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Evidence for the Interaction of GTP with Rat Liver Glyoxalase II

Description: Glyoxalase 11, the second enzyme of the glyoxalase system, hydrolyzes S-D-lactoylglutathione (SLG) to regenerate glutathione (GSH) and liberate free D-lactate. It was found that GTP binds with Gil from rat liver and inhibits Gil activity. Preincubation experiments showed that the binding is relatively tight, since more than 15 minutes are required to release GTP from the complex following dilution. Inhibition kinetics studies indicate that GTP is a "partially competitive inhibitor"; Thus, it would appear that the binding sites for substrate (SLG) and inhibitor (GTP) are different, but spatially close. Glyoxalase 11 binds to a GTP affinity medium, and with polyacrylamide gel electrophoresis, Gil has a higher relative mobility when GTP is present (ATP has no effect). The functional consequences of GTP binding with a specific site on Gil are still unclear. It is speculated that Gil may interact with tubulin by serving as a dissociable GTP carrier, delivering GTP to the tubulinGTP binding site, and thus facilitating tubulin polymerization.
Date: December 1991
Creator: Yuan, Win-Jae
Partner: UNT Libraries

Toward a Critique of the Message Construct in Communication

Description: The idea of an inherent structure of meaning in human communication is identified as the message construct. Traces of the construct in textbook models of the basic communication process and in popular magazine images of communication are examined. The argument is raised that objectifying the message has resulted in a paradigm which focuses disproportionately on explicit, representational, and instrumental aspects of communication. An alternative conception is proposed which would take into account implicit, constitutive, and generative aspects.
Date: August 1991
Creator: Wilson, John K. (John Kenneth)
Partner: UNT Libraries

The Writers and Writing of Computer User Documentation: A Social Perspective

Description: This thesis studies the writing of computer user documentation from a social perspective by examining the process of creating computer documentation and the role of documentation writers in the work place. This study consisted of in-depth interviews and observations of four writers of computer user documentation.
Date: August 1991
Creator: Webb, Sheree C.
Partner: UNT Libraries

Factor Analysis of Health Concerns in the Chronic Back Pain Patient-MMPI2

Description: The purpose of this study was to analyze the factor structure of items pertaining to health on the Minnesota Multiphasic Personality Inventory (MMPI2) for chronic back pain patients in comparison to a control group. The results may be used as groundwork for developing an MMPI2 subscale to describe this population. The groups differed in the sequence of the resulting factors and the percentage of variance accounted for by each factor. The factors extracted when evaluating the control group were titled in order: Poor Physical Health, Digestive Difficulties, Equilibrium, Depression/Malaise, and Multiple Somatic Complaints. Resulting factors for the pain group were: Depression/Malaise, Digestive Difficulties, Multiple Somatic Complaints, Headaches/Dizziness, and Neurological Reaction/Poor Physical Health.
Date: August 1991
Creator: McGee-Hall, Joanne M. (Joanne Moore)
Partner: UNT Libraries

Sleep Patterns and Chronic Pain

Description: Sleep, emotions and pain are intimately connected, physiologically, by their location and utilization of the same brain centers and neurotransmitters. Sleep disturbances have been clinically observed in chronic pain populations; yet, no treatment program has formally addressed this aspect of patient care. It is hypothesized that a pain population (PN) will differ significantly from a non-injured workforce (WF) when reviewing quantitative and qualitative sleep data. This study strongly supports that sleep disturbances and socioeconomic decrements exist in chronic pain patients. Forty-seven variables were surveyed and 13 were found to show significant differences between the groups and seven were found to discriminate between the PN and WF groups at less than the .0001 level. A discriminant analysis was performed to determine the smallest model which could efficiently classify cases, according to successive root variables. The major discriminators are pain levels, medication, amount of sleep obtained and number of awakenings.
Date: August 1991
Creator: Kellen, Rebecca Margaret
Partner: UNT Libraries

Synthesis and Kinetic Mechanism Study of Phosphonopeptide as a Dead-End Inhibitor of cAMP-Dependent Protein Kinase

Description: DL-2-Amino-4-phosphonobutyric acid, an isostere of phosphoserine, was incorporated into the heptapeptide sequence, Leu-Arg-Arg-Ala-(DL-2-amino-4-phosphonobutyric acid)-Leu-Gly, for kinetic mechanistic studies of the cAMP-dependent protein kinase. To block the phosphono hydroxyl groups, methyl, ethyl and 4nitrobenzyl esters were studied as possible protecting groups. The phosphono diethyl ester of the N-Fmoc-protected amino acid was utilized in the synthesis of the heptapeptide. Two configurational forms of the protected peptide were obtained and were separated by C18-reverse phase HPLC. Characterization of the two isomeric forms was accomplished by 3 1P NMR, 1H NMR, 13C% NMR and amino acid analysis. The protecting groups of the isomeric phsophonopeptides were removed by HBr/AcOH and purified by cation exchange HPLC. Both phosphonopeptides were found to be inhibitors of the cAMP-dependent protein kinase, having Ki values of 0.6 mM (peptide A) and 1.9 mM (peptide B).
Date: December 1991
Creator: Yang, Chunhua
Partner: UNT Libraries

Personality Profiles of Hospitality Students: A Comparison of These Traits to Those Preferred by the Hospitality Industry

Description: One problem facing the hospitality industry today is turnover. Management turnover rates of 50 and 75 percent continue to plaque all segments of the industry. Personality type theory holds that people are happier in environments that are compatible with their personalities. This study examines 229 undergraduate students enrolled in hospitality education at the University of North Texas. The Myers Briggs Type Indicator was administered to these students to determine their predominant personality types, and to compare these types to those desired by hospitality industry professionals for success within the industry. Variables such as gender, work experience, and classification were also examined in comparison to student personality types.
Date: December 1991
Creator: Martin, Lynda (Lynda Jean)
Partner: UNT Libraries

D. H. Lawrence: Misogyny as Ideology in His Later Works of Fiction and Nonfiction

Description: Critics continue to debate Lawrence's attitude toward women: Some say Lawrence is a misogynist, some say he is an egalitarian, and others say he is ambivalent toward women. If Lawrence's works are divided into two chronological periods, before and after 1918, these differences of opinions begin to dissolve. Lawrence is fair in his treatment of women in the earlier works; however, in his later works Lawrence restricts women to what he calls the sensual realm, the realm of feelings and emotions. In addition, Lawrence denounces all women who assert individuality and self-responsibility. In the later works, Lawrence's ideology restricts the role of women and presents male supremacy as the natural and necessary order for human existence.
Date: August 1991
Creator: Hester, Vicki M. (Vicki Martin)
Partner: UNT Libraries

Pre-Steady State Kinetics of the NAD-Malic Enzyme from Ascaris suum in the Direction of Oxidative Decarboxylation of L-Malate

Description: Stopped-flow experiments in which the NAD-malic enzyme was preincubated with different reactants at near saturating substrate concentrations suggest a slow isomerization of the E:NAD:Mg complex. The lag is eliminated by preincubation with Mg˙² and malate suggesting that the formation of E:Mg:Malate either bypasses or speeds up the slow isomerization step. Circular dichroic spectral studies of the secondary structural changes of the native enzyme in the presence and absence of substrates supports the existence of conformational changes with NAD˙ and malate. Thus, a slow conformational change of the E:NAD:Mg complex is likely one of the rate-limiting steps in the pre-steady state.
Date: December 1991
Creator: Rajapaksa, Ranjani, 1949-
Partner: UNT Libraries

Linking Parent Relationships with Intimacy in a Selected Group of Young Adult University Students

Description: Literature suggests positive relationships with caregivers during childhood facilitate intimacy in young adulthood. The three hypotheses in this inquiry related to the students' relationship between parental intimacy and friend intimacy, gender differences in intimacy, and the perceived acceptance of parents. Subjects were 322 male and female university students, aged 17 through 25 years. Most were single, white, and middle class. During class the Children's Report of Parental Behavior, the Miller Social Intimacy Scale, and a demographic sheet were administered. ANOVA revealed that relationships between parental intimacy and friend intimacy were not significant. Females reported greater intimacy with friends than males. For both sexes, correlations between recalled parental intimacy and acceptance were higher for mothers than fathers.
Date: August 1991
Creator: Roland, Sandra Dodson
Partner: UNT Libraries

A Comprehensive Neuropsychological Screening Device for Adults: Reliability of Parallel Forms

Description: The purpose of the present study was to evaluate the reliability of parallel-forms of the Comprehensive Neuropsychological Screening Device (CNS). Forty-five subjects ranging in age from 16 to 69 were administered Form A and Form B of the CNS at two week intervals. Results indicated that the CNS has adequate test-retest reliability. The results suggest the applicability of using the CNS as a screening device for brain dysfunction.
Date: December 1991
Creator: Ganci, Maria
Partner: UNT Libraries

Concertino for Jazz Clarinet, Electric Viola and Symphonic Orchestra

Description: Concertino for Jazz Clarinet. Electric viola and Symphonic Orchestra is a composition of approximately fifteen minutes' duration, and is scored for two flutes (second doubling piccolo), two oboes, two Bb clarinets, two bassoons, four F- horns, two Bb trumpets, three trombones (third bass), two percussionists, solo Bb clarinet, solo electric viola and strings. The piece is divided into two movements; Andante and Canon. Concepts derived from jazz music are employed in, for example, harmony and improvisation in the solo parts, whereas the orchestration is mainly traditional. The piece is written for two great Swedish instrumentalists; Putte Wickman, clarinet, and Henrik Frendin, viola. Stylistically this work is difficult in the orchestral parts, since it uses concepts from two different musical styles, jazz and classical. Influences originate from such wide-ranging composers as Mozart, Stravinsky, Shostakovich and Chick Corea.
Date: August 1991
Creator: Holmquist, Mats G. (Mats Göran)
Partner: UNT Libraries

Luther the Augustinian: Augustine, Pelagianism and Luther's Philosophy of Man

Description: Augustine has had a large influence on the development of western theology, and nowhere is this more obvious that in Martin Luther's understanding of God, humankind and grace. Yet at the same time there are also significant differences in the two churchmen's thought. Sometimes these differences are subtle, such as their views of the state; other times they are not so subtle, such as their positions on free will or their praise of philosophy and its usefulness in sounding the depth of Christianity. In order to best explain these varying views, one must look at Augustine's and Luther's diverging opinions of man's nature where one will see that the dissimilarities are best understood in light of Luther's pessimistic view of humanity.
Date: August 1991
Creator: McGinnis, Jon D. (Jon David)
Partner: UNT Libraries

Chemical Mechanism of the Catalytic Subunit of Camp-Dependent Protein Kinase: Methods for Determining the Primary ¹⁸O Isotope Effects Using the Remote Label Technique

Description: A description of the nature of the transition state structure for phosphoryl transfer in the cAPK reaction requires a measurement of the primary 180 isotope effect at the serine hydroxyl acceptor. Since it is difficult to obtain primary 180 isotope effect directly, the 15N/1 4N ratio of the a-amine of the C-terminal glycine in the peptide Leu Arg-Lys-Ala-Ser-Leu-Gly (when serine is phosphorylated) was used to represent on the phosphorylation at serine. 15N Glycine, ' 4N-Glycine and 180 serine were synthesized and used to synthesize two peptides, one containing 1 80-serine/' 5 N glycine and second 1 60-serine/1 4N-glycine. Methods were developed for hydrolyzing the peptides and quantitatively isolating glycine. Partitioning results suggest that catalytic rate was slow compare to substrate dissociation. The 180 primary isotope effect will be determined in the near future using the method developed herein.
Date: December 1991
Creator: Chen, Gang, 1963-
Partner: UNT Libraries

Class and Freedom of Choice in the Marriage Patterns of Antebellum Texas Women

Description: Little scholarly analysis has been devoted to the hypothesis that antebellum Texas women generally married within their own socioeconomic (slaveholding) class, and thus had only limited choice in the selection of marriage partners. This quantitatively based investigation suggests that the popular image should be carefully qualified. This study reveals that although a majority of Texas women who married during the early 1850s chose men who had the same slaveholding status, a significant minority crossed class lines. By using marriage records of the period in correlation with information gleaned from the census, conclusions were reached. Contemporary women's diaries, letters and reminiscences were investigated, in addition to a historiography of marriage in the South, which created the background for this study.
Date: December 1991
Creator: Brown, Lisa (Lisa Christina)
Partner: UNT Libraries

A Real-Time Merging-Buffering Technique for MIDI Messages

Description: A powerful and efficient algorithm has been designed to deal with the critical timing problem of the MIDI messages. This algorithm can convert note events stored in a natural way to MIDI messages dynamically. Only limited memory space (the buffer) is required to finish the conversion work, and the size of the buffer is independent of the size of the original sequence (notes). This algorithm's real-time variable properties suggest not only the flexible real-time controls in the use of musical aspects, but also the expandability to interactive multi-media applications. A compositional environment called MusicSculptor has been implemented in terms of this algorithm.
Date: December 1991
Creator: Chang, Kuo-Lung
Partner: UNT Libraries

Pyrethroid Insecticide Effects on Bluegill Sunfish (Lepomis Macrochirus) and the Impacts of Bluegill Predation on Invertebrates in Microcosms

Description: Concurrent outdoor aquatic 1950 L microcosm and 0.04 ha mesocosm experiments with bluegill sunfish evaluated the ecological impact of cyfluthrin. Cyfluthrin effects were not observed on mesocosm bluegill; a slight decrease in growth was observed in the microcosm bluegill. Otolith weight to length relationships between bluegill size-classes from microcosms, local streams, and a fish hatchery revealed no differences. Our results indicated bluegill predation impacts were slight on benthic invertebrates. Extensive predation on emerging insects was observed. Microcosm bluegill impacts on zooplankton populations followed expected predation effects, resulting in larger populations of smaller taxa. Bluegill functioned as "keystone" predators for microcosm taxa and improved taxa richness for benthic colonizing invertebrates and zooplankton.
Date: May 1991
Creator: Morris, Rodney Gregg
Partner: UNT Libraries

Perception of Punitive Childhood Experiences, Adult Coping Mechanisms and Psychological Distress

Description: Differences in college student's psychological well-being, extrapunitiveness, and intropunitiveness were related to the presence or absence of maltreatment during childhood years, and its acknowledgement by the student. Subjects were 56 male and 85 female undergraduate students at the University of North Texas. Subjects were given structural scale v.3 of the California Psychological Inventory (CPI), the Extrapunitive (E), and Intropunitive (I) indices of the Hostility-Direction of Hostility Questionnaire (HDHQ), and the Physical Punishment scale (PP-scale) of the Assessing Environments Questionnaire (AEIII). Results indicate no significant differences in psychological well-being, extrapunitiveness, or intropunitiveness, which would be explained by the presence of maltreatment or its acknowledgement.
Date: December 1991
Creator: McCune, Linda Wheeler
Partner: UNT Libraries

The Precipitation of Strontium Sulfate in Gels

Description: The growth of strontium sulfate precipitate by diffusion in various gels was studied by using optical transmission and confocal microscopies, scanning and transmission electron microscopies, and energy dispersive X ray fluorescence. Pure silica gel, pure agarose gel and the silica/agarose mixed gel at pH 7 - 10 were used throughout the present study. Precipitate morphology is sensitive to pH and to the nature of the growth medium. The morphology was observed as a function of time. The lack of change is presumably because of rapid depletion of the limiting reagent after the very beginning of precipitation. The problem of separating strontium sulfate precipitate from the gel medium is discussed.
Date: December 1991
Creator: Lee, Ya
Partner: UNT Libraries

Personality Characteristics Considered Important for Children by Parents

Description: The primary research questions dealt with whether parents consider different personality characteristics of importance for boys and girls. Data were collected by conducting a telephone survey of a random sample of parents in the city of Denton with children under the age of eighteen living in the household. Respondents were asked whether they considered the personality characteristics of responsibility, strict obedience, being respectful of the opinions of others, showing good manners, being independent, and having loyalty to a religion not important, somewhat important, or very important for boys and girls. Of the respondents fifty-nine were fathers and one hundred and twenty-one were mothers. The analysis of the data revealed that mothers and fathers have similar attitudes concerning the importance of these personality characteristics.
Date: December 1991
Creator: Romm, Mary E. {4} (Mary Elizabeth)
Partner: UNT Libraries

The Cantatas of Jean-Philippe Rameau

Description: By the early eighteenth century, French music was tangibly influenced by the Italian style which had already permeated much of Europe. The French Cantata is symptomatic of that often disparaged influx. The cantatas of Rameau are a significant contribution to an important form. Written almost entirely in the early years of the artist's career, they hold details of his stylistic development. In the present study of Rameau's cantatas several aspects of his style are discussed as they relate both to his theoretic writings and to the various influences of the time. Examples of those stylistic elements found in the cantatas are cited and discussed. There is, as well, a comparison of the works to the poetic form standardized by Rousseau.
Date: May 1991
Creator: McManus, Catherine
Partner: UNT Libraries

A Comparison of Measures of Signal-To-Noise Ratio, Jitter, Shimmer, and Speaking Fundamental Frequency in Smoking and Nonsmoking Females

Description: Fifteen nonsmoking and fifteen smoking females 19 to 36 years of age were evaluated on measures of signal-to-noise ratio (SNR), jitter, shimmer, and speaking fundamental frequency (F0). The results indicated that: 1) there is a significant difference between female smokers and nonsmokers on measures of SNR, mean, and maximum F0 and, 2) there is no significant difference between female smokers and nonsmokers on measures of jitter, shimmer and minimum F0 . The SNR was found to be a powerful tool which is capable of distinguishing subtle vocal characteristics between the subject groups. It would appear that cigarette smoking may have an impact on the voice before distinct laryngeal pathologies are present.
Date: December 1991
Creator: Coy, Kelly (Kelly Bishop)
Partner: UNT Libraries

Interactions among Temperature, pH, and Cyfluthrin on Survival of the Fathead Minnow Pimephales promelas

Description: The 96-hr LC50 of cyfluthrin in Pimephales promelas ata temperature of 23*C and a pH of 8 was 1.08 g/L. The toxicity of cyfluthrin was inversely related to temperature and pH. A temperature of 10*C and a pH of 6 significantly decreased the 96-hr LC50 to 0.009 gg/L. Likewise, sublethal exposures to cyfluthrin significantly affected the fathead minnow's ability to tolerate high and low temperatures. Cyfluthrin compromised the fathead minnow's lower temperature tolerance (CTMin) by 60C and the upper temperature tolerance (CTMax) by 20C. Although cyfluthrin may not be present in the environment in large amounts due to its physical and chemical properties, small concentrations ( g/L) may adversely affect fish populations.
Date: December 1991
Creator: Heath, Susan M.
Partner: UNT Libraries

Mental Status, Intellectual, and Mood States Associated with Environmental Illness Patients

Description: The purpose of the present study was to begin development of a psychological profile for environmentally ill patients. Existing psychiatric labels are unable to encompass these patients. Test scores were drawn from a pool of 89 patients whose environmental exposures were verified by the presence of toxins in the blood serum. A Mental Status Exam, a Wechsler Adult Intelligence Scale-Revised screen, and the Profile of Mood States were administered. Results indicate a primary pattern which is significantly different from test norms consisting of fatigue, reduced mental functioning, and a lack of psychotic or personality disorder indicators. The reported symptoms of environmentally ill patients were objectively verified by current psychological test instruments. The need for a new diagnostic category for people who have been poisoned by environmental toxins is discussed.
Date: April 1991
Creator: Fincher, Cynthia Ellen
Partner: UNT Libraries