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Boronated monoclonal antibody conjugates for neutron capture therapy

Description: This paper describes the effectiveness of /sup 10/B-labeled monoclonal antibodies against Colo-38 human melanoma in vitro. The authors obtained high boron to antibody ratios while maintaining antibody activity by using dextran intermediate carriers to link /sup 10/B to the antibody. They developed a double cell quasi-competitive binding bioassay to minimize the effects of nonspecific binding of boronated complexes to cells. 1 fig., 2 tabs.
Date: January 1, 1986
Creator: Borg, D.C.; Elmore, J.J. Jr. & Ferrone, S.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Survey of immunoassay techniques for biological analysis

Description: Immunoassay is a very specific, sensitive, and widely applicable analytical technique. Recent advances in genetic engineering have led to the development of monoclonal antibodies which further improves the specificity of immunoassays. Originally, radioisotopes were used to label the antigens and antibodies used in immunoassays. However, in the last decade, numerous types of immunoassays have been developed which utilize enzymes and fluorescent dyes as labels. Given the technical, safety, health… more
Date: October 1, 1986
Creator: Burtis, C.A.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Methods for environmental monitoring of DOE waste disposal and storage sites. Semiannual progress report, November 1, 1985--March 31, 1986

Description: This progress report contains an account of recent research efforts carried out at the Oak Ridge Research Institute, to raise antibodies in New Zealand White Rabbits which are specific for various strains of Thiobacillus ferrooxidans bacteria. It is then intended to use the antibodies, in a subsequent phase of the project, to develop one or more ELISAs, which would be used to analyze, both in the laboratory and in the field, the numbers and extent of dispersion of these microorganisms at acid m… more
Date: December 31, 1986
Creator: Blake, R. & Revis, N.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Detection of polyaromatic compounds using antibody-based fiberoptics fluoroimmunosensors

Description: In this work we have investigated the performance of an antibody-based fiberoptics sensor for the detection of the carcinogen benzo(a)pyrene and its DNA-adduct product BP-tetrol. The excellent sensitivity of this device - femtomole limits of detection for BP - illustrates that it has considerable potential to perform analyses of chemical and biological samples at trace levels in complex matrices. The results indicate that fiberoptics-based fluoroimmunosensors can be useful in a wide spectrum of… more
Date: January 1, 1987
Creator: Vo-Dinh, T.; Tromberg, B. J.; Griffin, G. D.; Ambrose, K. R.; Sepaniak, M. J. & Alarie, J. P.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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DOE contractor's meeting on chemical toxicity

Description: The Office of Health and Environmental Research (OHER) is required to determine the potential health and environmental effects associated with energy production and use. To ensure appropriate communication among investigators and scientific disciplines that these research studies represent, OHER has sponsored workshops. This document provides a compilation of activities at the Third Annual DOE/OHER Workshop. This year's workshop was broadened to include all OHER activities identified as within … more
Date: January 1, 1987
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Lactation-induced cadmium-binding proteins

Description: Previously we have demonstrated an increase during midlactation in /sup 109/Cd adsorption and increased retention by the duodenum, kidney, and mammary tissue of mouse dams receiving environmental levels of cadmium//sup 109/Cd via drinking water, with little change in /sup 109/Cd retention in liver and jejunum compared to nonpregnant controls. Results are reported here of a study of cadmium deposition during midlactation as associated with induction of metallothionein (MT). A cadmium/hemoglobin … more
Date: January 1, 1987
Creator: Bhattacharyya, M.H.; Solaiman, D.; Garvey, J.S. & Miyazaki, W.Y.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Feasibility studies of using the Catfish Immune System to produce monoclonal antibodies

Description: The objective of these studies was to determine the feasibility of using a teleost cell line to produce monoclonal antibodies. Studies were undertaken to demonstrate the production of a polyclonal response of channel catfish (Icatalurus punctatus) challenged with mycotoxins coupled to a protein carrier. Companion studies were also performed to induce a permanent cell line with catfish lymphocytes. Attempts to demonstrate a polyclonal response to haptenized mycotoxins were inconclusive. Tests to… more
Date: March 1, 1987
Creator: Poston, T. M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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ELISA-Based Segregation of Adult Spring Chinook Salmon for Control of Bacterial Kidney Disease, Annual Report FY 1989.

Description: Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum, is a serious disease of salmonid fish worldwide. The disease has a major impact on spring chinook salmon populations in the Columbia River system. There is strong evidence that R. safmoninarum can be transmitted from parent to progeny, and therefore culling of gametes from infected parents should obviate this mode of transmission. This report presents the results from the first year of our four year study to investigate segre… more
Date: December 1, 1989
Creator: Kaattari, Stephen L. & Winton, James R.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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An Immunochemical Approach to the Study of DNA Damage and Repair, Technical Progress Report: 1989-1990

Description: The overall objective of this project is to produce antibodies to unique modified DNA bases and develop immunochemical assays to quantitate these lesions in damaged DNA. During this past year we have developed an antibody and chemical test to quantitate a basic sites in DNA and produced antibodies to the 8-oxopurines. This report discusses the detection of a basic sites in DNA and the preparation of antibodies to 8-hydroxyadenine and 8-hydroxyguanine.
Date: November 14, 1990
Creator: Wallace, Susan S. & Erlanger, Bernard F.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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ELISA-Based Segregation of Adult Spring Chinook Salmon for Control of Bacterial Kidney Disease, Annual Report FY 1990.

Description: Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum (RS), is a serious disease of salmonid fish worldwide. The disease has a major impact on spring chinook salmon populations in the Columbia River system. There is strong evidence that RS can be transmitted from parent to progeny, and segregation of progeny based on levels of antigen detected in adult fish may obviate this mode of transmission. Results from the second year of a four year study to investigate segregation of brood… more
Date: December 1, 1990
Creator: Winton, James R. & Kaattari, Stephen L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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(Controls of the plant endomembrane-secretory pathway)

Description: These studies are focused on elucidating the molecular structure of plant cell membranes with special reference to cell surface glycoproteins. The studies reported herein include use of monoclonal antibodies to characterize cell surface epitopes, construction of cDNA libraries of cell surface proteins, isolation of plant cell mutants by flow cytometry, detection of beta-glucouronidase marker enzyme systems in plants, expression go VSVG (the major envelope glycoprotein of Vesicular Stomatis Viru… more
Date: January 1, 1991
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Phytochromes in Photosynthetically Competent Plants

Description: Major improvements have been made in the purification of green-oat phytochrome. An effective protease inhibitor has been incorporated, the scale of preparations has been increased greatly, an immunodominant contaminant has been eliminated, and the extent of purification has been increased by at least a factor of ten. Five new MAbs and rabbit PAbs to green-oat phytochrome, as well as rabbit PAbs to a synthetic, putative green-oat phytochrome peptide, have been produced and characterized, togethe… more
Date: January 1, 1991
Creator: Pratt, L.H.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Molecular biology of Lea genes of higher plants

Description: This report contains our progress to date in determining the function of the D-7 Lea proteins in cotton embryos. We have completely sequenced the D-7 gene and established {ital E. coli} transformants which synthesize reasonable amounts of the D-7 protein. Two-dimensional electrophoresis was required to assay fractions for D-7 protein during purification to homogeneity, since D-7 has no known enzymatic activity, contains no Trp, and little Phe or Tyr, and {ital E. coli} has several proteins of s… more
Date: July 1, 1991
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Waste minimization for commercial radioactive materials users generating low-level radioactive waste

Description: The objective of this document is to provide a resource for all states and compact regions interested in promoting the minimization of low-level radioactive waste (LLW). This project was initiated by the Commonwealth of Massachusetts, and Massachusetts waste streams have been used as examples; however, the methods of analysis presented here are applicable to similar waste streams generated elsewhere. This document is a guide for states/compact regions to use in developing a system to evaluate a… more
Date: July 1, 1991
Creator: Fischer, D. K.; Gitt, M.; Williams, G. A.; Branch, S. (EG and G Idaho, Inc., Idaho Falls, ID (United States)); Otis, M. D.; McKenzie-Carter, M. A. et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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An immunochemical approach to the study of DNA damage and repair

Description: The overall objective of this project is to produce antibodies to unique modified DNA bases and develop immunochemical assays to quantitate these lesions in damaged DNA. During this past year we have characterized antibodies to 8-oxopurines, produced novel antibodies to 5-hydroxyuracil and developed new methodologies to increase our level of sensitivity of detection. 7 refs., 5 figs.
Date: August 1, 1991
Creator: Wallace, S.S.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

[Controls of the plant endomembrane-secretory pathway]. Final report

Description: These studies are focused on elucidating the molecular structure of plant cell membranes with special reference to cell surface glycoproteins. The studies reported herein include use of monoclonal antibodies to characterize cell surface epitopes, construction of cDNA libraries of cell surface proteins, isolation of plant cell mutants by flow cytometry, detection of beta-glucouronidase marker enzyme systems in plants, expression go VSVG (the major envelope glycoprotein of Vesicular Stomatis Viru… more
Date: December 31, 1991
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Phytochromes in Photosynthetically Competent Plants. Final Report

Description: Major improvements have been made in the purification of green-oat phytochrome. An effective protease inhibitor has been incorporated, the scale of preparations has been increased greatly, an immunodominant contaminant has been eliminated, and the extent of purification has been increased by at least a factor of ten. Five new MAbs and rabbit PAbs to green-oat phytochrome, as well as rabbit PAbs to a synthetic, putative green-oat phytochrome peptide, have been produced and characterized, togethe… more
Date: December 31, 1991
Creator: Pratt, L. H.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Capillary liquid chromatography using laser-based and mass spectrometric detection. [Capillary zone electrophoresis (CZE); micellar electrokinetic capillary kchromatography (MECC)]

Description: In the years following the 1986 seminal paper (J. Chromatogr. Sci., 24, 347-352) describing modern capillary zone electrophoresis (CZE), the prominence of capillary electrokinetic separation techniques has grown. A related electrochromatographic technique is micellar electrokinetic capillary chromatography (MECC). This report presents a brief synopsis of research efforts during the current 3-year period. In addition to a description of analytical separations-based research, results of efforts t… more
Date: January 1, 1992
Creator: Sepaniak, M.J. & Cook, K.D.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Immunological techniques as tools to characterize the subsurface microbial community at a trichloroethylene contaminated site

Description: Effective in situ bioremediation strategies require an understanding of the effects pollutants and remediation techniques have on subsurface microbial communities. Therefore, detailed characterization of a site's microbial communities is important. Subsurface sediment borings and water samples were collected from a trichloroethylene (TCE) contaminated site, before and after horizontal well in situ air stripping and bioventing, as well as during methane injection for stimulation of methane-utili… more
Date: January 1, 1992
Creator: Fliermans, C.B.; Dougherty, J.M.; Franck, M.M.; McKinzey, P.C. & Hazen, T.C.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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(The interaction of ferredoxin:NADP sup + oxidoreductase and ferredoxin:thioredoxin reductase with substrates)

Description: We seek to map the ferredoxin-binding sites on three soluble enzymes located in spinach chloroplasts which utilize ferredoxin as an electron donor:Ferredoxin:NADP{sup +}oxidoreductase (FNR); ferredoxin:thioredoxin reductase (FTR) and glutamate synthase. As the availability of amino acid sequences for the enzymes are important in such studies, that the amino acid sequence of glutamate synthase needs be determined, the amino acid sequences of FNR, FTR and ferredoxin are already known. Related to … more
Date: January 1, 1992
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

An immunochemical approach to the study of DNA damage and repair

Description: The overall objective of this project has been to develop immunochemical methods to quantitate unique DNA base damages in order to facilitate studies on radiation-induced damage production and repair. Specifically, we have been using antibodies raised to damaged bases to quantitate unique lesions in model systems in order to evaluate their potential biological consequences. Our approach has been to synthesize modified nucleotides or nucleosides, conjugate them to protein carriers, and use the c… more
Date: May 1, 1992
Creator: Wallace, S.S. (Vermont Univ., Burlington, VT (United States). Dept. of Microbiology and Molecular Genetics) & Erlanger, B.F. (Columbia Univ., New York, NY (United States). Dept. of Microbiology)
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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An immunochemical approach to the study of DNA damage and repair. Technical progress report, May 1, 1989--April 30, 1992

Description: The overall objective of this project has been to develop immunochemical methods to quantitate unique DNA base damages in order to facilitate studies on radiation-induced damage production and repair. Specifically, we have been using antibodies raised to damaged bases to quantitate unique lesions in model systems in order to evaluate their potential biological consequences. Our approach has been to synthesize modified nucleotides or nucleosides, conjugate them to protein carriers, and use the c… more
Date: May 1, 1992
Creator: Wallace, S. S. & Erlanger, B. F.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Augmented Fish Health Monitoring in Idaho, 1990-1991 Annual Report.

Description: The Idaho augmented fish health monitoring contract DE-A179-87BP65903 was awarded in June 1987 and fully implemented in January 1988. The fourth annual report of activities serviced under this contract is presented. The prevailing fish health problems in 1990 include persistent infections caused by Mvxobolus cerebralis and Flexibacter osvchronhilus. Subclinical infections of Renibacterium salmoninarum have been confirmed in pools of chinook kidney tissues using Enzyme-linked immunosorbant assay… more
Date: August 1, 1992
Creator: Munson, A. Douglas
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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