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Tissue architecture and breast cancer: the role of extracellular matrix and steroid hormones

Description: The changes in tissue architecture that accompany the development of breast cancer have been the focus of investigations aimed at developing new cancer therapeutics. As we learn more about the normal mammary gland, we have begun to understand the complex signaling pathways underlying the dramatic shifts in the structure and function of breast tissue. Integrin-, growth factor-, and steroid hormone-signaling pathways all play an important part in maintaining tissue architecture; disruption of the… more
Date: June 1, 2000
Creator: Hansen, R K & Bissell, M J
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Partner: UNT Libraries Government Documents Department
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The interplay of matrix metalloproteinases, morphogens and growth factors is necessary for branching of mammary epithelial cells

Description: The mammary gland develops its adult form by a process referred to as branching morphogenesis. Many factors have been reported to affect this process. We have used cultured primary mammary epithelial organoids and mammary epithelial cell lines in three-dimensional collagen gels to elucidate which growth factors, matrix metalloproteinases (MMPs) and mammary morphogens interact in branching morphogenesis. Branching stimulated by stromal fibroblasts, epidermal growth factor, fibroblast growth fact… more
Date: March 6, 2002
Creator: Simian, M.; Harail, Y.; Navre, M.; Werb, Z.; Lochter, A. & Bissell, M.J.
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Partner: UNT Libraries Government Documents Department
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CELL SHAPE AND HEXOSE TRANSPORT IN NORMAL AND VIRUS-TRANSFORMED CELLS IN CULTURE

Description: The rate of hexose transport was compared in normal and virus-transformed cells on a monolayer and in suspension. It was shown that: (1) Both trypsin-removed cells and those suspended for an additional day in methyl cellulose had decreased rates of transport and lower available water space when compared with cells on a monolayer. Thus, cell shape affects the overall rate of hexose transport, especially at higher sugar concentrations. (2) Even in suspension, the initial transport rates remained … more
Date: July 1, 1976
Creator: Bissell, M.J.; Farson, D. & Tung, A.S.C.
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Partner: UNT Libraries Government Documents Department
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Expression of Autoactivated Stromelysin-1 in Mammary Glands of Transgenic Mice Leads to a Reactive Stroma During Early Development

Description: Extracellular matrix and extracellular matrix-degrading matrix metalloproteinases play a key role in interactions between the epithelium and the mesenchyme during mammary gland development and disease. In patients with breast cancer, the mammary mesenchyme undergoes a stromal reaction, the etiology of which is unknown. We previously showed that targeting of an autoactivating mutant of the matrix metalloproteinase stromelysin-1 to mammary epithelia of transgenic mice resulted in reduced mammary … more
Date: April 24, 1998
Creator: Thomasset, N.; Lochter, A.; Sympson, C.J.; Lund, L.R.; Williams, D.R.; Behrendtsen, O. et al.
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Partner: UNT Libraries Government Documents Department
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Glucose Metabolite Patterns as Markers of Functional Differentiation in Freshly Isolated and Cultured Mouse Mammary Epithelial Cells

Description: In the mammary gland of nonruminant animals, glucose is utilized in a characteristic and unique way during lactation. We have measured the incorporation of glucose carbon from [U-{sup 14}C] glucose into intermediary metabolites and metabolic products in mammary epithelial cells from virgin, pregnant, and lactating mice and demonstrate that glucose metabolite patterns can be used to recognize stages of differentiated function. For these cells, the rates of synthesis of glycogen and lactose, the … more
Date: June 1, 1980
Creator: Emerman, J. T.; Bartley, J. C. & Bissell, M. J.
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Partner: UNT Libraries Government Documents Department
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Targeting the tumor microenvironment

Description: Despite some notable successes cancer remains, for the most part, a seemingly intractable problem. There is, however, a growing appreciation that targeting the tumor epithelium in isolation is not sufficient as there is an intricate mutually sustaining synergy between the tumor epithelial cells and their surrounding stroma. As the details of this dialogue emerge, new therapeutic targets have been proposed. The FDA has already approved drugs targeting microenvironmental components such as VEGF a… more
Date: November 7, 2006
Creator: Kenny, P. A.; Lee, G. Y. & Bissell, M. J.
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Partner: UNT Libraries Government Documents Department
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GROWTH REGULATION IN RSV INFECTED CHECKEN EMBRYO FIBROBLASTS: THE ROLE OF THE src GENE

Description: The relationship between growth regulation and cell transformation has been studied in many cultured cell lines transformed by a range of oncogenic agents. The main conclusion derived from these investigations is that the nature of the growth regulatory lesion in transformed cells is a function of the agent used to induce transformation. For example, when 3T3 fibroblasts are rendered stationary by serum deprivation, normal cells accumulate in G{sub 1} but SV40 transformed cells are arrested at … more
Date: March 1, 1980
Creator: Parry, G.; Bartholomew, J.C. & Bissell, M.J.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Microaspiration for high-pressure freezing: a new method for ultrastructural preservation of fragile and sparse tissues for TEM and electron tomography

Description: High-pressure freezing is the preferred method to prepare thick biological specimens for ultrastructural studies. However, the advantages obtained by this method often prove unattainable for samples that are difficult to handle during the freezing and substitution protocols. Delicate and sparse samples are difficult to manipulate and maintain intact throughout the sequence of freezing, infiltration, embedding, and final orientation for sectioning and subsequent TEM imaging. An established appro… more
Date: February 13, 2008
Creator: Auer, Manfred; Triffo, W. J.; Palsdottir, H.; McDonald, K. L.; Inman, J. L.; Bissell, M. J. et al.
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Partner: UNT Libraries Government Documents Department
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Tissue phenotype depends on reciprocal interactions between the extracellular matrix and the structural organization of the nucleus

Description: What determines the nuclear organization within a cell and whether this organization itself can impose cellular function within a tissue remains unknown. To explore the relationship between nuclear organization and tissue architecture and function, we used a model of human mammary epithelial cell acinar morphogenesis. When cultured within a reconstituted basement membrane (rBM), HMT-3522 cells form polarized and growth-arrested tissue-like acini with a central lumen and deposit an endogenous BM… more
Date: August 14, 1998
Creator: Lelie'vre, S. A.; Weaver, V. M.; Nickerson, J. A.; Larabell, C. A.; Bhaumik, A.; Petersen, O. W. et al.
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Partner: UNT Libraries Government Documents Department
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Misregulation of Stromelysin-1 in Mouse Mammary Tumor Cells Accompanies Acquisition of Stromelysin-1 dependent Invasive Properties

Description: Stromelysin-1 is a member of the metalloproteinase family of extracellular matrix-degrading enzymes that regulates tissue remodeling. We previously established a transgenic mouse model in which rat stromelysin-1 targeted to the mammary gland augmented expression of endogenous stromelysin-1, disrupted functional differentiation, and induced mammary tumors. A cell line generated from an adenocarcinoma in one of these animals and a previously described mammary tumor cell line generated in culture … more
Date: February 21, 1997
Creator: Lochter, A.; Srebrow, A.; Sympson, C.J.; Terracio, N.; Werb, Z. & Bissell, M.J.
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Partner: UNT Libraries Government Documents Department
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Reciprocal interactions between Beta1-integrin and epidermal growth factor in three-dimensional basement membrane breast cultures: A different perspective in epithelial biology

Description: Anchorage and growth factor independence are cardinal features of the transformed phenotype. Although it is logical that the two pathways must be coregulated in normal tissues to maintain homeostasis, this has not been demonstrated directly. We showed previously that down-modulation of {beta}1-integrin signaling reverted the malignant behavior of a human breast tumor cell line (T4-2) derived from phenotypically normal cells (HMT-3522) and led to growth arrest in a threedimensional (3D) basement… more
Date: September 30, 1998
Creator: Wang, F.; Weaver, V. M.; Petersen, O. W.; Larabell, C. A.; Dedhar, S.; Briand, P. et al.
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Partner: UNT Libraries Government Documents Department
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Functional differentiation and alveolar morphogenesis of primary mammary cultures on reconstituted basement membrane

Description: An essential feature of mammary gland differentiation during pregnancy is the formation of alveoli composed of polarized epithelial cells, which, under the influence of lactogenic hormones, secrete vectorially and sequester milk proteins. Previous culture studies have described either organization of cells polarized towards lumina containing little or no demonstrable tissue-specific protein, or establishment of functional secretory cells exhibiting little or no glandular architecture. In this p… more
Date: February 1, 1989
Creator: BARCELLOS-HOFF, M. H; AGGELER, J.; RAM, T. G & BISSELL, M. J
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Matrix Metalloproteinase Stromelysin-1 Triggers a Cascade of Molecular Alterations that leads to stable epithelial-to-Mesenchymal Conversion and a Premalignant Phenotype in Mammary Epithelial Cells

Description: Matrix metalloproteinases (MMPs) regulate ductal morphogenesis, apoptosis, and neoplastic progression in mammary epithelial cells. To elucidate the direct effects of MMPs on mammary epithelium, we generated functionally normal cells expressing an inducible autoactivating stromelysin-1 (SL-1) transgene. Induction of SL-1 expression resulted in cleavage of E-cadherin, and triggered progressive phenotypic conversion characterized by disappearance of E-cadherin and catenins from cell-cell contacts,… more
Date: August 11, 1997
Creator: Lochter, A.; Galosy, S.; Muschler, J.; Freedman, N.; Werb, Z. & Bissell, M.J.
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Partner: UNT Libraries Government Documents Department
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Suppression of Apoptosis by Basement Membrane Requires three-dimensional Tissue Organization and Withdrawal from the Cell Cycle

Description: The basement membrane (BM) extracellular matrix induces differentiation and suppresses apoptosis in mammary epithelial cells, whereas cells lacking BM lose their differentiated phenotype and undergo apoptosis. Addition of purified BM components, which are known to induce {beta}-casein expression, did not prevent apoptosis, indicating that a more complex BM was necessary. A comparison of culture conditions where apoptosis would or would not occur allowed us to relate inhibition of apoptosis to a… more
Date: December 28, 1995
Creator: Boudreau, N.; Werb, Z. & Bissell, M.J.
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Partner: UNT Libraries Government Documents Department
open access

Epimorphin Functions as a Key Morphoregulator for Mammary Epithelial Cells

Description: Hepatocyte growth factor (HGF) and EGF have been reported to promote branching morphogenesis of mammary epithelial cells. We now show that it is epimorphin that is primarily responsible for this phenomenon. In vivo, epimorphin was detected in the stromal compartment but not in lumenal epithelial cells of the mammary gland; in culture, however, a subpopulation of mammary epithelial cells produced significant amounts of epimorphin. When epimorphin-expressing epithelial cell clones were cultured i… more
Date: October 13, 1997
Creator: Hirai, H.; Lochter, A.; Galosy, S.; Koshida, S.; Niwa, S. & Bissell, M.J.
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Partner: UNT Libraries Government Documents Department
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MCF-10A-NeoST: A New Cell System for Studying Cell-ECM and Cell-Cell Interactions in Breast Cancer

Description: There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers. We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer. NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth… more
Date: August 22, 2001
Creator: Zantek, N. D.; Walker-Daniels, J.; Stewart, J.; Hansen, R. K.; Robinson, D.; Miao, H. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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