Sensitive Microtiter Assays for NAD, NADP and Protein Quantification in Human Lymphocytes
Description:
Intracellular levels of NAD are of renewed interest in clinical and basic science research due to the new discovery of enzymes which utilize NAD as a substrate. Microtiter assays for the determination of NAD, NADP and protein were developed as modifications of previously published methods. The resulting assays are simple, cost effective and sensitive. An improved method of isolating lymphocytes was also developed. The resultant procedure requires one hour and removes greater than 99.9% of the p…
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Date:
May 1990
Creator:
Johnson, James, 1964-
Partner:
UNT Libraries