Developments and Applications of Electrophoresis and Small Molecule Laser Desorption Ionization Mass Spectrometry
Description:
Ultra-sensitive native fluorescence detection of proteins with miniaturized one- and two-dimensional polyacrylamide gel electrophoresis was achieved with laser side-entry excitation, which provides both high excitation power and low background level. The detection limit for R-phycoerythrin protein spots in 1-D SDS-PAGE was as low as 15 fg, which corresponds to 40 thousand molecules only. The average detection limit of six standard native proteins was 5 pg per band and the dynamic range spanned …
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Date:
December 1, 2007
Creator:
Zhang, Hui
Partner:
UNT Libraries Government Documents Department