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Functional characterization of dna repair proteins

Description: Genetic material (DNA) is susceptible to spontaneous decomposition, to attack by reactive chemicals produced naturally in cells, and to assault by environmental and food mutagens. DNA modifications can lead to permanent genetic changes that promote human disease. To combat the deleterious effects of DNA damage, organisms are equipped with DNA repair systems. The focus of our investigations has been to elucidate the details of mammalian DNA repair. These studies have provided important insights … more
Date: February 16, 2000
Creator: Wilson, D. M., III
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

DNA Testing in Criminal Justice: Background, Current Law, Grants, and Issues

Description: This report provides an overview of how DNA is used to investigate crimes and help protect the innocent. It also reviews current statutory law on collecting DNA samples, sharing DNA profiles generated from those samples, and providing access to post-conviction DNA testing. The report also includes a summary of grant programs authorized by Congress to assist state and local governments with reducing DNA backlogs, provide post-conviction DNA testing, and promote new technology in the field. It al… more
Date: May 2, 2011
Creator: James, Nathan
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Genomic Sequencing of Single Microbial Cells from Environmental Samples

Description: Recently developed techniques allow genomic DNA sequencing from single microbial cells [Lasken RS: Single-cell genomic sequencing using multiple displacement amplification, Curr Opin Microbiol 2007, 10:510-516]. Here, we focus on research strategies for putting these methods into practice in the laboratory setting. An immediate consequence of single-cell sequencing is that it provides an alternative to culturing organisms as a prerequisite for genomic sequencing. The microgram amounts of DNA re… more
Date: February 1, 2008
Creator: Ishoey, Thomas; Woyke, Tanja; Stepanauskas, Ramunas; Novotny, Mark & Lasken, Roger S.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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The effect of solvent dynamics on the low frequency collectivemotions of DNA in solution and unoriented films

Description: Infrared spectroscopy is used to probe the dynamics of invitro samples of DNA prepared as solutions and as solid unoriented films.The lowest frequency DNA mode identified in the far-infrared spectra ofthe DNA samples is found to shift in frequency when the solvent influencein the hydration shell is altered. The lowest frequency mode also hascharacteristics that are similar to beta - relaxations identified inother glass forming polymers.
Date: April 20, 2006
Creator: Woods, K.N.; Lee, S.A.; Holman, H.-Y.N. & Wiedemann, H.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

DNA Testing in Criminal Justice: Background, Current Law, Grants, and Issues

Description: This report provides an overview of how DNA is used to investigate crimes and help protect the innocent. It also reviews current statutory law on collecting DNA samples, sharing DNA profiles generated from those samples, and providing access to post-conviction DNA testing. The report also includes a summary of grant programs authorized by Congress to assist state and local governments with reducing DNA backlogs, provide post-conviction DNA testing, and promote new technology in the field. It al… more
Date: January 9, 2014
Creator: James, Nathan
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

DNA Testing in Criminal Justice: Background, Current Law, Grants, and Issues

Description: This report provides an overview of how DNA is used to investigate crimes and help protect the innocent. It also reviews current statutory law on collecting DNA samples, sharing DNA profiles generated from those samples, and providing access to post-conviction DNA testing. The report also includes a summary of grant programs authorized by Congress to assist state and local governments with reducing DNA backlogs, provide post-conviction DNA testing, and promote new technology in the field.
Date: December 6, 2012
Creator: James, Nathan
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Viruses of eukaryotic green algae. Final technical report, June 1, 1989--February 1, 1992

Description: We have isolated and partially characterized many large, polyhedral, DNA containing, plaque forming viruses which infect certain unicellular, eukaryotic, chlorella-like green algae. These viruses have several unique features, including the fact that they code for DNA site-specific endonucleases and DNA methyltransferases. The primary objectives of this study were to identify, clone, and characterize some of the virus-encoded DNA methyltransferases and DNA restriction endonucleases in order to u… more
Date: December 31, 1992
Creator: Van Etten, J. L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Method for priming and DNA sequencing

Description: A method is presented for improving the priming specificity of an oligonucleotide primer that is non-unique in a nucleic acid template which includes selecting a continuous stretch of several nucleotides in the template DNA where one of the four bases does not occur in the stretch. This also includes bringing the template DNA in contract with a non-unique primer partially or fully complimentary to the sequence immediately upstream of the selected sequence stretch. This results in polymerase-med… more
Date: December 1, 1997
Creator: Mugasimangalam, R. C. & Ulanovsky, L. E.
Item Type: Refine your search to only Patent
Partner: UNT Libraries Government Documents Department
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Coupling sequencing by hybridization (SBH) with gel sequencing for an inexpensive analysis of genes and genomes

Description: The speed and cost of DNA sequencing are bottlenecks in the analysis of genes end genomes. Sequencing by hybridization (SBH) is a versatile method with several applications which can accelerated DNA screening, mapping and sequencing. Requirements, achievements and problems in the development of the SBH format 1 (DNA samples arrayed) are presented and schemes for its synergetic coupling with gel sequencing techniques are discussed. It appears that by one hybridization machine with 24 boxes and f… more
Date: November 1, 1996
Creator: Drmanac, S.; Labat, I.; Hauser, B. & Drmanac, R.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Disruption of Maternal DNA Repair Increases Sperm-DerivedChromosomal Aberrations

Description: The final weeks of male germ cell differentiation occur in aDNA repair-deficient environment and normal development depends on theability of the egg to repair DNA damage in the fertilizing sperm. Geneticdisruption of maternal DNA double-strand break repair pathways in micesignificantly increased the frequency of zygotes with chromosomalstructural aberrations after paternal exposure to ionizing radiation.These findings demonstrate that radiation-induced DNA sperm lesions arerepaired after fertil… more
Date: February 7, 2007
Creator: Marchetti, Francesco; Essers, Jeroun; Kanaar, Roland & Wyrobek,Andrew J.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Abstracts of papers presented at the LVIII Cold Spring Harbor Symposium on quantitative Biology: DNA and chromosomes

Description: This volume contains the abstracts of oral and poster presentations made at the LVIII Cold Spring Harbor Symposium on Quantitative Biology entitles DNA & Chromosomes. The meeting was held June 2--June 9, 1993 at Cold Spring Harbor, New York.
Date: December 31, 1993
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Contribution to Sequencing of the Deinococcus radiodurans Genome

Description: The stated goal of this project was to supply The Institute for Genomic Research (TIGR) with pure DNA from the bacterium Deinocmus radiodurans RI for purposes of complete genomic sequencing by TIGR. We subsequently decided to expand this project to include a second goal; this second goal was the development of a NotI chromosomal map of D. radiodurans R1 using Pulsed Field Gel Electrophoresis (PFGE).
Date: March 11, 1999
Creator: Minton, K. W.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

An integrated multiple capillary array electrophoresis system for high-throughput DNA sequencing

Description: A capillary array electrophoresis system was chosen to perform DNA sequencing because of several advantages such as rapid heat dissipation, multiplexing capabilities, gel matrix filling simplicity, and the mature nature of the associated manufacturing technologies. There are two major concerns for the multiple capillary systems. One concern is inter-capillary cross-talk, and the other concern is excitation and detection efficiency. Cross-talk is eliminated through proper optical coupling, good … more
Date: March 27, 1998
Creator: Lu, X.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Three dimensional imaging of DNA fragments during electrophoresis using a confocal detector

Description: We have measured the three dimensional distribution of DNA fragments within an electrophoretic band. The measurements were made using a confocal microscope and a photon counting photomultiplier detector. A DNA sequencing standard was loaded into glass microchannel plates containing polyacrylamide gel. The measurements were made by scanning the plates in three dimensions using a mechanical stage under computer control, while electrophoresis was taking place. We found that the distribution of DNA… more
Date: January 30, 1995
Creator: Brewer, Laurence R.; Davidson, Courtney; Balch, Joe & Carrano, Anthony
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Structural Studies of MJ1529, an O6-methylguanine-DNAMethyltransferase

Description: The structure of an O{sub 6}-methylguanine methyltransferase from the thermophile Methanococcus jannaschii has been determined using multinuclear multidimensional NMR spectroscopy. The structure is similar to homologues from other organisms that have been determined by crystallography, with some variation in the N-terminal domain. The C-terminal domain is more highly conserved in both sequence and structure. Regions of the protein show broadening reflecting conformational flexibility that is li… more
Date: January 10, 2006
Creator: Roberts, Anne; Pelton, Jeffrey G. & Wemmer, David E.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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[Studies of the repair of radiation-induced genetic damage in Drosophila]. Annual progress report, June 1, 1992--June 30, 1993

Description: The most interesting discovery made over the past year derives from sequence analysis of cDNAs from the putative mus308 gene. The theoretical translation product of this gene contains a DNA polymerase domain near the carboxy terminus and DNA/RNA helicase motifs near the amino terminus. There is currently no precedent in the literature for a single polypeptide containing both of these domains. The protein appears to be a novel DNA repair enzyme which should be fruitful ground for future enzymolo… more
Date: September 1, 1998
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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FY05 LDRD Fianl Report Investigation of AAA+ protein machines that participate in DNA replication, recombination, and in response to DNA damage LDRD Project Tracking Code: 04-LW-049

Description: The AAA+ proteins are remarkable macromolecules that are able to self-assemble into nanoscale machines. These protein machines play critical roles in many cellular processes, including the processes that manage a cell's genetic material, but the mechanism at the molecular level has remained elusive. We applied computational molecular modeling, combined with advanced sequence analysis and available biochemical and genetic data, to structurally characterize eukaryotic AAA+ proteins and the protei… more
Date: December 4, 2006
Creator: Sawicka, D; de Carvalho-Kavanagh, M S; Barsky, D & Venclovas, C
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

PROTEOLYTIC REMOVAL OF THE CARBOXYL TERMINUS OF THE T4 GENE 32 HELIX-DESTABILIZING PROTEIN ALTERS THE T4 IN VITRO REPLICATION COMPLEX

Description: The proteolytic removal of about 60 amino acids from the COOH terminus of the bacteriophage T4 helix-destabilizing protein (gene 32 protein) produces 32*I, a 27,000-dalton fragment which still binds tightly and cooperatively to single-stranded DNA. The substitution of 32*I protein for intact 32 protein in the seven-protein T4 replication complex results in dramatic changes in some of the reactions catalyzed by this in vitro DNA replication system, while leaving others largely unperturbed. (1) L… more
Date: July 1, 1980
Creator: Burke, R.L.; Alberts, B.M. & Hosoda, J.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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DOE project on genome mapping and sequencing. Progress report, 1992

Description: These efforts on the human genome project were initiated in September, 1990, to contribute towards completion of the human genome project physical mapping effort. In the original application, the authors proposed a novel strategy for constructing a physical map of human chromosome 11, based upon techniques derived in this group and by others. The original goals were to (1) produce a set of cosmid reference clones mapped to specific sites by high resolution fluorescence in situ hybridization, (2… more
Date: December 31, 1992
Creator: Evans, G.A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Neutron scattering and nuclear magnetic resonance spectroscopy structural studies of protein-DNA complexes

Description: This is the final report of a one-year, Laboratory-Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). The project sought to employ advanced biophysical measurements to study the structure of nucleosomes and the structure of origins of DNA replication. The fundamental repeating unit of human chromosomes is the nucleosome, which contains about 200 base pairs of DNA and 9 histone proteins. Genome replication is strictly associated with the reversible ace… more
Date: March 1, 1996
Creator: Bradbury, E.M.; Catasti, P.; Chen, X.; Gupta, G.; Imai, B.; Moyzis, R. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Development of High Throughput Process for Constructing 454 Titanium and Illumina Libraries

Description: We have developed two processes with the Biomek FX robot to construct 454 titanium and Illumina libraries in order to meet the increasing library demands. All modifications in the library construction steps were made to enable the adaptation of the entire processes to work with the 96-well plate format. The key modifications include the shearing of DNA with Covaris E210 and the enzymatic reaction cleaning and fragment size selection with SPRI beads and magnetic plate holders. The constructio… more
Date: May 28, 2010
Creator: Deshpande, Shweta; Hack, Christopher; Tang, Eric; Malfatti, Stephanie; Ewing, Aren; Lucas, Susan et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Large Gap Size Paired-end Library Construction for Second Generation Sequencing

Description: Fosmid or BAC end sequencing plays an important role in de novo assembly of large genomes like fungi and plants. However construction and Sanger sequencing of fosmid or BAC libraries are laborious and costly. The current 454 Paired-End (PE) Library and Illumina Jumping Library construction protocols are limited with the gap sizes of approximately 20 kb and 8 kb, respectively. In the attempt to understand the limitations of constructing PE libraries with greater than 30Kb gaps, we have purified … more
Date: May 28, 2010
Creator: Peng, Ze; Hamilton, Matthew; Froula, Jeff; Ewing, Aren; Foster, Brian & Cheng, Jan-Fang
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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