120 Matching Results

Search Results

Advanced search parameters have been applied.
in subjects: "Enzymatic Hydrolysis"  
prev Results: 49 - 72 next
open access

The cost of ethanol production from lignocellulosic biomass -- A comparison of selected alternative processes. Final report

Description: The purpose of this report is to compare the cost of selected alternative processes for the conversion of lignocellulosic biomass to ethanol. In turn, this information will be used by the ARS/USDA to guide the management of research and development programs in biomass conversion. The report will identify where the cost leverages are for the selected alternatives and what performance parameters need to be achieved to improve the economics. The process alternatives considered here are not exhaust… more
Date: April 30, 1993
Creator: Grethlein, H.E. & Dill, T.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Citrus Waste Biomass Program

Description: Renewable Spirits is developing an innovative pilot plant bio-refinery to establish the commercial viability of ehtanol production utilizing a processing waste from citrus juice production. A novel process based on enzymatic hydrolysis of citrus processing waste and fermentation of resulting sugars to ethanol by yeasts was successfully developed in collaboration with a CRADA partner, USDA/ARS Citrus and Subtropical Products Laboratory. The process was also successfully scaled up from laboratory… more
Date: January 30, 2007
Creator: Grohman, Karel & Stevenson, Scott
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Development of Geothermally Assisted Process for Production of Liquid Fuels and Chemicals from Wheat Straw

Description: Recently there has been much interest in developing processes for producing liquid fuels from renewable resources. The most logical long term approach in terms of economics derives the carbohydrate substrate for fermentation from the hydrolysis of cellulosic crop and forest residues rather than from grains or other high grade food materials (1,2). Since the presence of lignin is the main barrier to the hydrolysis of cellulose from lignocellulosic materials, delignification processes developed b… more
Date: June 1, 1981
Creator: Murphy, V. G.; Linden, J. C.; Moreira, A. R. & Lenz, T. G.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

[Characterization of lignin and Mn peroxidases from Phanerochaete chrysosporium]. Progress report

Description: Lignin peroxidases were investigated with respect to enzyme kinetics and NMR spectroscopy of the heme domain. MN peroxidases were studied with respect to the role of oxalate in enzyme activity, the NMR spectroscopy of the heme domain. Gene expression of both lignin and MN peroxidases were examined as well as expression of site-directed mutants aimed at scale up production of these enzymes.
Date: December 31, 1992
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis for Corn Stover

Description: This report is an update of NREL's ongoing process design and economic analyses of processes related to developing ethanol from lignocellulosic feedstocks. The U.S. Department of Energy (DOE) is promoting the development of ethanol from lignocellulosic feedstocks as an alternative to conventional petroleum-based transportation fuels. DOE funds both fundamental and applied research in this area and needs a method for predicting cost benefits of many research proposals. To that end, the National … more
Date: June 1, 2002
Creator: Aden, A.; Ruth, M.; Ibsen, K.; Jechura, J.; Neeves, K.; Sheehan, J. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis Current and Futuristic Scenarios

Description: The National Renewable Energy Laboratory (NREL) has undertaken a complete review and update of the process design and economic model for the biomass-to-ethanol enzymatic based process. The process design includes the core technologies being researched by the U.S. Department of Energy (DOE): prehydrolysis, simultaneous saccharification and co-fermentation, and cellulase enzyme production. In addition, all ancillary areas--feed handling, product recovery and purification, wastewater treatment lig… more
Date: July 20, 1999
Creator: Wooley, Robert; Ruth, Mark; Sheehan, John; Ibsen, Kelly; Majdeski, Henry & Galvez, Adrian
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover

Description: This report describes one potential biochemical ethanol conversion process, conceptually based upon core conversion and process integration research at NREL. The overarching process design converts corn stover to ethanol by dilute-acid pretreatment, enzymatic saccharification, and co-fermentation. Building on design reports published in 2002 and 1999, NREL, together with the subcontractor Harris Group Inc., performed a complete review of the process design and economic model for the biomass-to-… more
Date: March 1, 2011
Creator: Humbird, D.; Davis, R.; Tao, L.; Kinchin, C.; Hsu, D.; Aden, A. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Overcoming the Recalcitrance of Cellulosic Biomass by Value Prior to Pulping: Cooperative Research and Development Final Report, CRADA Number CRD-07-221

Description: The Value Prior to Pulping (VPP) project goal was to demonstrate the technical and commercial feasibility of introducing a new value stream into existing pulp and paper mills. Essentially the intent was to transfer the energy content of extracted hemicellulose from electricity and steam generated in the recovery boiler to a liquid transportation fuel. The hemicellulose fraction was extracted prior to pulping, fractionated, or conditioned if necessary, and fermented to ethanol. Commercial adapta… more
Date: April 1, 2012
Creator: Lowell, A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Improved Biorefinery for the Production of Ethanol, Chemicals, Animal Feed and Biomaterials From Sugar Cane

Description: The Audubon Sugar Institute (ASI) of Louisiana State University’s Agricultural Center (LSU AgCenter) and MBI International (MBI) sought to develop technologies that will lead to the development of a sugar-cane biorefinery, capable of supplying fuel ethanol from bagasse. Technology development focused on the conversion of bagasse, cane-leaf matter (CLM) and molasses into high value-added products that included ethanol, specialty chemicals, biomaterials and animal feed; i.e. a sugar cane-based bi… more
Date: January 29, 2009
Creator: Day, Donal F.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

New bern biomass to energy project Phase I: Feasibility study

Description: Weyerhaeuser, together with Amoco and Carolina Power & Light, performed a detailed evaluation of biomass gasification and enzymatic processing of biomass to ethanol. This evaluation assesses the potential of these technologies for commercial application to determine which technology offers the best opportunity at this time to increase economic productivity of forest resources in an environmentally sustainable manner. The work performed included preparation of site-specific plant designs that in… more
Date: October 1, 1995
Creator: Parson, F. & Bain, R.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Enzymatic studies of radiation damage. Progress report, July 1, 1976-September 30, 1977

Description: The major accomplishments of the year were a new method and a totally unexpected discovery that venom phosphodiesterase belongs to the group of single-strand specific nucleases. It acts on viral duplex DNA by producing a series of shorter fragments of duplex DNA. It was also found that the relaxed circular DNA of PM2 virus, that was previously enzymatically ligated, may be opened by very high amounts of phosphodiesterase. The new method deals with a simplified way of preparing polynucleotide ki… more
Date: June 1, 1977
Creator: Laskowski, M., Sr.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Transgenic Plants Lower the Costs of Cellulosic Biofuels (Fact Sheet)

Description: A new transgenic maize was observed to be less recalcitrant than wild-type biomass, as manifested through lower severity requirements to achieve comparable levels of conversion. Expression of a single gene derived from bacteria in plants has resulted in transgenic plants that are easier and cheaper to convert into biofuels. Part of the high production cost of cellulosic biofuels is the relatively poor accessibility of substrates to enzymes due to the strong associations between plant cell wall … more
Date: November 1, 2011
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Partnering with Industry to Advance Biofuels and Bioproducts (Fact Sheet)

Description: Fact sheet describing NREL's Integrated Biorefinery Research Facility, a biochemical pilot plant and partnership facility containing equipment and lab space for pretreatement, enzymatic hydrolysis, fermentation, compositional analysis, and downstream processing. For more than 30 years, the U.S. Department of Energy's (DOE) National Renewable Energy Laboratory (NREL) has been at the leading edge of research and technology advancements to develop renewable fuels and bioproducts. NREL works to dev… more
Date: December 1, 2011
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Kinetic and Modeling Investigation to Provide Design Guidelines for the NREL Dilute-Acid Process Aimed at Total Hydrolysis/Fractionation of Lignocellulosic Biomass: July 1998

Description: Following up on previous work, subcontractor investigated three aspects of using NREL ''pretreatment'' technology for total hydrolysis (cellulose as well as hemicellulose) of biomass. Whereas historic hydrolysis of biomass used either dilute acid or concentrated acid technology for hydrolysis of both hemicellulose and cellulose, NREL has been pursuing very dilute acid hydrolysis of hemicellulose followed by enzymatic hydrolysis of cellulose. NREL's countercurrent shrinking-bed reactor design fo… more
Date: August 1, 2004
Creator: Lee, Y. Y.; Iyer, P.; Xiang, Q. & Hayes, J.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Mechanisms of interaction of radiation with matter. Progress report, July 1, 1991--August 31, 1992

Description: This project is concerned with studies of biological activity-structure relationships in which the mechanisms of interaction of ionizing radiation and benzopyrene (PB) compounds with DNA are being investigated and compared. Emphasis is focused on effects of DNA conformation on its mechanisms of interaction with ionizing radiation, on the influence of structure and stereochemistry of BP metabolites on mechanisms of DNA damage, and on influence of DNA conformation on interactions between BP metab… more
Date: August 31, 1992
Creator: Geacintov, N. E. & Pope, M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Screening Study for Waste Biomass to Ethanol Production Facility Using the Amoco Process in New York State. Final Report

Description: This report evaluates the economic feasibility of locating biomass-to-ethanol waste conversion facilities in New York State. Part 1 of the study evaluates 74 potential sites in New York City and identifies two preferred sites on Staten, the Proctor Gamble and the Arthur Kill sites, for further consideration. Part 2 evaluates upstate New York and determines that four regions surrounding the urban centers of Albany, Buffalo, Rochester, and Syracuse provide suitable areas from which to select spec… more
Date: August 1, 1995
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

[Induction of polygalacturonases important in pathogenicity of Pseudomonas solanacearum]

Description: Recent studies on the importance of hydroxyproline-rich glycoproteins (HPRG's) in the nature and function of plant cell walls have led to the question as to whether proteolytic enzymes are also involved in tissue maceration and act in concert with other cell wall degrading enzymes in the process. The primary objective of this research was to determine whether proteolytic enzymes, in combination with other enzymes, are involved in the degradation of plant cell walls and thus may be essential for… more
Date: January 1, 1992
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Carboxyl group reactivity in actin

Description: While earlier work showed that the carboxyl groups of proteins could be quantitatively coupled to amino groups at pH 4.75 in the presence of EDC and a denaturing agent, the work presented here indicates that under milder conditions the modification of sidechain carboxyls is limited and somewhat specific. Most of the incorporated glycine ethyl ester (GEE) is apparently bound to five carboxyls. The total GEE incorporated was 3 to 4 moles/mole of protein as measured by an increase in Gly upon acid… more
Date: January 1, 1986
Creator: Elzinga, M.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

NREL Proves Cellulosic Ethanol Can Be Cost Competitive (Fact Sheet)

Description: Ethanol from non-food sources - known as "cellulosic ethanol" - is a near-perfect transportation fuel: it is clean, domestic, abundant, and renewable, and it can potentially replace 30% of the petroleum consumed in the United States, but its relatively high cost has limited its market. That changed in 2012, when the National Renewable Energy Laboratory (NREL) demonstrated the technical advances needed to produce cellulosic ethanol at a minimum ethanol selling price of $2.15/gallon (in 2007 doll… more
Date: November 1, 2013
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Evaluation of the Natick enzymatic hydrolysis process for use in the production of ethanol from municipal solid waste or from wood. Final report

Description: Economic evaluation of a conceptual, large-scale, commercial ethanol production facility using the enzymatic hydrolysis technology are presented. Designs and cost estimates for the mechanical processing and the ethanol fermentation and recovery are included. Production of ethanol from both wood and solid wastes is covered. (MHR)
Date: October 1, 1979
Creator: Jones, J. L.; Fong, W. S. & Chatterjee, A. K.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Fundamental study of the mechanism and kinetics of cellulose hydrolysis by acids and enzymes. Progress report, June 1, 1975--December 31, 1975

Description: This project deals with acidic and enzymatic hydrolysis of cellulosic materials. Highlights of the first eight months of out project are as follows. (1) Essentially homogeneous C/sub 1/, C/sub x/, and Cellobiase enzyme have been isolated from Cellulase-Onozuka of Trichodeima viride origin. Besides the 3 major components, one protein of a molecular weight of 10,000 was purified, which has strong C/sub x/ activity and also another (M.W. 23,000) with strong C/sub 1/ activity. (2) Kinetics of Cello… more
Date: February 1, 1976
Creator: Tsao, G T
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Final Technical Report DOE/GO/13142-1

Description: This research adds to the understanding of the areas of residual starch and biomass conversion to alcohol, by providing data from pilot plant equipment of larger scale than the minimum required to give commercially scalable data. Instrumentation and control is in place to capture the information produced, for economic and technical evaluation. The impact of rheology, recycle streams, and residence time distributions on the technical and economic performance can be assessed. Various processes ca… more
Date: September 15, 2010
Creator: Mulvihill, Patrick & Nguyen, Quang
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Fundamental study of the mechanism and kinetics of cellulose hydrolysis by acids and enzymes. Final report, June 1, 1978-January 31, 1981

Description: There are three basic enzymes (e.g., endoglucanase (C/sub x/), exoglucanase (C/sub 1/) and cellobiase) comprising the majority of extracellular cellulase enzymes produced by the cellulolytic mycelial fungi, Trichoderma reesei, and other cellulolytic microorganisms. The enzymes exhibited different mode of actions in respect to the hydrolysis of cellulose and cellulose derived oligosaccharides. In combination, these enzymes complimented each other to hydrolyze cellulose to its basic constituent, … more
Date: February 1, 1981
Creator: Gong, C. S. & Chang, M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Mechanisms of interaction of radiation with matter. Progress report, July 1, 1992--June 30, 1993

Description: This project is concerned with the mechanisms by which polynuclear aromatic (PNA) compounds on the one hand, and ionizing radiation on the other, cause damage to DNA. PNA compounds constitute an important class of environmental pollutants derived from energy-related sources which, upon metabolic activation to diolepoxide derivatives, produce bulky PNA-DNA lesions interfere with the normal DNA replication and transcription processes, and give rise to mutations and the initiation of tumors. Chira… more
Date: June 30, 1993
Creator: Geacintov, N. E. & Pope, M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
Back to Top of Screen