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The Genome of the Western Clawed Frog Xenopus tropicalis

Description: The western clawed frog Xenopus tropicalis is an important model for vertebrate development that combines experimental advantages of the African clawed frog Xenopus laevis with more tractable genetics. Here we present a draft genome sequence assembly of X. tropicalis. This genome encodes over 20,000 protein-coding genes, including orthologs of at least 1,700 human disease genes. Over a million expressed sequence tags validated the annotation. More than one-third of the genome consists of transp… more
Date: October 1, 2009
Creator: Hellsten, Uffe; Harland, Richard M.; Gilchrist, Michael J.; Hendrix, David; Jurka, Jerzy; Kapitonov, Vladimir et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Invariability of Central Metabolic Flux Distribution in Shewanella oneidensis MR-1 Under Environmental or Genetic Perturbations

Description: An environmentally important bacterium with versatile respiration, Shewanella oneidensis MR-1, displayed significantly different growth rates under three culture conditions: minimal medium (doubling time {approx} 3 hrs), salt stressed minimal medium (doubling time {approx} 6 hrs), and minimal medium with amino acid supplementation (doubling time {approx}1.5 hrs). {sup 13}C-based metabolic flux analysis indicated that fluxes of central metabolic reactions remained relatively constant under the t… more
Date: April 21, 2009
Creator: Tang, Yinjie; Martin, Hector Garcia; Deutschbauer, Adam; Feng, Xueyang; Huang, Rick; Llora, Xavier et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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A probe-based mapping strategy for DNA sequencing with mobile primers. Progress report

Description: Research on DNA sequencing continued. The specific areas of research targeted for the period of this Progress Report included three general phases: (1) optimization of probe-mapping by both the development of new transposons and the design of stream-lined methods for mapping; (2) application of transposon-based methods to larger plasmids and cosmids; and (3) initiation of PCR-based applications of transposons.
Date: December 31, 1991
Creator: Strausbaugh, L. D. & Berg, C. M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Drosophila by the dozen

Description: This year's conference on Drosophila research illustratedwell the current focus of Drosophila genomics on the comprehensiveidentification of functional elements in the genome sequence, includingmRNA transcripts arising from multiple alternative start sites and splicesites, a multiplicity of noncoding transcripts and small RNAs,identification of binding sites for transcription factors, sequenceconservation in related species and sequence variation within species.Resources and technologies for ge… more
Date: July 13, 2007
Creator: Celniker, Susan E. & Hoskins, Roger A.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Transposon Tagging of Disease Resistance Genes. Progress Report, May 1, 1988--1992

Description: Our goal is to clone genes in lettuce determining resistance to downy mildew. One approach involves the mobilization of transposons into resistance genes to mutate and tag the target gene. Because transposons have yet to be isolated and characterized from lettuce, the majority of our experiments have involved Ac from corn as this is increasingly the best characterized transposon. Over the past several years, various labs have contributed to a detailed understanding of the biology of Ac in corn … more
Date: June 1, 1994
Creator: Michelmore, R.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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First international E. coli genome meeting

Description: This volume is a collection of abstracts of oral presentations and poster sessions of studies reported at the First International E. Coli Genome Meeting, held September 10-14, 1992 at the University of Wisconsin.
Date: December 31, 1992
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Transposon Tagging of Disease Resistance Genes. Final Report, May 1, 1988--April 30, 1993

Description: The goal of this project was to develop a transposon mutagenesis system for lettuce and to clone and characterize disease resistance genes by transposon tagging. The majority of studies were conducted with the Ac/Ds System. Researchers made and tested several constructs as well as utilized constructions shown to be functional in other plant species. Researchers demonstrated movement of Ac and DS in lettuce; however, they transposed at much lower frequencies in lettuce than in other plant specie… more
Date: September 1, 1994
Creator: Michelmore, R.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Growth and development of maize that contains mutant tubulin genes

Description: Mutant maize plants containing a Mu transposon disrupting one of the five beta tubulin genes of interest were followed for several generations and hybridized with each other to produce plants containing disruptions in both copies of a single gene or disruption of more than one tubulin gene. Seedlings of some of these plants were grown under chilling conditions for a few weeks. After DOE funding ended, plants have been assessed to see whether mutant are more or less tolerant to chilling. Other m… more
Date: July 23, 2004
Creator: Wick, Susan M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

A probe-based mapping strategy for DNA sequencing with mobile primers

Description: Research on DNA sequencing continued. The specific areas of research targeted for the period of this Progress Report included three general phases: (1) optimization of probe-mapping by both the development of new transposons and the design of stream-lined methods for mapping; (2) application of transposon-based methods to larger plasmids and cosmids; and (3) initiation of PCR-based applications of transposons.
Date: January 1, 1991
Creator: Strausbaugh, L. D. & Berg, C. M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

[Studies of the repair of radiation-induced genetic damage in Drosophila]. Annual progress report, September 1, 1990--July 1, 1991

Description: Research is focused on the following areas: characterization of DNA double-strand break repair; using injected oligonucleotides as templates to repair double-strand DNA breaks; analysis of a gene required for postreplication repair; cloning of a gene required for resistance to DNA cross-linking agents; cloning of a gene required for excision repair; cloning of a gene required for X-ray resistance; and transposon tagging DNA repair genes.
Date: December 31, 1991
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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[Studies of the repair of radiation-induced genetic damage in Drosophila]. Annual progress report, June 1, 1989--September 1, 1990

Description: The most exciting discovery made over the past year derives from an analysis of the interaction between DNA repair and P-element transposition. A powerful new system was developed for analyzing the repair of DNA double-strand breaks. A screen was completed of mutagenized autosomes obtained from two San Francisco laboratories with the recovery of several mutants that will provide the foundation for future efforts to clone repair related genes. At the same time, strong progress has been made in t… more
Date: December 31, 1990
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Bio-Engineering High Performance Microbial Strains for MEOR

Description: The main objectives of this three-year research project are: (1) to employ the latest advances in genetics and bioengineering, especially Directed Protein Evolution technology, to improve the effectiveness of the microbial enhanced oil recovery (MEOR) process. (2) to improve the surfactant activity and the thermal stability of bio-surfactant systems for MEOR; and (3) to develop improved laboratory methods and tools that screen quickly candidate bio-systems for EOR. Biosurfactants have been rece… more
Date: December 30, 2007
Creator: Fang, Xiangdong; Wang, Qinghong & Shuler, Patrick
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Global Regulatory Pathways in the Alphaproteobacteria

Description: A major goal for microbiologists in the twenty-first century is to develop an understanding of the microbial cell in all its complexity. In addition to understanding the function of individual gene products we need to focus on how the cell regulates gene expression at a global level to respond to different environmental parameters. Development of genomic technologies such as complete genome sequencing, proteomics, and global comparisons of mRNA expression patterns allows us to begin to address … more
Date: April 27, 2007
Item Type: Refine your search to only Text
Partner: UNT Libraries Government Documents Department
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Detection of Cell Wall Chemical Variation in Zea Mays Mutants Using Near-Infrared Spectroscopy

Description: Corn stover is regarded as the prime candidate feedstock material for commercial biomass conversion in the United States. Variations in chemical composition of Zea mays cell walls can affect biomass conversion process yields and economics. Mutant lines were constructed by activating a Mu transposon system. The cell wall chemical composition of 48 mutant families was characterized using near-infrared (NIR) spectroscopy. NIR data were analyzed using a multivariate statistical analysis technique c… more
Date: January 1, 2001
Creator: Buyck, N. & Thomas, S.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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The BDGP gene disruption project: Single transposon insertions associated with 40 percent of Drosophila genes

Description: The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each Drosophila gene by the insertion of a single transposable element. As part of this effort, transposons in more than 30,000 fly strains were localized and analyzed relative to predicted Drosophila gene structures. Approximately 6,300 lines that maximize genomic coverage were selected to be sent to the Bloomington Stock Center for public distribution, bringing the size of the BDGP gene disruption collection to 7,140 lines. It n… more
Date: January 13, 2004
Creator: Bellen, Hugo J.; Levis, Robert W.; Liao, Guochun; He, Yuchun; Carlson, Joseph W.; Tsang, Garson et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Final report

Description: The originally funded project was geared to pursue research on regulation of photosystem II (PSII) in the cyanobacterium Synechococcus elongatus PCC 7942. We characterized a locus, psfR, (psbA stimulating factor) that affects expression of the psbAI gene, which encodes the PSII protein D1. Over-expression of psfR, which encodes a protein with receiver and pseudo-receiver domains, acts at the promoter region to elevate expression of psbAI and a subset of other loci. We reoriented the remainder o… more
Date: March 31, 2005
Creator: Golden, Susan S.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

The fifth international conference on Arabidopsis research

Description: This volume contains abstracts of oral and poster presentations made in conjunction with the Fifth International Conference on Arabidopsis Research held August 19--22, 1993 at the Ohio State University, Columbus, Ohio.
Date: December 31, 1993
Creator: Hangarter, R.; Scholl, R.; Davis, K. & Feldmann, K.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Transposon-induced nuclear mutations that alter chloroplast gene expression. Annual report, September 1, 1992--April 15, 1993

Description: The goal of this project is to use mutant phenotypes as a guide to nuclear genes that control the timing and cell-type specificity of chloroplast gene expression. Studies are being conducted with nuclear mutants of maize that are defective in the biogenesis or translation of chloroplast mRNAs. Currently studies are focused on two nuclear mutants with specific and unique lesions in chloroplast RNA processing (crp mutants). Crp1 mutants (formerly called hcf136) fail to accumulate the cytochrome f… more
Date: April 20, 1993
Creator: Barkan, A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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A genetic analysis of Adhl regulation. Progress report, June 1991--May 1993

Description: Several separate but related studies are reported on the mechanism of alcohol dehydrogenase (Adh-1) are reported. A study of a deletion mutation in the TATA box region which resulted in an increase from 6--60% of wildtype Adh-1 expression in the revertant has led to a focus on trans-acting protein factors that bind the TATA box. Analysis of another revertant has led to study of cis-acting sequences in Adh-1 expression. Screening efforts aimed at defining different mutants affecting Adh-1 expres… more
Date: December 1, 1992
Creator: Freeling, M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Advanced sequenching technology

Description: This progress report briefly describes the laboratory's advances in chemiluminescent detectors for sequencing DNA, sequence reading algorithm, and for automation of sequencing.(DT)
Date: January 1, 1992
Creator: Gesteland, R. F.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

First international E. coli genome meeting

Description: This volume is a collection of abstracts of oral presentations and poster sessions of studies reported at the First International E. Coli Genome Meeting, held September 10-14, 1992 at the University of Wisconsin.
Date: January 1, 1992
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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[Genetics and biochemistry of surfactant synthesis in Rhodococcus sp. H13-A]. Final report

Description: The rationale for these studies resides is that biosurfactant synthesis occurs only when cells are grown with alkanes as sole source of carbon and energy. It is reasoned that biosurfactant synthesis is linked genetically to alkane oxidation and that the identification and characterization of the alk genes would provide information about the structural and regulator genes involved, in biosurfactant synthesis. Rhodococcus H13-A chromosomal DNA was isolated and digested with the restriction endonu… more
Date: December 31, 1989
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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The mechanism of switching from an acidogenic to butanol-acetone fermentation by Clostridium acetobutylicum. Technical progress report, July 1990--December 1992

Description: The overall objective of this project is to elucidate the detailed mechanism by which solvent-forming bacteria such as Clostridium acetobutylicum regulate the well-known shift in fermentation pathway between alcohol-acetone and organic acid production. It is desired to eventually isolate and describe: (1) the regulatory genes and protein elements that determine induction of synthesis of the solvent-pathway enzymes; and (2) how this regulation system interacts with the sporulatin induction and d… more
Date: December 31, 1992
Creator: Rogers, P.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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