7 Matching Results

Search Results

Callus Development and Organogenesis in Cultured Explants of Cowpea (Vigna unguiculata (L.) Walp

Description: Cowpea, Vigna unguiculata (L.) Walp is an excellent source of protein, vitamins and minerals and a major food crop many parts of Africa. Optimal production levels are hampered by insect pests and diseases. Biotechnological techniques such as tissue culture and genetic engineering can aid in the development of varieties with resistance to insect pests and diseases. The objective of this study was to investigate conditions necessary for the development of a reproducible tissue culture system that can be applied to regenerate transformed cells from culture. The in vitro manipulation of cowpea using Murashige and Skoog (MS) medium, auxins and cytokinins resulted in the formation of callus and rhizogenesis. Calli that were formed were separated into six classes based on color and texture. Yellowish friable callus, yellowish compact, soft yellowish callus and green and white were composed of largely vacuolated cells and were non-regenerative. Friable green callus was the most prevalent callus type and could form of roots in some hormone combinations. Green spots were formed on hard compact green callus. The green spots became nodular, forming root primordia and ultimately giving rise to roots. None of the six calli types gave rise to the formation of shoots. Embryogenic callus was induced from cowpea explants cultured on MS medium supplemented with dicamba and picloram. Embryogenic suspension cultures were initiated from callus induced on MS supplemented with 3.0 mg/L dicamba or picloram and conditions for maintenance of embryogenic suspension cultures were evaluated. Somatic embryos were formed in suspension cultures. Attempts to convert and germinate the somatic embryos resulted in the formation of callus or formation of appendages on the somatic embryos or in the death of the embryos. The appendages formed roots on prolonged culture. Further research is needed to determine appropriate optimal conditions for embryo conversion and germination and ultimately plant ...
Access: This item is restricted to UNT Community Members. Login required if off-campus.
Date: December 2004
Creator: Omwenga, George Isanda
Partner: UNT Libraries

Cowpeas: Culture and Varieties

Description: Report discussing the cowpea, a leguminous crop often grown in the Southern United States. Topics discussed include its several varieties, fertilizers, methods of sowing, and diseases.
Date: 1920
Creator: Morse, W. J. (William Joseph), b. 1884
Partner: UNT Libraries Government Documents Department

Cowpeas: Utilization

Description: Report discussing common uses of the cowpea and methods of cultivation which improve crop yields for these purposes. The cowpea may be used for seed, straw, hay, pasture, or soil improvement.
Date: 1920
Creator: Morse, W. J. (William Joseph), b. 1884
Partner: UNT Libraries Government Documents Department

Cowpeas

Description: "The cowpea is the most valuable legume for the Southern States and its use would be much more extensive were it not for the relatively high price of the seed, most of which is still picked by hand. Particular attention is therefore given to the matter of harvesting seed by machinery now in very successful use in several communities. These methods are so far perfected that the cowpea seed crop should receive much greater attention in favorable localities." -- p. 5. This bulletin also discusses the use of cowpeas for hay, seed mixtures of cowpeas and other crops, the nutritional value of cowpeas in animal feeds, growing practices, and the several different varieties of cowpea.
Date: 1908
Creator: Nielsen, H. T. (Harold T.)
Partner: UNT Libraries Government Documents Department

Cowpeas

Description: Report promoting the cultivation of the cowpea as a forage plant because of it adaptability to a wide variety of soils and agricultural conditions.
Date: 1899
Creator: Smith, Jared G. (Jared Gage), 1866-1957
Partner: UNT Libraries Government Documents Department

Use of Corn, Kafir, and Cowpeas in the Home

Description: "This bulletin summarizes the results of some tests of the nutritive value of [of Indian corn, Kafir corn, and cowpeas] and ways of preparing them for the table." -- title page. Includes recipes.
Date: 1913
Creator: Langworthy, C. F. (Charles Ford), 1864-1932 & Hunt, Caroline Louisa, 1865-1927
Partner: UNT Libraries Government Documents Department