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Two-Dimensional Gel Electrophoresis of in Vivo and in Vitro Synthesized Proteins, Antigenic Proteins, and Cross-Reactive Antigens in Treponema Pallidum Subsp. Pallidum Nichols Strain and Treponema Phagedenis Biotype Reiter

Description: Two-dimensional electrophoretic protein profiles of in vivo and in vitro propagated T.pallidum subsps. pallidum Nichols strain were analyzed and compared. This comparative analysis revealed two in vitro synthesized, cytoplasmic cylinder-associated polypeptides with molecular masses 29.5 and 34.7 kDa, pI 5.62, and one in vitro "lost" polypeptide with molecular mass 34.7 kDa, pI 5.34. integral membrane proteins of in vitro and in vivo propagated T. pallidum was identified by phase partitioning wi… more
Date: May 1989
Creator: Sayahtaheri, Sousan
Partner: UNT Libraries
open access

Characterization of the Outer Membrane of Treponema Pallidum Subsp. Pallidum by Binding Studies Using Antibodies, Complement, and Host Serum Proteins

Description: The major goal of this study was to achieve sustained cultivation of virulent T. pallidum in vitro. The putatuive binding of host proteins to the outer membrane (OM) of intact, virulent T. pallidum subsp. pallidum has been investigated. A major breakthrough was the development of a filtration assay, usinglow protein-binding membrane filters, for the measurement of substances bound to or incorporated into th eOM of T. pallidum. This avoided the conventional manipulations which can damage the fra… more
Date: December 1989
Creator: Chang, Po-Hsun
Partner: UNT Libraries
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