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ISOLEUCYL-tRNA-SYNTHETASE A FLUORESCENCE STUDY OP THE BINDINGPROPERTIES OF THE SYNTHETASE FROM ESCHERICHIA COLI

Description: Fluorescence properties of purified isoleucyl-tRNA-synthetase isolated from E. coli B have been studied. No changes in the quantum yield, energy or polarization of the emission were detected in the presence (either individually or in combinations) of the substrates and cofactors required for activation of L-isoleucine. In 2.5 M urea enzyme activity and intrinsic fluorescence intensity (at 340 nm) each decrease with time, showing similar kinetics and rate constants. The rate of this decay is red… more
Date: November 1, 1970
Creator: Penzer, Geoffrey R.; Bennett, Edward L. & Calvin, Melvin.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Preliminary analysis of LOFT Reactor vessel closure. Addendum I, Volume I. Appendix A-3

Description: The purpose of this analysis is to determine if the two piece flat head closure concept presently being considered by INC for the LOFT reactor vessel will meet the structural and fatigue requirements of Article 4 of Section III, ''Nuclear Vessels,'' of the ASME Boiler and Pressure Vessel Code, for the anticipated LOFT operational requirements. The analyses performed include stress analyses based on classical stress analysis methods and proven computer programs, fatigue analyses based on the fat… more
Date: November 1, 1970
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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