Microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography
Description:
High-pressure freezing is the preferred method to prepare thick biological specimens for ultrastructural studies. However, the advantages obtained by this method often prove unattainable for samples that are difficult to handle during the freezing and substitution protocols. Delicate and sparse samples are difficult to manipulate and maintain intact throughout the sequence of freezing, infiltration, embedding, and final orientation for sectioning and subsequent TEM imaging. An established appro…
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Date:
February 13, 2008
Creator:
Auer, Manfred; Triffo, W. J.; Palsdottir, H.; McDonald, K. L.; Inman, J. L.; Bissell, M. J. et al.
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