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Engineering Cardiac Organoid Vascularization via Fluid Shear Stress and Vascular-Promoting Growth Factors

Description: Cardiovascular disease (CVD) is the leading cause of death internationally. Efforts to decrease CVD death has been explored through stem cell technology, specifically organoid formation. Current cardiac organoid models lack the vascular networks for nutrient supply and maturation. In this study, pillar perfusion technology is used to fabricate cardiac organoids and induce vascularization via dynamic culturing and the addition of vascular promoting growth factors (GFs). In addition to this study… more
Date: August 2022
Creator: Huerta Gomez, Angello
Partner: UNT Libraries

Development of Biomimetic Human Lung Alveolus Chip

Description: The potential of physiologically relevant in vitro cell culture models for studying physiological and pathophysiological phenomena has been widely recognized as replacements for animal and conventional in vitro models. To create models that accurately replicate the structure and function of tissues and organs, it is essential to comprehend the biophysical and mechanical features of the extracellular matrix (ECM) and incorporate them into the in vitro cell culture models. Therefore, we first aim… more
Date: May 2023
Creator: Man, Kun
Partner: UNT Libraries

Differentiating Cardiac Organoids with Chamber Formations

Description: Considering that both cardiovascular disease (CVD) and congenital heart diseases (CHD) are still the leading cause of morbidity and mortality worldwide, there is a need for a robust and reliable cardiac model. Cardiac organoids are complex, three-dimensional cellular constructs that recapitulate the processes of the human embryonic heart. However, certain vital morphological features within the fetus are not yet replicable with cardiac organoids. Here we report our investigation to generate car… more
This item is restricted from view until August 1, 2024.
Date: July 2023
Creator: Seddoh, Percyval Prince-Danny
Partner: UNT Libraries
open access

Modeling of Hypertrophic Cardiomyopathy Using hiPSC-Derived Cardiomyocytes with Static Mechanical Stretching

Description: The heart is a dynamic environment that is constantly experiencing some degree of remodeling from the point of development, all the way through adulthood. While many genetic components may contribute to the overall presentation of hypertrophic cardiomyopathy (HCM), mutations occurring in sarcomere components such as myosin binding protein C3 (MYBPC3) are of the greatest popularity for study. Aiming to understand the mechanisms underlying heart diseases and to develop effective treatments that c… more
Date: May 2023
Creator: Rogozinski, Nicholas
Partner: UNT Libraries

Novel Phase-Chance Soft Actuators Controlled via Peltier

Description: Soft actuation methods are a developing field of robotics deemed suitable for physical human-robot interactions due to the adaptability of materials and compliant structures. Thermo-active soft actuators are a subset of these which convert thermal energy to mechanical work in the form of elongation, bending, or twisting to conform to the environment. This study is divided into three major studies that all use actuators with a working principle of phase-change fluid vaporizing for expansion with… more
Date: July 2023
Creator: Johnson, Daniel Cody
Partner: UNT Libraries
open access

Viability Study of Nylon-12 Carbon Fiber Filaments for Use in the Construction of a Powered Lower Body Exoskeleton via Fused Deposition Modeling by Means of Computer Simulation

Description: Members of the elderly population is disproportionately prone to experiencing mobility impairment due to their aging bodies and as a result have frail bodies that are at a higher risk of grave injury due to falling. In order to combat this assistive mobility devices such as exoskeletons have been developed to help patients enhance their range of motion. With additive manufacturing techniques, such as fused deposition modeling (FDM), becoming a more mainstream form of design, the inclusion of li… more
Date: May 2021
Creator: Joiner, Michael Andrew Lown
Partner: UNT Libraries
open access

In Vitro Electrochemical Evaluation of Bioelectronic Arrays

Description: In this paper, I sought to identify and develop a protocol on electrode arrays as a result of rapid aging by applying rapid current over time. We, however, apply a different approach by using phosphate buffer solution (PBS) to mimic the conditions of the body. Here we have established an in vitro protocol for accelerated aging, a process that involves testing in extreme conditions such as oxygen, heat, sunlight, humidity, and vibration aimed at speeding the normal aging process of items; on com… more
Date: December 2021
Creator: Singh, Sukhpreet
Partner: UNT Libraries
open access

Integrating a Brain Control Interface towards the Development of a Retrofitted, Low-Cost, Open Sourced, Electric Wheelchair

Description: The Emotiv Insight is a commercially available, low-cost, mobile EEG device that is commonly overshadowed by its costlier counterpart, the Emotiv EPOC. The purpose of this report is to investigate if the Emotiv Insight is a suitable headset to be used as a controlling factor in conjunction with an Arduino microcontroller and various electrical components that are used towards the development of an open-sourced, affordable electric wheelchair with the primary goal of providing those who either d… more
Date: August 2020
Creator: Ta, Christopher Ian
Partner: UNT Libraries
open access

Effects of Attachment Height and Rail Material of Resistance Training Sled on Trunk Lean and Jerk During Linear Acceleration Training

Description: Sprint acceleration training has been highly researched and found that resistance sleds are one of the most effective tools for maximizing training adaptations. The resistance sled is being used by many of the world leaders in athletic training but has yet to be researched for the kinetic and kinematic effects some of its key components cause. The aim of this study was to better understand the effects of the attachment height on the sled and sled rail material on the user's trunk lean and jerki… more
Date: May 2021
Creator: Fitzgerald, Sean
Partner: UNT Libraries
open access

Bio-Inspired Designs to Reduce Human-Exoskeleton Interaction to Prevent Falls in an Aging Population

Description: As a large generation ages, the collective financial and ethical responsibility to prevent egregious bodily harm through fall prevention and gait assistant exoskeleton devices increases. Risk for falls increases with age and the severity of the fall does as well. To support this elderly population, motorized exoskeletons can both increase stability as well as respond faster to fall scenarios, but current models do not more around the existing biological framework. Giving participants a range of… more
Date: August 2021
Creator: Gates, Edward Sean
Partner: UNT Libraries

Stem Cell Regulation Using Nanofibrous Membranes with Defined Structure and Pore Size

Description: Electrospun nanofibers have been researched extensively in the culturing of stem cells to understand their behavior since electrospun fibers mimic the native extracellular matrix (ECM) in many types of mammalian tissues. Here, electrospun nanofibers with defined structure (orientation/alignment) and pore size could significantly modulate human mesenchymal stem cell (hMSC) behavior. Controlling the fiber membrane pore size was predominantly influenced by the duration of electrospinning, while th… more
This item is restricted from view until September 1, 2024.
Date: August 2022
Creator: Blake, Laurence A
Partner: UNT Libraries

Nanolithographic Approaches to Probing Cell Membrane Modulation

Description: Metastatic cancer is more dangerous and difficult to treat than pre-metastatic cancer. Ninety percent of cancer-related deaths are caused by metastatic cancer. When cells go through metastases, they go through changes that allow them to break away from the primary tumor and invade secondary tissues. These changes, in lipid membrane composition and cellular glycocalyx, make the cell more resistant to therapeutics. Actin cytoskeleton contractility plays a major role in these changes, as increased… more
This item is restricted from view until June 1, 2024.
Date: May 2022
Creator: Mathis, Katelyn
Partner: UNT Libraries
open access

Modeling Hypertrophic Cardiomyopathy Using Genome-Edited Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Response to Dynamic Mechanotransduction

Description: Familial hypertrophic cardiomyopathy (HCM) is a genetic disease largely caused by a mutation in myosin binding protein C (MYBPC3) and it affects about 1:500 population leading to arrhythmic sudden death, heart failure, and atrial fibrillation. MYBPC3 activates calcium-induced actin-myosin filament sliding within the cardiac sarcomere, creating the force necessary for heart contraction. The underlying molecular mechanisms causing HCM phenotype remain elusive, therefore, there is an urgent need f… more
Date: May 2022
Creator: Strimaityte, Dovile
Partner: UNT Libraries
open access

Engineering Whole Cell-Based Biosensors for Heavy Metal Detection Using Metalloregulatory Transcriptional Repressors of the SmtB/ArsR Family

Description: This study focuses on engineering whole cell-based biosensors for heavy metal detection. Through the exploitation of metalloregulatory proteins, fabrication of metal ion-responsive biosensors is achieved. Metalloregulatory proteins of the SmtB/ArsR family including arsenite-responsive ArsR, cadmium-responsive CadC, zinc-responsive CzrA, and nickel-responsive NmtR were evaluated as biosensor sensing modules. Characterization of these four metal sensing modules was accomplished through quantifica… more
Date: May 2022
Creator: Draeger, Alison
Partner: UNT Libraries
open access

Parkinson's Disease and UPDRS-III Prediction Using Quiet Standing Data and Applied Machine Learning

Description: Parkinson's disease (PD) is a neurodegenerative disease that affects motor abilities with increasing severity as the disease progresses. Traditional methods for diagnosing PD require specialists scoring qualitative symptoms using the motor subscale of the Unified Parkinson's Disease Rating Scale (UPDRS-III). Using force-plate data during quiet standing (QS), this study uses machine learning to target the characterization and prediction of PD and UPDRS-III. The purpose of predicting different… more
Date: May 2021
Creator: Exley, Trevor Wayne
Partner: UNT Libraries

Engineering a Microfluidic Blood-Brain Barrier on a Silicon Chip

Description: The blood-brain barrier (BBB) is composed of brain microvascular endothelial cells (BMECs), pericytes, and astrocytic endfeet, which regulate the transport of molecules into and out of the brain. BMECs possess intrinsic barrier properties that limit the passage of approximately 98% of small molecules into the brain in healthy individuals. However, in some brain diseases, the BBB undergoes structural and functional alterations, which can contribute to disease progression. In this study, we aimed… more
Date: July 2023
Creator: Liu, Jiafeng
Partner: UNT Libraries

Development and Characterization of Compliant Bioelectronic Devices for Gastrointestinal Stimulation

Description: In this research, we aimed to develop thin-film devices on a polymer substrate and an alternative 3D-printed device with macroelectrodes for treating gastrointestinal (GI) conditions. First, the fabrication of thin-film devices was demonstrated on a softening thiol-ene/acrylate polymer utilizing titanium nitride (TiN) as electrode material. This was achieved by utilizing cleanroom fabrication processes such as photolithography, wet and dry etching. The functionality of the device was shown by p… more
This item is restricted from view until January 1, 2026.
Date: December 2023
Creator: Chitrakar, Chandani
Partner: UNT Libraries

Mesenchymal Stem Cells Encapsulated and Aligned in Self-Assembling Peptide Hydrogels

Description: This study presents a viable strategy using fmoc-protected peptides hydrogels, to encapsulate and stretch mesenchymal stem cells (MSC). To explore the peptide hydrogel potential, a custom mechanical stretching device with polydimethylsiloxane (PDMS) chambers were used to stretch MSCs encapsulated in Fmoc hydrogels. We investigated the impact of fmoc- FF prepared in dimethyl sulfoxide (DMSO), 1,1,1,3,3,3-hexafluoro-2-propanol (HFP) and deionizied water in the self-assembly, and mechanical proper… more
This item is restricted from view until January 1, 2025.
Date: December 2023
Creator: Kasani, Yashesh Varun
Partner: UNT Libraries

Magnetoresponsive Layer-by-Layer (LBL) Polyelectrolyte Microcapsules Exposed to Low Frequency Alternating Magnetic Field for Drug Delivery to Breast Cancer Cells

Description: Layer-by-layer (LBL) polyelectrolyte capsules can be modified to incorporate stimuli such as superparamagnetic nanoparticles which respond to a magnetic field only when it is turned on. Thus, they can act as a switch to load or unload their drug cargo on demand. Specifically, magnetite is incorporated into bilayer capsules made of alternating poly(allylamine hydrochloride) (PAH) and poly(sodium-p-styrenesulfonate) (PSS) which surrounds calcium carbonate core. The core is then dissolved using… more
This item is restricted from view until January 1, 2025.
Date: December 2023
Creator: Powell, Robert Darrel
Partner: UNT Libraries
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