A Solution of the Distributional Error Problem in Cytophotometry
Description:
Use of that portion of the characteristic curve of photographic film in which transmission is linear with log exposure eliminates the distributional error and thus makes possible the assessment of total mass of an inhomogeneously distributed cell consistent without scanning or measuring the cell's projected area. Measurement consists of obtaining the difference in output of a photocell receiving the total light transmitted by a photomicrograph of the cell, and a photomicrograph of the microscope field illumination against which the cell was photographed. A method of making such measurements is given, and its basis is described.
Date:
December 19, 1961
Creator:
Adams, Lawrence R. & Sondhaus, Charles A.
Item Type:
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Partner:
UNT Libraries Government Documents Department