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in subjects: "In-Situ Hybridization"

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Supersensitive in Situ Hybridization by Tyramide Signal Amplification and Nanogold Silver Staining: The Contribution of Autometallography and Catalyzed Reporter Deposition to the Rejuvenation of In Situ Hybridization

It is peculiar that in situ hybridization (ISH), a technique with many similarities to immunohistochemistry (IHC), has not enjoyed the phenomenal growth in both basic research and clinical applications as has its sister technique IHC. Since the late 1970s, when immunoperoxidase techniques began to be applied to routine diagnostic material and to numerous research applications, there has been a natural evolution of the IHC procedure. Namely, only a few primary antibodies were available commercia… more

Date: April 17, 2002

Creator: Tubbs, Raymond R.; Pettay, James; Grogan, Thomas; Powell, R. D.; Cheung, Annie L. M.; Hainfeld, James et al.

Item Type: Book

Partner: UNT Libraries Government Documents Department

open access

Single Molecule Techniques for Advanced in situ Hybridization

One of the most significant achievements of modern science is completion of the human genome sequence, completed in the year 2000. Despite this monumental accomplishment, researchers have only begun to understand the relationships between this three-billion-nucleotide genetic code and the regulation and control of gene and protein expression within each of the millions of different types of highly specialized cells. Several methodologies have been developed for the analysis of gene and protein … more

Date: February 3, 2003

Creator: Hollars, C. W.; Stubbs, L.; Carlson, K.; Lu, X. & Wehri, E.

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Combined Fluorescent and Gold Probes for Microscopic and Morphological Investigations.

Nanogold{reg_sign}, a gold cluster with a core of gold atoms 1.4 nm in diameter, has proven to be a superior probe label for electron microscopy (EM), giving both higher labeling density and improved access to previously hindered or restricted antigens. It may be visualized by autometallography (AMG) for use in light microscopy (LM): silver-and gold-amplified Nanogold detection has proven to be one of the most sensitive methods available for the detection of low copy number targets such as vira… more

Date: April 17, 2002

Creator: Powell, R. D. & Hainfeld, J. F.

Item Type: Book

Partner: UNT Libraries Government Documents Department

open access

Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

Fluorescence in situ hybridization (FISH) is a sensitive and rapid procedure to detect gene rearrangements in tumor cells using non-isotopically labeled DNA probes. Large insert recombinant DNA clones such as bacterial artificial chromosome (BAC) or P1/PAC clones have established themselves in recent years as preferred starting material for probe preparations due to their low rates of chimerism and ease of use. However, when developing probes for the quantitative analysis of rearrangements invo… more

Date: December 4, 2008

Creator: Greulich-Bode, Karin M.; Wang, Mei; Rhein, Andreas P.; Weier, Jingly F. & Weier, Heinz-Ulli G.

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

BAC-FISH assays delineate complex chromosomal rearrangements in a case of post-Chernobyl childhood thyroid cancer

Structural chromosome aberrations are known hallmarks of many solid tumors. In the papillary form of thyroid cancer (PTC), for example, activation of the receptor tyrosine kinase (RTK) genes, RET and neurotrophic tyrosine kinase receptor type I (NTRK1) by intra- and interchromosomal rearrangements has been suggested as a cause of the disease. However, many phenotypically similar tumors do not carry an activated RET or NTRK-1 gene or express abnormal ret or NTRK-1 transcripts. Thus, we hypothesi… more

Date: March 9, 2009

Creator: Kwan, Johnson; Baumgartner, Adolf; Lu, Chun-Mei; Wang, Mei; Weier, Jingly F.; Zitzelsberger, Horst F. et al.

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Molecular stress response in the CNS of mice after systemic exposureto interferon-alpha, ionizing radiation and ketamine

We previously showed that the expression of troponin T1 (Tnnt 1) was induced in the central nervous system (CNS) of adultmice 30 min after treatment with ketamine, a glutamate N-methyl-D-aspartic acid (NMDA) receptor antagonist. We hypothesized that Tnnt 1 expression may be an early molecular biomarker of stress response in the CNS of mice. To further evaluate this hypothesis, we investigated the regional expression of Tnnt 1 in the mouse brain using RNA in situ hybridization 4 h after systemic… more

Date: March 3, 2009

Creator: Lowe, Xiu R.; Marchetti, Francesco; Lu, Xiaochen & Wyrobek, Andrew J.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

A sequence-based survey of the complex structural organization of tumor genomes

The genomes of many epithelial tumors exhibit extensive chromosomal rearrangements. All classes of genome rearrangements can be identified using End Sequencing Profiling (ESP), which relies on paired-end sequencing of cloned tumor genomes. In this study, brain, breast, ovary and prostate tumors along with three breast cancer cell lines were surveyed with ESP yielding the largest available collection of sequence-ready tumor genome breakpoints and providing evidence that some rearrangements may b… more

Date: April 3, 2008

Creator: Collins, Colin; Raphael, Benjamin J.; Volik, Stanislav; Yu, Peng; Wu, Chunxiao; Huang, Guiqing et al.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Chromosomal mosaicism in mouse two-cell embryos after paternal exposure to acrylamide

Chromosomal mosaicism in human preimplantation embryos is a common cause ofspontaneous abortions, however, our knowledge of its etiology is limited. We used multicolor fluorescence in situ hybridization (FISH) painting to investigate whether paternally-transmitted chromosomal aberrations result in mosaicism in mouse 2-cell embryos. Paternal exposure to acrylamide, an important industrial chemical also found in tobacco smoke and generated during the cooking process of starchy foods, produced sig… more

Date: October 14, 2008

Creator: Marchetti, Francesco; Bishop, Jack; Lowe, Xiu & Wyrobek, Andrew J

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Validation of DNA probes for molecular cytogenetics by mapping onto immobilized circular DNA

Date: December 16, 2008

Creator: Greulich-Bode, Karin; Wang, Mei; Rhein, Andreas; Weier, Jingly & Weier, Heinz-Ulli

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Chromosome-specific DNA Repeat Probes

In research as well as in clinical applications, fluorescence in situ hybridization (FISH) has gained increasing popularity as a highly sensitive technique to study cytogenetic changes. Today, hundreds of commercially available DNA probes serve the basic needs of the biomedical research community. Widespread applications, however, are often limited by the lack of appropriately labeled, specific nucleic acid probes. We describe two approaches for an expeditious preparation of chromosome-specific… more

Date: March 16, 2006

Creator: Baumgartner, Adolf; Weier, Jingly Fung & Weier, Heinz-Ulrich G.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Evolutionary expansion and divergence in a large family of primate-specific zinc finger transcription factor genes

Although most genes are conserved as one-to-one orthologs in different mammalian orders, certain gene families have evolved to comprise different numbers and types of protein-coding genes through independent series of gene duplications, divergence and gene loss in each evolutionary lineage. One such family encodes KRAB-zinc finger (KRAB-ZNF) genes, which are likely to function as transcriptional repressors. One KRAB-ZNF subfamily, the ZNF91 clade, has expanded specifically in primates to compri… more

Date: September 28, 2005

Creator: Hamilton, A T; Huntley, S; Tran-Gyamfi, M; Baggott, D; Gordon, L & Stubbs, L

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Molecular cytogenetic characterization of a human thyroid cancercell line

The incidence of papillary thyroid carcinoma (PTC) increases significantly after exposure of the head and neck region to ionizing radiation, yet we know neither the steps involved in malignant transformation of thyroid epithelium nor the specific carcinogenic mode of action of radiation. Such increased tumor frequency became most evident in children after the 1986 nuclear accident in Chernobyl, Ukraine. In the twelve years following the accident, the average incidence of childhood PTCs (chPTC) … more

Date: January 4, 2006

Creator: Weier, Heinz-Ulrich G.; Tuton, Tiffany B.; Ito, Yuko; Chu, LisaW.; Lu, Chung-Mei; Baumgartner, Adolf et al.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Molecular cytogenetic analysis of human blastocysts andcytotrophoblasts by multi-color FISH and Spectra Imaging analyses

Numerical chromosome aberrations in gametes typically lead to failed fertilization, spontaneous abortion or a chromosomally abnormal fetus. By means of preimplantation genetic diagnosis (PGD), we now can screen human embryos in vitro for aneuploidy before transferring the embryos to the uterus. PGD allows us to select unaffected embryos for transfer and increases the implantation rate in in vitro fertilization programs. Molecular cytogenetic analyses using multi-color fluorescence in situ hybri… more

Date: February 8, 2006

Creator: Weier, Jingly F.; Ferlatte, Christy; Baumgartner, Adolf; Jung,Christine J.; Nguyen, Ha-Nam; Chu, Lisa W. et al.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

TECHNICAL REPORT

Because this DOE grant was abruptly terminated without warning, this group was not able to accomplish the insertion of the biosensor genes into the mouse lines. They have been able to generate some of the mouse lines but have not been able to complete the ones that would give them the model systems that would allow them to investigate metastasis real-time in living tumors at the cellular level. Nonetheless, until the loss of funding, they have made progress in applications of the equipment to b… more

Date: October 11, 2007

Creator: SINGER, DR. ROBERT

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Induction of Genomic Instability In Vivo by Low Doses of 137Cs gamma rays

The overall goal of this project is to determine if low doses (below or equal to the level traditionally requiring human radiation protection, i.e. less than or equal to 10 cGy) of low LET radiation can induce genomic instability. The magnitude of genomic instability was measured as delayed chromosome instability in bone marrow cells of exposed mice with different levels of endogenous DNA-dependent protein kinase catalytic subunit (DNA-PKcs) activity, i.e. high (C57BL/6J mice), intermediate (BA… more

Date: January 6, 2006

Creator: Rithidech, Kanokporn; Simon, Sanford, R. & Whorton, Elbert, B.

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Rapid evolution of a recently retroposed transcription factor YY2 in mammalian genomes

YY2 was originally identified due to its unusual similarity to the evolutionarily well conserved, zinc-finger gene YY1. In this study, we have determined the evolutionary origin and conservation of YY2 using comparative genomic approaches. Our results indicate that YY2 is a retroposed copy of YY1 that has been inserted into another gene locus named Mbtps2 (membrane-bound transcription factor protease site 2). This retroposition is estimated to have occurred after the divergence of placental mam… more

Date: November 11, 2005

Creator: Luo, C.; Lu, X.; Stubbs, L. & Kim, J.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Chromosomal Rainbows detect Oncogenic Rearrangements of Signaling Molecules in Thyroid Tumors

Altered signal transduction can be considered a hallmark of many solid tumors. In thyroid cancers the receptor tyrosine kinase (rtk) genes NTRK1 (Online Mendelian Inheritance in Man = OMIM *191315, also known as 'TRKA'), RET ('Rearranged during Transfection protooncogene', OMIM *164761) and MET (OMIM *164860) have been reported as activated, rearranged or overexpressed. In many cases, a combination of cytogenetic and molecular techniques allows elucidation of cellular changes that initiate tumo… more

Date: August 19, 2010

Creator: O'Brien, Benjamin; Jossart, Gregg H.; Ito, Yuko; Greulich-Bode, Karin M.; Weier, Jingly F.; Munne, Santiago et al.

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Somatic genomic variations in extra-embryonic tissues

In the mature chorion, one of the membranes that exist during pregnancy between the developing fetus and mother, human placental cells form highly specialized tissues composed of mesenchyme and floating or anchoring villi. Using fluorescence in situ hybridization, we found that human invasive cytotrophoblasts isolated from anchoring villi or the uterine wall had gained individual chromosomes; however, chromosome losses were detected infrequently. With chromosomes gained in what appeared to be a… more

Date: May 21, 2010

Creator: Weier, Jingly F.; Ferlatte, Christy & Weier, Heinz-Ulli G.

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

Preaxial Polydactyly in Sost/Sostdc1 Double Knockouts

In the United States, {approx}5% are born with congenital birth defects due to abnormal function of cellular processes and interactions. Sclerosteosis, a rare autosomal recessive disease, causes hyperostosis of the axial and appendicular skeleton, and patients present radial deviation, digit syndactyly, nail dysplasia, and overall high bone mineral density. Sclerosteosis is due to a loss of function of sclerostin (Sost). Sost is a Wnt (abbrev.) antagonist; when mutated, nonfunctional Sost resul… more

Date: July 29, 2011

Creator: Yee, C M; Collette, N M & Loots, G G

Item Type: Report

Partner: UNT Libraries Government Documents Department

open access

A BAC-based physical map of the Drosophila buzzatii genome

Large-insert genomic libraries facilitate cloning of large genomic regions, allow the construction of clone-based physical maps and provide useful resources for sequencing entire genomes. Drosophilabuzzatii is a representative species of the repleta group in the Drosophila subgenus, which is being widely used as a model in studies of genome evolution, ecological adaptation and speciation. We constructed a Bacterial Artificial Chromosome (BAC) genomic library of D. buzzatii using the shuttle vec… more

Date: March 18, 2005

Creator: Gonzalez, Josefa; Nefedov, Michael; Bosdet, Ian; Casals, Ferran; Calvete, Oriol; Delprat, Alejandra et al.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

In situ quantification of genomic instability in breast cancer progression

Genomic instability is a hallmark of breast and other solid cancers. Presumably caused by critical telomere reduction, GI is responsible for providing the genetic diversity required in the multi-step progression of the disease. We have used multicolor fluorescence in situ hybridization and 3D image analysis to quantify genomic instability cell-by-cell in thick, intact tissue sections of normal breast epithelium, preneoplastic lesions (usual ductal hyperplasia), ductal carcinona is situ or invas… more

Date: May 15, 2003

Creator: Ortiz de Solorzano, Carlos; Chin, Koei; Gray, Joe W. & Lockett, Stephen J.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

A system for combined three-dimensional morphological and molecular analysis of thick tissue specimens

We present a new system for simultaneous morphological and molecular analysis of thick tissue samples. The system is composed of a computer assisted microscope and a JAVA-based image display, analysis and visualization program that allows acquisition, annotation, meaningful storage, three-dimensional reconstruction and analysis of structures of interest in thick sectioned tissue specimens. We describe the system in detail and illustrate its use by imaging, reconstructing and analyzing two compl… more

Date: April 25, 2002

Creator: Fernandez-Gonzalez, Rodrigo; Jones, Arthur; Garcia-Rodriguez, Enrique; Yuan Chen, Ping; Idica, Adam; Lockett, Stephen J. et al.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Identification of a third protein 4.1 tumor suppressor, protein 4.1R, in meningioma pathogenesis

Meningiomas are common tumors of the central nervous system, however, the mechanisms under lying their pathogenesis are largely undefined. Two members of the Protein 4.1 super family, the neuro fibromatosis 2 (NF2) gene product (merlin/schwannomin) and Protein 4.1B have been implicated as meningioma tumor suppressors. In this report, we demonstrate that another Protein 4.1 family member, Protein 4.1R, also functions as a meningioma tumor suppressor. Based on the assignment of the Protein 4.1R g… more

Date: June 11, 2003

Creator: Robb, Victoria A.; Li, Wen; Gascard, Philippe; Perry, Arie; Mohandas, Narla & Gutmann, David H.

Item Type: Article

Partner: UNT Libraries Government Documents Department

open access

Dissection of Molecular Mechanisms Regulating Protein Body Formation in Maize Endosperm - DE-FG03-95-ER20183 B139

Dissection of Molecular Mechanisms Regulating Protein Body Formation in Maize Endosperm - DE-FG03-95-ER20183 Final Technical Report and Patent Summary Dr. Brian A. Larkins, Department of Plant Sciences, University of Arizona, Tucson, AZ 85721 Endosperm texture is an important quality trait in maize, as it influences the shipping characteristics of the grain, its susceptibility to insects, the yield of grits from dry milling, energy costs during wet milling, and the baking and digestibility prop… more

Date: March 21, 2003

Creator: Larkins, Brian A.

Item Type: Report

Partner: UNT Libraries Government Documents Department