Effects of Methanol, Atrazine, and Copper on the Ultrastructure of Pseudokirchneriella Subcapitata (Selenastrum Capricornutum).
Description: The toxicity of methanol, atrazine, and copper to Pseudokirchneriella subcapitata (Korshikov) Hindák historically referred to as Selenastrum capricornutum Printz were determined following 96 hrs growth in a modified Goram's growth media. Methanol and atrazine inhibited fluorescence readings in the cultures by 50% (IC50) at concentrations of 2% and 82 µg/l respectively. These toxicity values compared favorably to other published reports. The IC50 for copper was 160 µg/l which is substantially higher than reported values. This is understandable because of the high chelating capacity of Goram's media. The use of stereologically derived relative volume in the chloroplasts, mitochondria, lipid bodies, phosphate bodies, and nucleus was investigated to determine if it could be used as a sensitive endpoint in toxicity tests. The volume fractions for the chloroplasts and mitochondria were normally distributed in control cells while the nuclei, phosphate bodies, and lipid bodies were not. The chloroplasts were the most dominate organelle occupying a mean relative volume of 46% and mitochondria occupied a mean relative volume of 3%. The nucleus and phosphate bodies occupied a median relative volume of 7% and 2% respectively. The lipid bodies were rare in section profile and no meaningful median relative volume could be calculated. Up to the 82nd percentile of sectioned profiles contained no recognizable lipid bodies. The use of relative volume was not a sensitive endpoint for use in toxicity tests. No significant differences in relative volume could be detected in the nucleus or phosphate bodies following any treatment. Limited differences were detected in the mitochondria, chloroplasts, and lipid bodies. The only significant differences that appear to be biologically significant occurred in methanol treated cells where an increase in the lipid bodies' relative volume was apparently concentration dependent. Significant differences in the relative volume of mitochondria and chloroplasts do not appear to be biologically significant.
Date: May 2004
Creator: Garrett, David C.
Partner: UNT Libraries