Construction of a broad-host-range Anderson promoter series and particulate methane monoxygenase promoter variants expand the methanotroph genetic toolbox
Description:
This article determined the copy number of three broad-host-range plasmids in Methylococcus capsulatus Bath and Methylosinus trichosporium OB3b, representing phylogenetically diverse Gammaproteobacterial and Alphaproteobacterial methanotrophs, respectively. The authors mutagenized the native M. Capsulatus particulate methane monooxygenase promoter and identified variants with activity that expand the activity range of synthetic, constitutive promotors functional not only in M. capsulatus, but a…
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Date:
February 28, 2024
Creator:
Bhat, Etash H.; Henard, Jessica M.; Lee, Spencer A.; McHalffey, Dustin; Ravulapati, Mahith S.; Rogers, Elle V. et al.
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UNT College of Science