Search Results

open access

The Water-Water Cycle Is Essential for Chloroplast Protection in the Absence of Stress

Description: Article showing that knockdown Arabidopsis plants with suppressed expression of the key water-water cycle enzyme, thylakoid-attached copper/zinc superoxide dismutase (KD-SOD), are suppressed in their growth and development. This article provides e genetic evidence for the importance of the water-water cycle in protecting the photosynthetic apparatus of higher plants from photooxidative damage.
Date: October 3, 2003
Creator: Rizhsky, Ludmila; Liang, Hongjian & Mittler, Ron
Partner: UNT College of Science
open access

Malonylation of Glucosylated N-Lauroylethanolamine: A New Pathway That Determines N-Acylethanolamine Metabolic Fate in Plants

Description: Article studying Malonylation of Glucosylated N-Lauroylethanolamine. Results indicate that glucosylation of NAE 12:0 by a yet to be determined glucosyltransferase and its subsequent malonylation by PMAT1 could represent a mechanism for modulating the biological activities of NAEs in plants.
Date: December 30, 2016
Creator: Khan, Bibi Rafeiza; Wherritt, Daniel J.; Huhman, David; Sumner, Lloyd W.; Chapman, Kent Dean & Blancaflor, Elison B.
Partner: UNT College of Science
open access

Isorhamnetin Inhibits Proliferation and Invasion and Induces Apoptosis through the Modulation of Peroxisome Proliferator-activated Receptor γ Activation Pathway in Gastric Cancer

Description: Article investigating the potential effects of isorhamnetin (IH), a 3′-O-methylated metabolite of quercetin on the peroxisome proliferator-activated receptor γ (PPAR-γ) signaling cascade using proteomics technology platform, GC cell lines, and xenograft mice model. The study y proposes a novel agent for gastric cancer treatment.
Date: November 2, 2012
Creator: Ramachandran, Lalitha; Manu, Kanjoormana Aryan; Shanmugam, Muthu K.; Li, Feng; Siveen, Kodappully Sivaraman; Vali, Shireen et al.
Partner: UNT College of Science
open access

Lipoxygenase-mediated Oxidation of Polyunsaturated N-Acylethanolamines in Arabidopsis

Description: Article hypothesizing that lipoxygenase (LOX) participated in the metabolism of PU-NAEs through the formation of NAE-oxylipins. The data suggests that Arabidopsis LOXs indeed compete with FAAH to metabolize PU-NAEs during seedling establishment. Identification of endogenous amide-conjugated oxylipins suggests potential significance of these metabolites in vivo, and FAAH mutants may offer opportunities to address this in the future.
Date: April 29, 2011
Creator: Kilaru, Aruna; Herrfurth, Cornelia; Keereetaweep, Jantana; Hornung, Ellen; Venables, Barney J.; Feussner, Ivo et al.
Partner: UNT College of Science
open access

Visualization of Lipid Droplet Composition by Direct Organelle Mass Spectrometry

Description: Article reporting an approach that combines the visualization of individual lipid droplets (LDs), microphase extraction of lipid components from droplets, and the direct identification of lipid composition by nanospray mass spectrometry, even to the level of a single LD. The triacylglycerol (TAG) composition of LDs from several plant sources (mature cotton (Gossypium hirsutum) embryos, roots of cotton seedlings, and Arabidopsis thaliana seeds and leaves) were examined by direct organelle mass spectrometry and revealed the heterogeneity of LDs derived from different plant tissue sources.
Date: February 4, 2011
Creator: Horn, Patrick J.; Ledbetter, Nicole R.; James, Christopher N.; Hoffman, William D.; Case, Charlene R.; Verbeck, Guido F. et al.
Partner: UNT College of Science
open access

Temperature-sensitive Post-translational Regulation of Plant Omega-3 Fatty-acid Desaturases Is Mediated by the Endoplasmic Reticulum-associated Degradation Pathway

Description: Article expressing two closely related plant FAD3 genes in yeast cells and found that their enzymes produced significantly different amounts of omega-3 fatty acids and that these differences correlated to differences in rates of protein turnover. The findings indicate that Fad3 protein abundance is regulated by a combination of cis-acting degradation signals and the ubiquitin-proteasome pathway and that modulation of Fad3 protein amounts in response to temperature may represent one mechanism of homeoviscous adaptation in plants.
Date: July 9, 2010
Creator: O'Quin, Jami B.; Bourassa, Linda; Zhang, Daiyuan; Shockey, Jay M.; Gidda, Satinder K.; Fosnot, Spencer et al.
Partner: UNT College of Science
open access

Unraveling Δ1-Pyrroline-5-Carboxylate-Proline Cycle in Plants by Uncoupled Expression of Proline Oxidation Enzymes

Description: Article showing that overexpression of MsProDH in tobacco and Arabidopsis or impairment of P5C oxidation in the Arabidopsis p5cdh mutant did not change the cellular Pro to P5C ratio under ambient and osmotic stress conditions, indicating that P5C excess was reduced to Pro in a mitochondrial-cytosolic cycle. Results show that normal oxidation of P5C to Glu by P5CDH is key to prevent P5C-Pro intensive cycling and avoid ROS production from electron run-off.
Date: September 25, 2009
Creator: Miller, Gad; Honig, Arik; Stein, Hanan; Suzuki, Nobuhiro; Mittler, Ron & Zilberstein, Aviah
Partner: UNT College of Science
open access

Mutations in Arabidopsis Fatty Acid Amide Hydrolase Reveal That Catalytic Activity Influences Growth but Not Sensitivity to Abscisic Acid or Pathogens

Description: Article showing that the enhanced growth and NAE tolerance of the AtFAAH overexpressing seedlings depends on the catalytic activity of AtFAAH, hypersensitivity to ABA and hypersusceptibility to nonhost pathogens are independent of its enzymatic activity. Findings suggest that the AtFAAH influences plant growth and interacts with ABA signaling and plant defense through distinctly different mechanisms.
Date: December 4, 2009
Creator: Kim, Sang-Chul; Kang, Li; Nagaraj, Satish; Blancaflor, Elison B.; Mysore, Kirankumar S. & Chapman, Kent D.
Partner: UNT College of Science
open access

The Transcriptional Co-activator MBF1c Is a Key Regulator of Thermotolerance in Arabidopsis thaliana

Description: Article reporting that the highly conserved transcriptional co-activator, MBF1c (multiprotein bridging factor 1c), is a key regulator of thermotolerance in Arabidopsis thaliana. Results provide evidence for the existence of a tightly coordinated heat stress-response network, involving trehalose-, SA-, and ethylene-signaling pathways, that is under the control of MBF1c.
Date: April 4, 2008
Creator: Suzuki, Nobuhiro; Bajad, Sunil; Shuman, Joel; Shulaev, Vladimir & Mittler, Ron
Partner: UNT College of Science
open access

Ascorbate Peroxidase 1 Plays a Key Role in the Response of Arabidopsis thaliana to Stress Combination

Description: Article conducting proteomic and metabolic analysis of Arabidopsis thaliana plants subjected to a combination of drought and heat stress. The authors identify 45 different proteins that specifically accumulated in Arabidopsis in response to the stress combination. The findings suggest that cytosolic APX1 plays a key role in the acclimation of plants to a combination of drought and heat stress.
Date: December 5, 2008
Creator: Koussevitzky, Shai; Suzuki, Nobuhiro; Huntington, Serena; Armijo, Leigh; Sha, Wei; Cortes, Diego F. et al.
Partner: UNT College of Science
open access

The EAR-motif of the Cys2/His2-type Zinc Finger Protein Zat7 Plays a Key Role in the Defense Response of Arabidopsis to Salinity Stress

Description: Article reporting that transgenic Arabidopsis plants constitutively expressing the Cys2/His2 zinc finger protein Zat7 have suppressed growth and are more tolerant to salinity stress. Findings demonstrate that the EAR-domain of Cys2/His2-type zinc finger proteins plays a key role in the defense response of Arabidopsis to abiotic stresses.
Date: March 23, 2007
Creator: Ciftci-Yilmaz, Sultan; Morsy, Mustafa R.; Song, Luhua; Coutu, Alicia; Krizek, Beth A.; Lewis, Michael W. et al.
Partner: UNT College of Science
open access

Function of Conserved Residues of Human Glutathione Synthetase: Implications for the ATP-Grasp Enzymes

Description: Article examining the mechanism of Glutathione synthetase, an enzyme that belongs to the glutathione synthetase ATP-binding domain-like superfamily. A variety of structural alignment methods were applied and four highly conserved residues of human glutathione synthetase (Glu-144, Asn-146, Lys-305, and Lys-364) were identified in the binding site. The function of these was studied by experimental and computational site-directed mutagenesis.
Date: May 21, 2004
Creator: Dinescu, Adriana; Cundari, Thomas R., 1964-; Bhansali, Vikas S.; Luo, Jia-Li & Anderson, Mary E.
Partner: UNT College of Science
open access

Phosphorylation of Myosin Light Chain Kinase by p21-activated Kinase PAK2

Description: Article reporting that PAK2, a member of the Rho family of GTPase-dependent kinases, regulates isometric tension development and myosin II RLC phosphorylation in saponin permeabilized endothelial monolayers. The results demonstrate that PAK2 can directly phosphorylate MLCK, inhibiting its activity and limiting the development of isometric tension.
Date: June 16, 2000
Creator: Goeckeler, Zoe M.; Masaracchia, Ruthann A.; Zeng, Qi; Chew, Teng-Leong; Gallagher, Patricia & Wysolmerski, Robert B.
Partner: UNT College of Science
open access

Activation of an S6/H4 Kinase (PAK 65) from Human Placenta by Intramolecular and Intermolecular Autophosphorylation

Description: Article proposing a model in which phosphorylation of sites 1 and 2 occurs sequentially. The model proposes that trypsin treatment of the inactive holoenzyme removes an inhibitory rac-binding domain which blocks MgATP access to the catalytic site. The pseudosubstrate domain at site 1 is autophosphorylated and subsequent bimolecular autophosphorylation at site 2 fully opens the catalytic site.
Date: September 8, 1995
Creator: Benner, Gretchen E.; Dennis, Patrick B. & Masaracchia, Ruthann A.
Partner: UNT College of Science
open access

Multisite phosphorylation of a synthetic peptide derived from the carboxyl terminus of the ribosomal protein S6

Description: Article synthesizing and testing the synthetic peptide AKRRRLSSLRASTSKSESSQK (56-21) which corresponds to the carboxyl-terminal 21 amino acids of human ribosomal protein S6 as a substrate for S6/H4 kinase purified from human placenta. The data suggests that multiple S6 kinases may be required to phosphorylate S6 at all five sites which are modified in vivo.
Date: January 5, 1991
Creator: Brandon, Stanley D. & Masaracchia, Ruthann A.
Partner: UNT College of Science
open access

Activation of an S6 Kinase From Human Placenta by Autophosphorylation

Description: Article describing a novel S6 kinase isolated from human placenta which autoactivates through autophosphorylation in vitro. The data supports the hypothesis that this previously uncharacterized S6 kinase belongs to a unique family of protein kinases which utilize autophosphorylation as part of their in vivo activation mechanism.
Date: September 15, 1993
Creator: Dennis, Patrick B. & Masaracchia, Ruthann A.
Partner: UNT College of Science
open access

Revision and Extension of a Generally Applicable Group-Additivity Method for the Calculation of the Standard Heat of Combustion and Formation of Organic Molecules

Description: Article presenting the calculation of the heats of combustion ΔH°c and formation ΔH°f of organic molecules at standard conditions using a commonly applicable computer algorithm based on the group-additivity method. This work is a continuation and extension of an earlier publication.
Date: October 10, 2021
Creator: Naef, Rudolf & Acree, William E. (William Eugene)
Partner: UNT College of Science
open access

Trypsin induces an aversive response in zebrafish by PAR2 activation in keratinocytes

Description: Article using zebrafish as a model and identifies that trypsin induces an aversive response in zebrafish larvae and adult zebrafish. Results show that the trypsin activates PAR2 on keratinocytes signaling the brain, and this pathway of trypsin-induced escape response will provide a unique communication mechanism in zebrafish.
Date: October 8, 2021
Creator: Alsrhani, Abdullah; Raman, Revathi & Jagadeeswaran, Pudur
Partner: UNT College of Science
open access

Beyond the Chicken: Alternative Avian Models for Developmental Physiological Research

Description: This article is a review examining the characteristics that make an animal model attractive for developmental research and explores opportunities presented by the embryo to adult continuum of alternative bird models including quail, ratites, songbirds, birds of prey, and corvids.
Date: October 21, 2021
Creator: Flores-Santin, Josele & Burggren, Warren W.
Partner: UNT College of Science
open access

Excited State Charge Separation in an Azobenzene-Bridged Perylenediimide Dimer – Effect of Photochemical Trans-Cis Isomerization

Description: Article demonstrating photoinduced charge transfer and separation events in a newly synthesized azobenzene-bridged perylenediimide-dimer (PDI-dimer). Results offer key insights on the role of the azobenzene bridge and the dimer geometry in governing the excited state charge transfer and separation in symmetrically linked PDI dimer.
Date: September 20, 2021
Creator: Zink-Lorre, Nathalie; Seetharaman, Sairaman; Gutiérrez-Moreno, David; Fernández-Lázaro, Fernando; Karr, Paul A. & D'Souza, Francis
Partner: UNT College of Science
open access

A Miniature Permanent Magnet Assembly with Localized and Uniform Field with an Application to Optical Pumping of Helium

Description: Article using simulations and experimental measurements to design, build, and test a compact and uniform magnetic field source and then apply it to the optical pumping of atomic helium.
Date: September 24, 2021
Creator: Cameron, Garnet; Cuevas, Jonathan; Pound, Jeffrey Jr. & Shiner, David
Partner: UNT College of Science
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