FLP-mediated conditional loss of an essential gene to facilitate complementation assays Page: VII
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1. Cycling conditions for touchdown PCR used for plant genotyping .................................24
2. Cycling conditions for the first PCR amplification of Bar gene from pGPTV-BAR........29
3. Cycling conditions for the second mutagenic PCR amplification of Bar gene ................30
4. Cycling conditions for the final round of PCR amplification of Bar gene.......................30
5. PCR conditions for P450 gene cassette amplification with desired mutations ................33
6. Annealing conditions used for synthetic fragment construction.......................................40
7. PCR conditions for amplification of synthetic fragment ..................................................40
8. Cycling conditions for the first and second PCR amplification of FLP gene ..................44
9. PCR conditions for the final amplification of FLP gene ..................................................44
10. Cycling conditions for the first and second round of PCR amplification of the aacCl gene
............................................................................................................................................46
11. Cycling conditions for molecular analysis of excision by PCR .......................................53vii
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Ganesan, Savita. FLP-mediated conditional loss of an essential gene to facilitate complementation assays, thesis, December 2007; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc5180/m1/8/: accessed May 7, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .