330 Matching Results

Search Results

Two-Dimensional Gel Electrophoresis of in Vivo and in Vitro Synthesized Proteins, Antigenic Proteins, and Cross-Reactive Antigens in Treponema Pallidum Subsp. Pallidum Nichols Strain and Treponema Phagedenis Biotype Reiter

Description: Two-dimensional electrophoretic protein profiles of in vivo and in vitro propagated T.pallidum subsps. pallidum Nichols strain were analyzed and compared. This comparative analysis revealed two in vitro synthesized, cytoplasmic cylinder-associated polypeptides with molecular masses 29.5 and 34.7 kDa, pI 5.62, and one in vitro "lost" polypeptide with molecular mass 34.7 kDa, pI 5.34. integral membrane proteins of in vitro and in vivo propagated T. pallidum was identified by phase partitioning with the nonionic Triton X-114, and twelve outer membrane-associated, antigenic proteins were identified in western blots probed with pooled human secondary syphilitic sera. The solubilization of the outer membrane of T. pallidum with Triton X-114 were monitored by electron microscopy. Treatment of freshly harvested 35S labeled T. pallidum with 1% Triton X-114 resulted in solubilization of the outer membrane and reduction of the diameter of the treponemes from .14 +/- .02 micrometers to .095 +/- .003 micrometers. Examination of thin sections of untreated organisms showed integrity of outer and cytoplasmic membranes. In contrast, thin sections of Triton X-114-treated trponemes showed integrity of the cytoplasmic membrane but the loss of the outer membrane. The cytoplasmic cylinders generated by detergent treatment retained their periplasmic flagella, as judged by electron microscopy and immunoblotting. Integral membrane proteins of Treponema phagedenis were also identified by phase partitioning with Triton X-114, and sizteen cross-reactive, outer membrane-associated, outer membrane-associated, antigenic polypeptides were identified in western blots probed with pooled human secondary syphilitic sera. The results of this study indicate that tow-dimensional protein profiles of in vivo and in vitro propagated T.pallidum are almost identical except for the differences mentioned. This results also indicate that 1% Triton X-114 selectively solubilizes the outer membrane, and the antigenic hydrophobic proteins present in the detergent phrase are located exclusively in the outer membrane.
Date: May 1989
Creator: Sayahtaheri, Sousan
Partner: UNT Libraries

An Investigation of Differences in Public Library Usage Patterns Between Gifted Adults and Members of the General Public

Description: The purpose of this research was to isolate the variable giftedness in a pipulation and determine whether that variable could be used as a predictor of public library use. The analysis of data indicated that public library use was higher for the general public than for the gifted adults. There was less variation among the factors of age, level of education, and public library use for the gifted adults than for the general public. Books as a resource for information were mentioned by the general adults public more frequently than by the gifted adults. Friends were listed more often as an information resource by the gifted population than by the public. Gifted adults both read and owned more books than did the general public. There was no correlation between amount of reading and number of library visits in either sample. 35 of the general public has a Library Usage Index Value of less than 4, 97 of the gifted adults did. There was almost no difference between the first ten information sources listen by both samples.
Date: December 1989
Creator: Foudray, Rita Catherine Schoch
Partner: UNT Libraries

Interrelational Laboratory Information System for Data Storage and Retrieval

Description: The necessity for a functional user friendly laboratory data management program has become evident as the quantity of information required for modern scientific research has increased to to titanic proportions. The required union of strong computer power, ease of operation, and adaptability have until recently been outside the realm of most research laboratories. Previous systems, in addition to their high cost, are necessarily complex and require software experts in order to effect any changes that the end user might deem necessary. This study examines the Apple Macintosh computer program Hypercard as an interactive laboratory information system that is user-friendly, cost effective, and adaptable to the changing demands within a modern molecular or microbiology.
Date: August 1989
Creator: Gregory, Michael W. (Michael Walter)
Partner: UNT Libraries

The Relationship of Receiving Violence and Perceptions of Self and Partner

Description: The purpose of the present study was to determine whether there are any differences between college students 1) who have received violence 2) who have received threats of physical violence, and 3) who have not received threats or physical violence from their partners. The study examined ways in which these three groups describe their own and their partners' self-esteem and personality. Significant findings indicate that perceptions of one's self esteem and partner's self esteem are affected by the level of violence received. Surprisingly, those who received threats of violence, for both perceptions of self and partners, had the lowest self esteem. No significant relationship was found between vilence and perceptions of personality. Additional findings suggest that those in violent relationships have lower relationship satisfaction that those who receive threats or no violence at all.
Date: August 1989
Creator: Quest, Kathryn
Partner: UNT Libraries

Catalog of the University of North Texas, 1989-1990, Undergraduate

Description: The UNT Undergraduate Bulletin includes information about class offerings as well as general information about the university (academic calendar, admissions and degree requirements, financial information, etc.) about research, and about the colleges and schools on campus. This centennial issue includes additional information about the founding and history of UNT (1890-1990). Index starts on page 248.
Date: June 1989
Creator: University of North Texas
Partner: UNT Libraries

Catalog of the University of North Texas, 1989-1990, Graduate

Description: The UNT Graduate Bulletin includes information about class offerings as well as general information about the university (academic calendar, admissions and degree requirements, financial information, etc.) about research, and about the colleges and schools on campus. This centennial issue includes additional information about the founding and history of UNT (1890-1990). Index starts on page 264.
Date: June 1989
Creator: University of North Texas
Partner: UNT Libraries

Psychic Phenomena Following Near-Death Experiences: An Australian Study

Description: Study examining the incidence of reports of psychic phenomena and associated beliefs both before and after the near-death experience (NDE). The near-death experiencers interviewed reported no more psychic phenomena before the NDE than the general population. There was a statistically significant increase following the NDE in the incidence of 14 of 15 items examined.
Date: Winter 1989
Creator: Sutherland, Cherie
Partner: UNT Libraries

Near-Death Experiences: A Neurophysiological Explanatory Model

Description: Abstract: Prior hypotheses as to the etiology of near-death experiences (NDEs) have been limited to psychiatric explanations or brief discussions of endorphins as causative agents. We present a neurophysiological explanation for NDEs based on their similarities with lysergic acid-, ketamine-, and hypercampnia-induced hallucinations. We believe the core NDE is genetically imprinted and triggered by serotonergic mechanisms.
Date: Autumn 1989
Creator: Morse, Melvin L.; Venecia, David, Jr. & Milstein, Jerrold
Partner: UNT Libraries

Comments on "A Neurobiological Model for Near-Death Experiences"

Description: Article presenting commentary on a neurobiological model proposed by Juan C. Saavedra-Aguilar and Juan S. Gómez-Jeria, which discusses the clinical similarities between temporal lobe seizures and near-death experiences (NDEs). Dr. Morse presents notes regarding his own research with colleagues based on similarities with various neurotransmitters.
Date: Summer 1989
Creator: Morse, Melvin L.
Partner: UNT Libraries

The Physiology of the Tunnel

Description: Article describing several theories to account for the origin of tunnel hallucinations and tunnel experiences near death: (1) the idea of a "real" tunnel; (2) representations of transition; (3) reliving birth memories; (4) imagination; and (5) physiological origins. Three different physiological theories are considered that relate the tunnel form to the structure of the visual cortex.
Date: Autumn 1989
Creator: Blackmore, Susan J. & Troscianko, Tom S.
Partner: UNT Libraries

Unexpected Findings in a Study of Visual Perception During the Naturalistic Near-Death Out-of-Body Experience

Description: Study of visual perception during the naturalistic near-death out-of-body experience (nND OBE), that aspect of the NDE in which the experiencer seems to view normal physical surroundings from a vantage point outside the physical body, which yielded some unexpected findings that contradicted or augmented previous research. Each of these findings is discussed relative to previous research and analyzed in light of the limitations of the current study.
Date: Spring 1989
Creator: Holden, Janice Miner
Partner: UNT Libraries

Clinical Approaches to the Out-of-Body Experience

Description: Abstract: The author reviews aspects of the out-of-body experience (OBE) related to psychic experiences and personality traits, and describes a continuum of experiences of altered mind/body perception, from the prototypical OBE on the healthy end to schizophrenia and organic brain syndromes on the other end. The impact of the OBE on the individual's life is described, with suggestions for a psychoeducational approach to the clinical management of the patient with an OBE to allow maximum growth from the consciousness-expanding effects of the experience.
Date: Autumn 1989
Creator: Twemlow, Stuart W.
Partner: UNT Libraries