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UNT Research, Volume 22, 2013

Description: UNT Research magazine includes articles and notes about research at University of North Texas in various academic fields.
Date: 2013
Creator: University of North Texas
Partner: University Relations, Communications & Marketing department for UNT

Use of prior odds for missing persons identifications

Description: This article discusses the use of DNA typing, likelihood ratios, and prior odds for missing persons identifications.
Date: June 27, 2011
Creator: Budowle, Bruce; Ge, Jianye; Chakraborty, Ranajit & Gill-King, Harrell
Item Type: Article
Partner: UNT College of Arts and Sciences

UNT Research, Volume 23, 2014

Description: UNT Research magazine includes articles and notes about research at University of North Texas in various academic fields.
Date: 2014
Creator: University of North Texas
Partner: University Relations, Communications & Marketing department for UNT

Improving Processing Efficiency for Forensic DNA Samples

Description: The goal of this project was to reduce processing time for forensic DNA testing without incurring significant added costs and/or the need for new instrumentation, while still generating high quality profiles. This was accomplished by: 1) extraction normalization using the ChargeSwitch® Forensic DNA Purification Kit such that a small range of DNA concentrations was consistently obtained, eliminating the need for sample quantification and dilution; 2) developing fast PCR protocols for STR primer sets using shorter amplification methods, low volume reactions and non-fast thermal cyclers; and 3) developing a quicker 3130xl Genetic Analyzer detection method using an alternative polymer/array length combination. Extraction normalization was achieved through a reduction in bead quantity, thereby forcing an increase in bead binding efficiency. Four products (AmpliTaq Gold® Fast PCR Master Mix, KAPA2G™ Fast Multiplex PCR Kit, SpeedSTAR™ HS DNA Polymerase and Type-it Microsatellite PCR Kit) were evaluated for low volume (3μl) fast PCR on a 384-well Veriti® thermal cycler with the Identifiler primer set. KAPA2G™ was selected for 3μl fast PCR protocols using PowerPlex 16 HS and Identifiler Plus primer sets (42-51min), as well as 5μl and 6μl Identifiler fast reactions on a 9700 thermal cycler (51-60min). Alternative detection (POP-6™/22cm) achieved 24-28min run times, but with decreased resolution as compared to traditional POP-4®/36cm detection for alleles >200bp; however, 1bp resolution was still obtainable for alleles <300bp. These modifications resulted in robust databasing processes with up to a 37% reduction in processing time for buccal swabs and Buccal DNA Collectors™ using the three primer sets evaluated (3μl fast PCR reactions) and generated high quality STR profiles with ≥90% pass rates.
Date: May 2015
Creator: Connon, Catherine Cupples
Partner: UNT Libraries