4 Matching Results

Search Results

Regulation of Pyridine Nucleotide Metabolism in Saccharomyces cerevisiae

Description: The levels of total nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP), and their redox states were determined as the function of growth in S. cerevisiae. Cells growing in a medium containing 0.8% glucose exhibit two phases of exponential growth, utilizing glucose and ethanol, respectively. The NAD pool is 50% reduced during both stages of growth while the NADP pool is 67% reduced in glucose growth and 48% reduced in ethanol growth. The NAD/NADP ratio is constant during growth on glucose and a two-fold increase in the NAD/NADP ratio occurs upon exhaustion of glucose. The increased ratio is maintained during growth on ethanol. This alteration in the regulation of the relative levels of NAD and NADP may be due to a change in the regulation of NAD kinase and/or NADP phosphatase activities. These changes may be related to the redox state of the NADP pool.
Date: May 1976
Creator: Ting, Haung-yu
Partner: UNT Libraries

Screening of steroids from plants using Saccharomyces cerevisiae as a host system

Description: The reconstituted steroid transcription unit in Saccharomyces cerevisae was used to screen for steroids in cell extracts prepared from 24 species of plants representing 16 families. Extracts from 7 species representing 5 families were able to activate both estrogen and progesterone receptors expressed in yeast.
Date: August 1992
Creator: Maier, Camelia G. A. (Camelia Gabriela-Anca)
Partner: UNT Libraries