fucosyltransferase, in Plant Systemic Acquired Resistance (SAR)

Description: Dehydroabietinal (DA), an abietane diterpenoid, was previously demonstrated to be a potent activator of systemic acquired resistance (SAR). DA also promotes flowering time in Arabidopsis thaliana by repressing expression of the flowering repressor FLOWERING LOCUS C (FLC) while simultaneously upregulating expression of FLOWERING LOCUS D (FLD), FLOWERING LOCUS VE (FVE) and RELATIVE OF EARLY FLOWERING 6 (REF6), a set of flowering time promoters. To further understand the mechanism underlying signa… more
Date: May 2022
Creator: Mohanty, Devasantosh
Item Type: Refine your search to only Thesis or Dissertation
open access

Seeing in the Light: Using Expansion Microscopy to Achieve Super-Resolution in Transmitted Light

Description: Light microscopy is inherently limited in resolution by properties of light such as diffraction and interference to 170-250 nm. Expansion microscopy is a quickly-developing method which achieves super-resolution by using a swellable hydrogel to physically expand biological samples themselves, rather than depending on the properties of fluorophores. This thesis demonstrates that expansion microscopy is a feasible means for achieving super-resolution in transmitted light microscopy modes. Though … more
Date: December 2022
Creator: Migliore, Julia R.
Item Type: Refine your search to only Thesis or Dissertation

A Sensitive and Robust Machine Learning-Based Framework for Deciphering Antimicrobial Resistance

Description: Antibiotics have transformed modern medicine in manifold ways. However, the misuse and over-consumption of antibiotics or antimicrobials have led to the rise in antimicrobial resistance (AMR). Unfortunately, robust tools or techniques for the detection of potential loci responsible for AMR before it happens are lacking. The emergence of resistance even when a strain lacks known AMR genes has puzzled researchers for a long time. Clearly, there is a critical need for the development of novel app… more
This item is restricted from view until September 1, 2024.
Date: August 2022
Creator: Sunuwar, Janak
Item Type: Refine your search to only Thesis or Dissertation
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