8 Matching Results

Search Results

Isolation and Characterization of Malic Enzyme from Ascaris suum

Description: A procedure for the isolation of malic enzyme from muscle tissue of the roundworm Ascaris suum is described. The fractionation method yields relatively large quantities of the enzyme,with a specific activity of fifteen moles of malate converted to pyruvate and carbon dioxide per min per mg at 25ยบ. Homogeneity was established with analytical ultracentrifugation, zone electrophoresis, isoelectric focusing, and rechromatography. The molecular weight of the enzyme was 250,000, and it is dissociated under several conditions into four identical monomers of 64,000 daltons. The enzyme exists as a single electrophoretic form and prefers manganous and NAD over other cations and NADP. Ammonium sulfate competes with manganous for the active site and titration with DTNB yields eight thiol groups per mole. Titration of the first four thiol groups is accompanied by a complete loss in enzyme activity. Equilibrium dialysis, product inhibition, and initial velocity studies suggest a rapid-equilibrium random sequential mechanism for the Ascaris suum malic enzyme. The presence of 1.3 binding sites per subunits was determined for L-ma late. Antisera prepared against A. suum malic enzyme reacted to a small extent with the NAD malic enzymes from two free-living nematodes, Panarellus redivivus and Turbatrix aceti. A correlation coefficient of 0.911 was obtained upon comparing the amino acid composition of A. suum and E. coli malic enzymes. Some sequence homology is predicted between these malic enzymes. The physiological interpretation favors the binding of malate initially, with the subsequent addition of NAD to the enzyme.
Date: December 1972
Creator: Fodge, Douglas W.
Partner: UNT Libraries

Localization of serotonin (5-hydroxytryptamine, 5-HT) with partial purification and characterization of a serotonin binding protein in the intestinal tissue of the nematode Ascaris suum

Description: Muscle, hypodermis and gastrointestinal epithelium from adult female Ascaris lumricoides var. summ were found to contain serotonin based upon glyoxylic acid induced histofluorescence and indirect immunolabeling with an anti-serotonin monoclonal antibody conjugated to protein A-colloidal gold.
Date: May 1989
Creator: Martin, Rex E. (Rex Edward)
Partner: UNT Libraries

Experiment Station Work, [Volume] 56

Description: Bulletin issued by the U. S. Department of Agriculture compiling selected articles from the Agricultural Experiment Stations. This bulletin contains articles on: Incompatibles in Fertilizer Mixtures, Principles of Dry Farming, Methods of Seeding Oats, Rolling v. Harrowing Winter Wheat, Destruction of Eelworms in Soil Pruning, Bean Anthracnose or Pod Spot, Animal Feeds for Farm Stock, Feeding the Pig, Jelly and Jelly Making.
Date: 1910
Creator: United States. Office of Experiment Stations.
Partner: UNT Libraries Government Documents Department

Root-Knot: Its Cause and Control

Description: Report discussing the common plant parasite known as root knot, which is among the most destructive and important of plant diseases. In addition to discussion of the types of plants susceptible to root knot and methods by which the parasite spreads, control and prevention measures are described. "Once established in a field, this pest can be eradicated only by starving it to death by planting for two or three years crops not subject to infection, such as the large and small grains, a few of the leguminous crops, and special resistant varieties of others that have been developed by years of selection and breeding." -- p. ii.
Date: 1923
Creator: Godfrey, G. H. (George Harold), b. 1888
Partner: UNT Libraries Government Documents Department