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Construction of a Cloning Vector Based upon a Rhizobium Plasmid Origin of Replication and its Application to Genetic Engineering of Rhizobium Strains

Description: Rhizobia are Gram-negative, rod-shaped, soil bacteria with the ability to fix atmospheric nitrogen into ammonia as symbiont bacteroids within nodules of leguminous plant roots. Here, resident Rhizobium plasmids were studied as possible sources of components for the construction of a cloning vector for Rhizobium species.
Date: May 1992
Creator: Jeong, Pyengsoo
Partner: UNT Libraries

Isolation, Characterization and Physiological Studies of Cyanide-Utilizing Bacteria

Description: Ten bacteria capable of growth on the metal-cyano complex, tetracyanonickelate (II) {K2 [Ni(CN)J } (TCN), supplied as the sole nitrogen source, were isolated. Seven isolates were identified as pseudomonads while the remaining three were classified as Klebsiella species. In addition to TCN, all isolates were able to utilize KCN although it was significantly more toxic. The degradation of TCN was most complete when supplied at growth-limiting concentrations, did not occur when ammonia was present, and resulted in the formation of nickel cyanide [Ni(CN)2] as a degradation product.
Date: December 1991
Creator: Silva Avalos, Juan G. (Juan Guillermo)
Partner: UNT Libraries

A Determination of the Effects of Various Concentrations of Sodium Chloride upon the Growth of Three Species of Bacteria

Description: The problem in this investigation is to determine the effects of various concentrations of sodium chloride upon the growth of three species of bacteria. An effort has been made to solve this problem, not only by a study of the relevant literature, but also by laboratory research consisting of cultivation and observation of the three organisms which were arbitrarily chosen for this study.
Date: 1942
Creator: Davis, J. Floyd
Partner: UNT Libraries

The Antibiotic Properties of the Oleoresins of Twenty-Five Common Garden Vegetables

Description: The purpose of this problem is to determine the presence and extent of antibiotic materials as found in the oleoresins of a selected group of garden vegetables. The problem has consisted of, first, the collection and preparation of specimens of twenty-five commonly used garden vegetables; second, the extraction of the oleoresins from these; third, the determination of the inhibitory and other effects of these oleoresins against several strains of selected gram-positive and gram-negative bacteria; and fourth, the evaluation of the potentialities of these oleoresins with regard to their future use as medicinal prophylactics and therapeutics.
Date: 1951
Creator: Ennis, Arthur F.
Partner: UNT Libraries

Studies on the Nutrition of Rhodospirillum Rubrum

Description: The purpose of this study has been to examine the nutritional requirements of Rhodospirillum rubrum and, on the basis of the results, develop a culture medium which could be use to promote more rapid and abundant growth facilitating the laboratory cultivation and observations of the properties so exhibited.
Date: August 1957
Creator: Wentworth, Margaret Earl
Partner: UNT Libraries

Comparative Biochemistry and Evolution of Aspartate Transcarbamoylase from Diverse Bacteria

Description: Aspartate transcarbamoylase (ATCase) catalyzes the first committed step in pyrimidine biosynthesis. Bacterial ATCases are divided into three classes, A, B and C. Class A ATCases are largest at 450-500, are. dodecamers and represented by Pseudomonas ATCase. The overlapping pyrBC' genes encode the Pseudomonases ATCase, which is active only as a 480 kDa dodecamer and requires an inactive pyrC'-encoded DHOase for ATCase activity. ATCase has been studied in two non-pathogenic members of Mycobacterium, M. smegmatis and M. phlei. Their ATCases are dodecamers of molecular weight 480 kDa, composed of six PyrB and six PyrC polypeptides. Unlike the Pseudomonas ATCase, the PyrC polypeptide in these mycobacteria encodes an active DHOase. Moreover, the ATCase: DHOase complex in M. smegmatis is active both as the native 480 kDa and as a 390 kDa complex. The latter lacks two PyrC polypeptides yet retains ATCase activity. The ATCase from M. phlei is similar, except that it is active as the native 480 kDa form but also as 450,410 and 380 kDa forms. These complexes lack one, two, and three PyrC polypeptides, respectively. By contrast,.ATCases from pathogenic mycobacteria are active only at 480 kDa. Mycobacterial ATCases contain active DHOases and accordingly. are placed in class A1 . The class A1 ATCases contain active DHOases while class A2 ATCases contain inactive DHOases. ATCase has also been purified from Burkholderia cepacia and from an E. coli strain in which the cloned pyrB of B. cepacia was expressed. The B. cepacia ATCase has a molecular mass of 550 kDa, with two different polypeptides, PyrB (52 kDa) and PyrC of (39 kDa). The enzyme is active both as the native enzyme at 550 kDa and as smaller molecular forms including 240 kDa and 165 kDa. The ATCase synthesized by the cloned pyrB gene has a molecular weight of 165 kDa composed ...
Date: May 1999
Creator: Hooshdaran, Massoumeh Ziba
Partner: UNT Libraries

The Antibiotic Activities of Some Members of the Cactaceae Family

Description: This problem has been concerned with, first, the collection of sixteen species of plants belonging to Cactaceae family; second, the drying of these and the extraction of the oleoresins thereof; third, the determination of the extent to which these substances inhibit the growth of ten gram-positive and ten gram-negative bacterial organisms; and fourth, a determination of the possible utilization of these extracts a prophylactic or chemotherapeutic agents.
Date: 1951
Creator: Gilmore, Derward E.
Partner: UNT Libraries

A Microbial Survey of Raw Ingredients Used in Finished Products at Kraft Foods Company, Garland, Texas

Description: The purpose of this investigation is to determine through routine checks the number of organisms present in the various raw ingredients used for the preparation of foods. The problem has consisted of, first, a determination of the total bacterial population by numbers; second, a determination of the incidence of colon bacteria in the samples examined; third, a determination of the presence of yeasts and molds; and fourth, an attempt to utilize this information obtained in judging the methods of handling the raw ingredients before they are used in the processing of foods.
Date: 1951
Creator: Langston, Clarence Walter, Jr.
Partner: UNT Libraries

Comparison of Aspartate Transcarbamoylase and Pyrimidine Salvage in Sporosarcina urea, Sprolactobacillus inulinus, Lactobacillus fermentum, and Micrococcus luteus

Description: The enzyme that catalyzes the committed step in pyrimidine biosynthesis, aspartate transcarbamoylase, has been compared in selected endospore-forming organisms and in morphologically similar control organisms. The ATCases and pyrimidine salvage from Sporosarcina ureae, Sporolactobacillus inulinus, Lactobacillus fermentum, and Micrococcus luteus were compared to those of Bacillus subtilis. While the ATCases from Sporosarcina ureae, Sporolactobacillus inulinus, and L. fermentum were found to exhibit characteristics to that of Bacillus with respect to molecular weight and kinetics, M. luteus ATCase was larger at approximately 480 kDa. Furthermore, pyrimidine salvage in Sporosarcina ureae and M. luteus was identical to those of B. subtilis, while pyrimidine salvage of Sporolactobacillus inulinus and L. fermentum resembled that of the pseudomonads.
Date: August 1994
Creator: Barron, Vincent N. (Vincent Neal)
Partner: UNT Libraries

Biofuels from Bacteria, Electricity, and CO2

Description: Advanced Research Projects Agency-Energy project sheet summarizing general information about the Electrofuels program including critical needs, innovation and advantages, impacts, and contact information. This sheet discusses combining ammonia and bacteria to produce liquid fuel as part of the "Biofuels from CO2 Using Ammonia or Iron-Oxidizing Bacteria in Reverse Microbial Fuel Cells" project.
Date: May 10, 2012
Creator: Columbia University
Partner: UNT Libraries Government Documents Department

Biofuels from Solar Energy and Bacteria

Description: Advanced Research Projects Agency-Energy project sheet summarizing general information about the Electrofuels program including critical needs, innovation and advantages, impacts, and contact information. This sheet discusses the conversion of carbon dioxide into liquid fuels as part of the "Electrofuels Via Direct Electron Transfer from Electrodes to Microbes" project.
Date: May 10, 2012
Creator: University of Massachusetts Amherst
Partner: UNT Libraries Government Documents Department

Oxidation of Aromatic Compounds by Bacteria

Description: From Introduction: "The material in this paper is about central paths and how bacterial enzymes manipulate and cleave the aromatic ring with the formation of hydroxylated aromatic ring with the formation
Date: 1962
Creator: Rogoff, Martin H. & Wender, Irving
Partner: UNT Libraries Government Documents Department

Degradation of Complex Carbon Compounds by Marine Actinomycetes

Description: The purpose of this paper is to present a comparative study of marine bacteria, molds and actinomycetes in regard to their ability to degrade certain pure and mixed complex compounds possibly occurring in the lagoon waste traps of the Texas Gulf Coast. This comparison was made using a differential oxygen uptake as the index of specific compound utilization.
Date: August 1959
Creator: Willingham, Charles Allen
Partner: UNT Libraries

Morphological and Physiological Changes in Micrococcus Pyogenes Var. Aureus during Development of its Resistance to Terramycin

Description: The problem in this investigation consists of, first, the procurement of several strains of Micrococcus pyogenes var. aureus; second, the comparison of the degree and rate of development of resistance of these organisms to terramycin; and, third, to study the morphological and physiological changes which occur during the development of resistance.
Date: August 1959
Creator: Watson, Gerald T.
Partner: UNT Libraries

Soybean diseases.

Description: Discusses a multitude of diseases that affect soybean crops, the adaptability of available resistant varieties, and a small amount of control measures.
Date: March 1955
Creator: Johnson, Howard W. (Howard Wilfred), 1901-; Chamberlain, Donald W. (Donald William), 1905- & Lehman, Samuel George, 1887-
Partner: UNT Libraries Government Documents Department

Role of α-Keto Acids In Cyanide Detoxification and Assimilation by Pseudomonas Bacteria

Description: Cyanide was rapidly removed when added to culture supernatants of seven different Pseudomonas. The ability to remove cyanide was correlated with the accumulation of α-keto acids (pyruvate and α-ketoglutarate). These compounds react with cyanide forming less toxic cyanohydrins, thus conferring a mechanism for bacterial cyanide tolerance. When added to growth media the α-keto acids were shown also to serve as effective cyanide antagonists. While all bacteria tested accumulated α-keto acids, only those capable of utilizing cyanide as a nutritional nitrogen source were able to metabolize cyanohydrins. In P. fluorescens NCIMB 11764, the same enzyme (cyanide oxygenase) shown previously to be involved in cyanide metabolism appears responsible for cyanohydrin transformation. Keto acid excretion is believed to represent a new mechanism of bacterial cyanide detoxification with further enzymatic metabolism of the cyanohydrins helping to explain how cyanide can satisfy the nitrogen requirement in cyanide-utilizing bacteria.
Date: December 1998
Creator: Pan, Guangliang
Partner: UNT Libraries