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Regulation of Escherichia coli pyrBI Gene Expression in Pseudomonas fluorescens

Description: Pseudomonas fluorescens does not appear to regulate the enzymes of de novo pyrimidine biosynthesis at the level of gene expression. Little or no apparent repression of pyr gene expression is observed upon addition of exogenous pyrimidines to the growth medium. The Escherichia coli pyrBI genes for aspartate transcarbamoylase (ATCase) were sized down and cloned into the broad host range plasmid, pKT230. Upon introduction into a P.fluorescenspyrB mutant strain, ATCase showed repression in response… more
Date: May 1995
Creator: Shen, Weiping
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Progress towards DNA sequencing at the single molecule level

Description: We describe progress towards sequencing DNA at the single molecule level. Our technique involves incorporation of fluorescently tagged nucleotides into a targeted sequence, anchoring the labeled DNA strand in a flowing stream, sequential exonuclease digestion of the DNA strand, and efficient detection and identification of single tagged nucleotides. Experiments demonstrating strand specific exonuclease digestion of fluorescently labeled DNA anchored in flow as well as the detection of single cl… more
Date: December 1, 1995
Creator: Goodwin, P.M.; Affleck, R.L. & Ambrose, W.P.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Use of labeled primers for differential display

Description: The differential display of eukaryotic cDNAs using PCR allows for determination of mRNA species differentially expressed when comparing two similar cell populations. This procedure uses a (T){sub 12}XY oligonucleotide as the 3 ft primer and an arbitrary 8-10-mer as the 5 ft primer. Labeling occurs by inclusion of {alpha}[{sup 33}P]-dATP in the PCR reaction. Two artifacts caused by this approach are (1) random printing from dT present from affinity purification of PolyA+RNA and (2) hybridization… more
Date: February 1, 1995
Creator: Paunesku, T. & Woloschak, G. E.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

The gene identification problem: An overview for developers

Description: The gene identification problem is the problem of interpreting nucleotide sequences by computer, in order to provide tentative annotation on the location, structure, and functional class of protein-coding genes. This problem is of self-evident importance, and is far from being fully solved, particularly for higher eukaryotes, Thus it is not surprising that the number of algorithm and software developers working in this area is rapidly increasing. The present paper is an overview of the field, w… more
Date: March 27, 1995
Creator: Fickett, J.W.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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