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The Reproductive Consequences of Carriers of Methylenebisacrylamide-Induced Balanced Reciprocal Translocations in Mus Musculus

Description: N,N'-methylenebisacrylamide (MBA) was studied because of its effectiveness in inducing heritable translocations in germ cells of male mice. The health impact of translocations was studied through anatomical analysis of the progeny of semisterile translocation carriers. As expected, the semisterility of translocation carriers resulted primarily from embryonic death during periimplantation stages due to unbalanced chromosome sperm segregants. Among conceptuses that survived to mid- and late-gesta… more
Date: May 1989
Creator: Kile, Joanna L. (Joanna Le)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Short Term Effects of External Electric Fields on Electrical Activity of the Pineal Gland in Rats

Description: The effects of short term exposure (5 minutes) to EEFs at relatively high dosages (10, 25, 39, kV/m) on the electrical activity in rat pineal glands was studied. Daytime and nighttime recordings were taken from an implanted microelectrode in the gland. The data show that (1) both the activity and frequency were enhanced when the animals were exposed to EEFs at 39 kV/m continuously and discontinuously; (2) the later condition yielded a sustained increase (36%) whereas the former a brief (10 sec)… more
Date: May 1996
Creator: Vu, Hung Quoc
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Novel Role of Trypsin in Zebrafish

Description: It has been shown previously in our laboratory that zebrafish produce trypsin from their gills when they are under stress, and this trypsin is involved in thrombocyte activation via PAR2 during gill bleeding. In this study, I investigated another role of the trypsin that is secreted from zebrafish. This investigation has demonstrated a novel role of trypsin in zebrafish. Not only did this investigation demonstrate the role of trypsin in zebrafish behavior, but also it showed that PAR2 might be … more
Date: May 2013
Creator: Alsrhani, Abdullah Falleh
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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College Freshman Biology Two Semester Course: Integrating Deep Processing Teaching Techniques

Description: Development of a college level freshman biology course was undertaken in response to government reports that American students have fallen behind students of other countries in the area of the sciences. Teaching strategies were investigated to accomplish two objectives, to define essential academic material to include in the course and to investigate teaching techniques that would increase deep processing of the information. An active process that consisted of applying the cognitive information… more
Date: May 2002
Creator: Blevins, Mary Jean
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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The Use of Genetic Polymorphisms and Discriminant Analysis in Evaluating Genetic Polymorphisms as a Predictor of Population

Description: Discriminant analysis is a procedure for identifying the relationships between qualitative criterion variables and quantitative predictor variables. Data bases of genetic polymorphisms are currently available that group such polymorphisms by ethnic origin or nationality. Such information could be useful to entities that base financial determinations upon predictions of disease or to medical researchers who wish to target prevention and treatment to population groups. While the use of genetic in… more
Date: May 2002
Creator: Howell, Bruce F.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Effects of Daily Oral Injections of Quercetin on Implanted Colon-25 Tumor Growth in Balb-C Mice

Description: The effects of three oral dosages (0.4 mg, 0.8 mg, and 1.6 mg) of quercetin on Colon-25 tumors implanted in Balb-c mice were studied. The data in this study show that: (1) certain dosages of quercetin in alcohol solutions, reduces the weight, and size of implanted Colon-25 tumors in Balb-c mice, (2) these same dosages of quercetin all produce a profound neutrophilia combined with a significant lymphopenia at day 20 post-implantation, and (3) there was relatively little evidence of histological … more
Date: May 2000
Creator: Hayashi, Adam
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Mutagenized HLA DNA Constructs: Tools for Validating Molecular HLA Typing Methodologies

Description: This study describes the development and validation of mutagenized cloned DNA constructs, which correspond to the polymorphic regions of the class II region of the HLA complex. The constructs were used to verify the allelic specificity of primers and probes in polymerase chain reaction (PCR)-based HLA typing assays such as Sequence Specific Primers (SSP) and Sequence Specific Oligonucleotide Probes (SSOP). The constructs consisted of the entire polymorphic region of exon 2 of class II HLA allel… more
Date: May 1999
Creator: Schulte, Kathleen Q.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries

Animal Contribution to Human Medicine

Description: The use of animal models in research has led to a fierce debate between animal rights activists and scientists. The former claim that little useful information is gained from animal studies and the suffering of animals does not preclude any treatments which may be used to treat human illnesses. Yet, research scientists claim that in vivo animal models are of absolute necessity to developing treatments and cures to disease. To determine the necessity of animal use, one must examine the models c… more
Access: Restricted to UNT Community Members. Login required if off-campus.
Date: May 2001
Creator: Kvernes, Kayce
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

A Two Semester Life Science Syllabus for Use in Texas Public Schools with Seventh Grade Students

Description: The problem of using a state adopted textbook written to apply to a large body of students with varying interests and needs was overcome by using a detailed syllabus that arranged course content in a meaningful sequence that appealed to student interest. The outlined syllabus prepared a two semester life science curriculum to be used by the teacher to guide lesson planning. Both semesters were divided into three units each. Materials included in the syllabus were given to actual student groups … more
Date: May 1995
Creator: Edwards, Gail G. (Gail Graham)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Characterization of Aspartate Transcarbamoylase and Dihydroorotase in Moraxella Catarrhalis

Description: Bacterial aspartate transcarbamoylases (ATCase's) are divided into three classes that correspond to taxonomic relationships within the bacteria. The opportunistic pathogen Moraxeila catarrhalis has undergone several reclassifications based on traditional microbiological criteria. The previously uncharacterized ATCase from M. catarrhalis was purified to homogeneity and its chemical properties characterized. The ATCase from M. catarrhalis is a class C ATCase with an apparent molecular mass of 480… more
Date: May 1998
Creator: Fowler, Michael A. (Michael Allen), 1961-
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Anatomy and Physiology Syllabus for Community Colleges

Description: This syllabus includes both lecture notes and laboratory activities for a two-semester anatomy and physiology community college course. The syllabus is based on a 16-week semester that is comprised of a three-hour lecture section and a one-hour laboratory class each week. Both the lecture course and laboratory are necessary to fulfill the requirement for anatomy and physiology. Laboratory activities coincide with lectures to enhance understanding of each topic by providing visual and hands-on e… more
Date: May 2000
Creator: Schulz, Leslie
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Characterization of the OCT Plasmid-Encoded Mercury Resistance Genetic Locus in Pseudomonas putida

Description: A 17.1 Kb genetic element encoding for mercury resistance (OCT-Hg^r) was shown to translocate from its original location on the OCT plasmid to the resistance plasmid, RPl, in Pseudomonas putida. Analysis of RPl-Hg^r recombinant plasmids revealed that insertion of mercury resistance genes into RPl could occur at a variety of sites, with all recombinants having common EcoRI restriction fragments of 9.4, 3.8, 2.3, and 1.6 Kb, derived from the insertion. Hybridization analysis suggested the existen… more
Date: May 1989
Creator: Armbruster, Steven C. (Steven Christopher)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

An Interpretation of Archaic Medical Treatises

Description: Ancient peoples did not distinguish between philosophy, religion, and science. Scientific truth did not exist apart from divine truth. Any new idea, finding, or theory was assimilated into a monolithic mythological structure. This is one of the causes of the underestimation of ancient science: it is always packaged in a myth - the method of preserving information in an oral culture. The mythological medium allowed the preservation and dissemination of hard-won, empirical, scientific knowledge t… more
Date: May 1993
Creator: Wagers, William D. (William Delbert)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Advanced Molecular and Microbial Techniques: a Complete Laboratory Notebook

Description: The purpose of this project is to produce a complete and thorough notebook that may be used to supplement laboratory coursework. Its intent is to be used primarily by the students to aid them in understanding background information and the proper laboratory procedures involved in various types of experiments. The laboratory notebook is a summation of all the experiments and procedures used in the six-credit hour Advanced Microbial and Molecular Biology (BIOL 5160) course offered during the summ… more
Date: May 1998
Creator: Brito-Rodriquez, Carmen Lydia
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Regulation of Escherichia coli pyrBI Gene Expression in Pseudomonas fluorescens

Description: Pseudomonas fluorescens does not appear to regulate the enzymes of de novo pyrimidine biosynthesis at the level of gene expression. Little or no apparent repression of pyr gene expression is observed upon addition of exogenous pyrimidines to the growth medium. The Escherichia coli pyrBI genes for aspartate transcarbamoylase (ATCase) were sized down and cloned into the broad host range plasmid, pKT230. Upon introduction into a P.fluorescenspyrB mutant strain, ATCase showed repression in response… more
Date: May 1995
Creator: Shen, Weiping
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Identification and Characterization of the Pyrimidine Biosynthetic Operon in Streptomyces griseus

Description: To further understand the ATCase/DHOase bifunctional complex formed in Streptomyces, the genes encoding these and other pyrimidine enzymes were identified and characterized. Polymerase chain reaction (PCR) was utilized in this effort. Primers were constructed by selecting conserved regions of pyrimidine genes from known gene and protein sequences of a wide variety of organisms. These sequences were then optimized to Streptomyces codon usage. PCR products were obtained from internal sites within… more
Date: May 1998
Creator: Hooten, Jody J. (Jody Jeran)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Nucleotide Sequence of a Bovine Arginine Transfer RNA Gene

Description: A single plaque-pure lambda clone designated λBA84 that hybridized to a ˆ32P-labeled bovine arginine tRNA was isolated from a bovine genomic library harbored in a lambda bacteriophage vector. A 2.3-kilobase segment of this clone was found to contain an arginine transfer RNAccg gene by Southern blot hybridization analysis and dideoxyribonucleotide DNA sequencing. This gene contains the characteristic RNA polymerase III split promoter sequence found in all eukaryotic tRNAs and a potential RNA pol… more
Date: May 1996
Creator: Eubanks, Aleida C. (Aleida Christine)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Virulence Factor Production in PyrE Mutants of Pseudomonas Aeruginosa

Description: It has been shown previously in our lab that mutations in the pyrimidine pathway reduced the ability of Pseudomonas aeruginosa to produce virulence factors. Knockout mutations in pyrB, pyrC and pyrD genes of the pyrimidine pathway showed that virulence factor production was decreased. Pyoverdin, pyocyanin, hemolysin, iron chelation, motility, and adherence are all considered virulence factors. Here I further investigate the effects of mutations in the pyrimidine pathway by studying a pyrE mutan… more
Date: May 2010
Creator: Niazy, Abdurahman
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Bacterial Cyanide Assimilation: Pterin Cofactor and Enzymatic Requirements for Substrate Oxidation

Description: Utilization of cyanide as the sole nitrogen source by Pseudomonas fluorescens NCIMB 11764 (Pf11764) occurs via oxidative conversion to carbon dioxide and ammonia, the latter satisfying the nitrogen requirement. Substrate attack is initiated oxygenolytically by an enzyme referred to as cyanide oxygenase (CNO), which exhibits properties of a pterin-dependent hydroxylase. The pterin requirement for Pf11764 CNO was satisfied by supplying either the fully (tetrahydro) or partially (dihydro) reduced … more
Date: May 2004
Creator: Dolghih, Elena
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Rapid Isolation and Purification of Plasmid DNA Using High Performance Liquid Chromatography

Description: High Performance Liquid Chromatography (HPLC) has been employed as an analytical tool for the purification and separation of nucleic acids. A Nucleogen DEAE 4000-10 weak anion exchange column, prepacked with modified silica gels, was used to purify and separate a number of Escherichia coli plasmids. Plasmid DNAs were extracted by the alkaline lysis method. The cleared lysate was injected directly onto the Nucleogen column, and the peaks were collected, desalted and analysed by gel electrophores… more
Date: May 1987
Creator: Nam, Kiebang
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries

Inherent Problems Associated with the Identification of Genes Responsible for Allowing B. cepacia to Adhere to Human Lung Carcinoma A549 Cells

Description: In this project a bacteria's ability to bind to human lung tissue was investigated. To carry out this study Pseudomonas aeruginosa, Eschericia coli and Burkholderia cepacia were used. B. cepacia served as the bacterium of interest. Isolating the gene which confers upon this bacterium the ability to bind to lung tissue was the main objective of this study. P. aeruginosa has been identified as being the bacteria most responsible for causing serious lung infections that can result in cystic f… more
Access: Restricted to UNT Community Members. Login required if off-campus.
Date: May 2001
Creator: Nesty, Gilda S.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

The Teratogenic Effects of Nocodazole and Acrylamide in Mus Musculus

Description: In two separate experiments, weight adjusted doses of nocodazole and acrylamide were injected intraperitoneally at various time intervals into twelve week old female mice. Within the nocodazole experiment, the doses were injected at varying time intervals before and after mating. On day seventeen of gestation, the female mice were sacrificed and their uterine contents examined. Nocodazole induced a significant increase in reproductive pathology per total implants when administered one hour aft… more
Date: May 1989
Creator: Oliva, Jean L. (Jean Louise)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Effector Response of the Aspartate Transcarbamoylase From Wild Type Pseudomonas Putida and a Mutant with 11 Amino Acids Deleted at the N-terminus of PyrB.

Description: Like its enteric counterpart, aspartate transcarbamoylase (ATCase) from Pseudomonas putida is a dodecamer of two different polypeptides. Unlike the enterics, the Pseudomonas ATCase lacks regulatory polypeptides but employs instead inactive dihydroorotases for an active dodecamer. Previous work showed that PyrB contains not only the active site but also the effector binding sites for ATP, UTP and CTP at its N-terminus. In this work, 11 amino acids were deleted from the N-terminus of PyrB and t… more
Date: May 2002
Creator: Asfour, Hani
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Construction of a Physical Map of Moraxella (Branhamella) catarrhalis Strain ATCC25238

Description: In order to gain a better understanding of this microorganismand its role in human pathogenesis, a physical map of Moraxella catarrhalis type strain ATCC25238 was constructed using pulsed field gel electrophoresis (PFGE) in combination with Southern hybridization techniques. Restriction endonucleases Not I, Rsr II, and Sma I were used to digest the chromosomal DNA. An overlapping circular map was generated by cross-hybridization of isolated radiolabeled fragments of Moraxella catarrhalis genomi… more
Date: May 1999
Creator: Nguyen, Kim Thuy
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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