Development and Use of a Switchgrass (Panicum Virgatum L.) Transformation Pipeline by the Bioenergy Science Center to Evaluate Plants for Reduced Cell Wall Recalcitrance
Description
This article provides information on the organization and outcomes of the BioEnergy Science Center Transformation Pipeline, and supplies useful information when developing coordinated, large-scale, multi-institutional reverse genetic pipelines to improve crop traits.
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22 p.
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Nelson, Richard S.; Stewart, C. Neal, Jr.; Gou, Jiqing; Holladay, Susan; Gallego-Giraldo, Lina; Flanagan, Amy et al. December 22, 2017.
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Authors
- Nelson, Richard S. Noble Research Institute, LLC; Oak Ridge National Laboratory
- Stewart, C. Neal, Jr. University of Tennessee, Knoxville; Oak Ridge National Laboratory
- Gou, Jiqing Noble Research Institute, LLC; Oak Ridge National Laboratory
- Holladay, Susan Oak Ridge National Laboratory
- Gallego-Giraldo, Lina Oak Ridge National Laboratory; University of North Texas
- Flanagan, Amy Noble Research Institute, LLC; Oak Ridge National Laboratory
- Mann, David G. J. University of Tennessee, Knoxville; Oak Ridge National Laboratory
- Hisano, Hiroshi Noble Research Institute, LLC; Oak Ridge National Laboratory
- Wuddineh, Wegi A. University of Tennessee, Knoxville; Oak Ridge National Laboratory
- Poovaiah, Charleson R. University of Tennessee, Knoxville; Oak Ridge National Laboratory
- Srivastava, Avinash C. Noble Research Institute, LLC; Oak Ridge National Laboratory
- Biswal, Ajaya K. Oak Ridge National Laboratory; University of Georgia
- Shen, Hui Oak Ridge National Laboratory; University of North Texas
- Escamilla-Treviño, Luis Oak Ridge National Laboratory; University of North Texas
- Yang, Jiading Noble Research Institute, LLC; Oak Ridge National Laboratory
- Hardin, C. Frank Noble Research Institute, LLC; Oak Ridge National Laboratory
- Nandakumar, Rangaraj Noble Research Institute, LLC; Oak Ridge National Laboratory
- Fu, Chunxiang Noble Research Institute, LLC; Oak Ridge National Laboratory
- Fu, Chunxiang Noble Research Institute, LLC; Oak Ridge National Laboratory
- Zhang, Jiyi Noble Research Institute, LLC; Oak Ridge National Laboratory
- Xiao, Xirong Noble Research Institute, LLC; Oak Ridge National Laboratory
- Percifield, Ryan Oak Ridge National Laboratory; University of Georgia
- Chen, Fang Oak Ridge National Laboratory; University of North Texas
- Bennetzen, Jeffery L. Oak Ridge National Laboratory; University of Georgia
- Udvardi, Michael K. Noble Research Institute, LLC; Oak Ridge National Laboratory
- Mazarei, Mitra University of Tennessee, Knoxville; Oak Ridge National Laboratory
- Dixon, R. A. Oak Ridge National Laboratory; University of North Texas
- Wang, Z.-Y. (Zeng-Yu), 1963- Noble Research Institute, LLC; Oak Ridge National Laboratory
- Tang, Yuhong Noble Research Institute, LLC; Oak Ridge National Laboratory
- Mohnen, Debra Oak Ridge National Laboratory; University of Georgia
- Davison, Brian H. (Brian Henry), 1957- Oak Ridge National Laboratory
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BioMed Central Ltd.
Place of Publication: London, United Kingdom
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UNT College of Science
The College of Science provides students with the high-demand skills and knowledge to succeed as researchers and professionals. The College includes four departments: Biology, Chemistry, Math, and Physics, and is also home to a number of interdisciplinary programs, centers, institutes, intercollegiate programs, labs, and services.
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- Department: Biological Sciences
- Department: BioDiscovery Institute
Description
This article provides information on the organization and outcomes of the BioEnergy Science Center Transformation Pipeline, and supplies useful information when developing coordinated, large-scale, multi-institutional reverse genetic pipelines to improve crop traits.
Physical Description
22 p.
Notes
Abstract
Background: The mission of the BioEnergy Science Center (BESC) was to enable efficient lignocellulosic-based biofuel
production. One BESC goal was to decrease poplar and switchgrass biomass recalcitrance to biofuel conversion
while not affecting plant growth. A transformation pipeline (TP), to express transgenes or transgene fragments (constructs)
in these feedstocks with the goal of understanding and decreasing recalcitrance, was considered essential for
this goal. Centralized data storage for access by BESC members and later the public also was essential.
Results: A BESC committee was established to codify procedures to evaluate and accept genes into the TP. A
laboratory information management system (LIMS) was organized to catalog constructs, plant lines and results from
their analyses. One hundred twenty-eight constructs were accepted into the TP for expression in switchgrass in the
first 5 years of BESC. Here we provide information on 53 of these constructs and the BESC TP process. Eleven of the
constructs could not be cloned into an expression vector for transformation. Of the remaining constructs, 22 modified
expression of the gene target. Transgenic lines representing some constructs displayed decreased recalcitrance in the
field and publications describing these results are tabulated here. Transcript levels of target genes and detailed wall
analyses from transgenic lines expressing six additional tabulated constructs aimed toward modifying expression of
genes associated with wall structure (xyloglucan and lignin components) are provided. Altered expression of xyloglucan
endotransglucosylase/hydrolases did not modify lignin content in transgenic plants. Simultaneous silencing of two
hydroxycinnamoyl CoA:shikimate hydroxycinnamoyl transferases was necessary to decrease G and S lignin monomer
and total lignin contents, but this reduced plant growth.
Conclusions: A TP to produce plants with decreased recalcitrance and a LIMS for data compilation from these plants
were created. While many genes accepted into the TP resulted in transgenic switchgrass without modified lignin or
biomass content, a group of genes with potential to improve lignocellulosic biofuel yields was identified. Results from
transgenic lines targeting xyloglucan and lignin structure provide examples of the types of information available on
switchgrass lines produced within BESC. This report supplies useful information when developing coordinated, largescale,
multi-institutional reverse genetic pipelines to improve crop traits.
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Source
- Biotechnology for Biofuels, 10(309), BioMed Central, December 22, 2017, pp. 1-22
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- Digital Object Identifier: https://doi.org/10.1186/s13068-017-0991-x
- Archival Resource Key: ark:/67531/metadc1062056
Publication Information
- Publication Title: Biotechnology for Biofuels
- Volume: 10
- Article Identifier: 309
- Peer Reviewed: Yes
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- December 22, 2017
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- Jan. 23, 2018, 5:28 a.m.
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Nelson, Richard S.; Stewart, C. Neal, Jr.; Gou, Jiqing; Holladay, Susan; Gallego-Giraldo, Lina; Flanagan, Amy et al. Development and Use of a Switchgrass (Panicum Virgatum L.) Transformation Pipeline by the Bioenergy Science Center to Evaluate Plants for Reduced Cell Wall Recalcitrance, article, December 22, 2017; London, United Kingdom. (https://digital.library.unt.edu/ark:/67531/metadc1062056/: accessed May 1, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT College of Science.