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Analysis of the Genome of a Korean Isolate of the Pieris rapae Granulovirus Enabled by Its Separation from Total Host Genomic DNA by Pulse-Field Electrophoresis

Description: Article on an analysis of the genome of a Korean isolate of the Pieris rapae granulovirus enabled by its separation from total host genomic DNA by pulse-field electrophoresis.
Date: December 31, 2013
Creator: Jo, Yong Hun; Patnaik, Bharat Bhusan; Kang, Se Won; Chae, Sung-Hwa; Oh, Seunghan; Kim, Dong Hyun et al.
Item Type: Refine your search to only Article
Partner: UNT College of Arts and Sciences
open access

The morphologies of breast cancer cell lines in three-dimensionalassays correlate with their profiles of gene expression

Description: 3D cell cultures are rapidly becoming the method of choice for the physiologically relevant modeling of many aspects of non-malignant and malignant cell behavior ex vivo. Nevertheless, only a limited number of distinct cell types have been evaluated in this assay to date. Here we report the first large scale comparison of the transcriptional profiles and 3D cell culture phenotypes of a substantial panel of human breast cancer cell lines. Each cell line adopts a colony morphology of one of four … more
Date: January 31, 2007
Creator: Kenny, Paraic A.; Lee, Genee Y.; Myers, Connie A.; Neve, RichardM.; Semeiks, Jeremy R.; Spellman, Paul T. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

PointCloudExplore 2: Visual exploration of 3D gene expression

Description: To better understand how developmental regulatory networks are defined inthe genome sequence, the Berkeley Drosophila Transcription Network Project (BDNTP)has developed a suite of methods to describe 3D gene expression data, i.e.,the output of the network at cellular resolution for multiple time points. To allow researchersto explore these novel data sets we have developed PointCloudXplore (PCX).In PCX we have linked physical and information visualization views via the concept ofbrushing (cell … more
Date: March 31, 2008
Creator: International Research Training Group Visualization of Large and Unstructured Data Sets, University of Kaiserslautern, Germany; Institute for Data Analysis and Visualization, University of California, Davis, CA; Computational Research Division, Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA; Genomics Division, LBNL; Computer Science Department, University of California, Irvine, CA; Computer Science Division,University of California, Berkeley, CA et al.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Process Design for the Biocatalysis of Value-Added Chemicals from Carbon Dioxide

Description: This report describes results toward developing a process to sequester CO{sub 2} centered on the enzymes PEP carboxylase and pyruvate carboxylase. The process involves the use of bacteria to convert CO{sub 2} and glucose as a co-substrate and generates succinic acid as a commodity chemical product. The study reports on strain development and process development. In the area of strain development, knockouts in genes which divert carbon from the enzymatic steps involved in CO{sub 2} consumption w… more
Date: July 31, 2007
Creator: Eiteman, Mark
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Molecular dissection of the roles of the SOD genes in mammalian response to low dose irradiation

Description: It has been long recognized that a significant fraction of the radiation-induced genetic damage to cells are caused by secondary oxidative species. Internal cellular defense systems against oxidative stress play significant roles in countering genetic damage induced by ionizing radiation. The role of the detoxifying enzymes may be even more prominent in the case of low-dose, low-LET irradiation, as the majority of genetic damage may be caused by secondary oxidative species. In this study we hav… more
Date: August 31, 2006
Creator: Chuang, Eric Y.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Mechanisms of Low Dose Radiation-induced T helper Cell Function

Description: Exposure to radiation above levels normally encountered on Earth can occur during wartime, accidents such as those at Three Mile Island and Chernobyl, and detonation of “dirty bombs” by terrorists. Relatively high levels of radiation exposure can also occur in certain occupations (low-level waste sites, nuclear power plants, nuclear medicine facilities, airline industry, and space agencies). Depression or dysfunction of the highly radiosensitive cells of the immune system can lead to serious co… more
Date: October 31, 2008
Creator: Gridley, Daila S.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Characterization of the organic-sulfur-degrading enzymes. Technical report, September 1, 1991--November 30, 1991

Description: The objective of the proposed research is to first characterize the enzymes involved in degrading organic sulfur in coal from some of the well-characterized organic sulfur-degrading strains. After the enzymes have been successfully purified, the genes encoding these enzymes will be cloned and the suitability of these microorganisms for recombinant manipulation will also be studied. Initially, the enzymes involved in the removal of organic sulfur from coal and from model compounds in two particu… more
Date: December 31, 1991
Creator: Ho, N. W. Y.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Mechanisms and genetic control of interspecific crossing barriers in lycopersicon. Progress report, First year, August 1, 1992

Description: The goal of this program is to use Lycopersica esculentum and L. pennellii as a model system to study the interspecific reproductive barriers unilateral incongruity (UI), hybrid breakdown and interspecific aberrant ratio syndrome (IARS). Specifically we seek to determine the functional basis of UI including the timing of the failure of incongruous crosses, the developmental step(s) interrupted by UI, the tissue and genomes involved in UI.
Date: December 31, 1992
Creator: Mutschler, M. A. & McCormick, S.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Large scale solubilization of coal and bioconversion to utilizable energy. Quarterly technical progress report, September--December 1993

Description: In order to develop a system for a large scale coal solubilization and its bioconversion to utilizable fuel, we plan to clone the genes encoding Neurospora protein that facilitate depolymerization of coal. We also plan to use desulfurizing bacteria to remove the sulfur in situ and use other microorganisms to convert biosolubilized coal into utilizable energy following an approach utilizing several microorganisms (Faison, 1991). In addition the product of coal solubilized by fungus will be chara… more
Date: December 31, 1993
Creator: Mishra, N. C.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Agave: a biofuel feedstock for arid and semi-arid environments

Description: Efficient production of plant-based, lignocellulosic biofuels relies upon continued improvement of existing biofuel feedstock species, as well as the introduction of newfeedstocks capable of growing on marginal lands to avoid conflicts with existing food production and minimize use of water and nitrogen resources. To this end, specieswithin the plant genus Agave have recently been proposed as new biofuel feedstocks. Many Agave species are adapted to hot and arid environments generally unsuitabl… more
Date: May 31, 2011
Creator: Gross, Stephen; Martin, Jeffrey; Simpson, June; Wang, Zhong & Visel, Axel
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Biosurfactants and increased bioavailability of sorbed organic contaminants: Measurements using a biosensor

Description: Bioremediation of sites contaminated with hydrophobic materials that sorb onto the soil matrix is very difficult due to reduced microbial (bio)availability. Following biosurfactant addition, we have measured an increase in contaminant bioavailability by using a lux biosensor. Direct microbial bioavailability was determined by using a genetically engineered microbial bioreporter strain of Pseudomonas putida. This strain was engineered so the lux genes, which code for light production, are transc… more
Date: December 31, 1993
Creator: Strong-Gunderson, J. M.; Palumbo, A. V.; Applegate, B. & Saylor, G. S.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Thiophene metabolism by E. coli. Final technical report, September 15, 1987--December 31, 1991

Description: The objective for this project was to continue the genetic analysis of the thiophene oxidation system, in particular: 1. characterization of the biochemical pathway for thiophene oxidation, 2. identification and mapping of any further genes involved in thiophene degradation, 3. analysis of how the thd genes are regulated, and 4. cloning and sequencing of at least some of the thd genes.
Date: December 31, 1991
Creator: Clark, D. P.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

[An homologous recombination strategy to directly clone mammalian telemeres]. Progress report

Description: We have pursued three goals over the past year. The first involved determining whether the HARY vector could be used for homologous integration in the human genome. The second was to ascertain whether inserted sequences could be amplified in preference to the endogenous DHFR genes. The third was to determine if the HARY insertion could provide an anchor point for long range restriction mapping. The progress in each goal is described.
Date: December 31, 1992
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Biochemistry and genetics of autotrophy in Methanococcus

Description: The project investigated fundamental aspects of carbon metabolism and genetics in the methane-producing archaeon Methanococcus maripaludis. The project yielded 23 peer-reviewed publications and five reviews from 1997-2007. PDFs of the peer-reviewed publications are included in the next section. Some papers of special interest are listed below. The pathway of pyruvate biosynthesis was elucidated by a combination of biochemical and physiological studies. This work characterized the very oxygen se… more
Date: March 31, 2009
Creator: Whitman, William B.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Functional Analysis of Arabidopsis Sucrose Transporters

Description: Sucrose is the main photosynthetic product that is transported in the vasculature of plants. The long-distance transport of carbohydrates is required to support the growth and development of net-importing (sink) tissues such as fruit, seeds and roots. This project is focused on understanding the transport mechanism sucrose transporters (SUTs). These are proton-coupled sucrose uptake transporters (membrane proteins) that are required for transport of sucrose in the vasculature and uptake into si… more
Date: March 31, 2009
Creator: Ward, John M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Comparative Genomics of Regulation of Fatty Acid and Branched-chain Amino Acid Utilization in Proteobacteria

Description: Bacteria can use branched-chain amino acids (ILV, i.e. isoleucine, leucine, valine) and fatty acids (FA) as sole carbon and energy sources convering ILV into acetyl-CoA, propanoyl-CoA and propionyl-CoA, respectively. In this work, we used the comparative genomic approach to identify candidate transcriptional factors and DNA motifs that control ILV and FA utilization pathways in proteobacteria. The metabolic regulons were characterized based on the identification and comparison of candidate tran… more
Date: October 31, 2008
Creator: Kazakov, Alexey E.; Rodionov, Dmitry A.; Arkin, Adam Paul; Dubchak, Inna; Gelfand, Mikhail S. & Alm, Eric
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Hemicellulolytic organisms in the particle-associated microbiota of the hoatzin crop

Description: The hoatzin (Opisthocomus hoazin) is a South American herbivorous bird, that has an enlarged crop analogous to the rumen, where foregut microbes degrade the otherwise indigestible plant materials, providing energy to the host. The crop harbors an impressive array of microorganisms with potentially novel cellulolytic enzymes. Thie study describes the composition ofthe particle-associated microbiota in the hoatzin crop, combining a survey of 16S rRNA genes in 7 adult birds and metagenome sequenci… more
Date: May 31, 2011
Creator: Godoy-Vitorino, Filipa; Malfatti, Stephanie; Garcia-Amado, Maria A.; Dominguez-Bello, Maria Gloria; Hugenholtz, Phillip & Tringe, Susannah
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Process Design for the Biocatalysis of Value-Added Chemicals from Carbon Dioxide

Description: This report describes results toward developing a process to sequester CO{sub 2} centered on the enzyme pyruvate carboxylase. The process involves the use of bacteria to convert CO{sub 2} and glucose as a co-substrate and generates succinic acid as a commodity chemical product. The phases of research have included strain development and process development. Though we continue to work on one important component of strain development, the research has principally focused on process development. I… more
Date: July 31, 2006
Creator: Eiteman, Mark A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Molecular Mechanisms of Radiation-Induced Genomic Instability in Human Cells

Description: There are many different model systems that have been used to study chromosome instability. What is clear from all these studies is that conclusions concerning chromosome instability depend greatly on the model system and instability endpoint that is studied. The model system for our studies was the human B-lymphoblastoid cell line TK6. TK6 was isolated from a spontaneously immortalized lymphoblast culture. Thus there was no outside genetic manipulation used to immortalize them. TK6 is a relati… more
Date: October 31, 2005
Creator: Liber, Howard L. & Schwartz, Jeffrey L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Characterization of the organic-sulfur-degrading enzymes. Technical report, June 1, 1992--August 31, 1992

Description: Our immediate objective of this project is to characterize and purify the enzymes involved in degrading organic sulfur in coal from two well characterized organic sulfur-degrading strains, IGTS8 and K3B. The purification of these enzymes will facilitate the analysis of the structural organization of the genes encoding these enzymes. The characterization of these enzymes will be able to determine the true value of these enzymes towards the development of a practical process for coal desulfurizat… more
Date: December 31, 1992
Creator: Ho, N. W. Y.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

[The biochemistry, bioenergetics, and physiology of the CO-dependent growth of Rhodospirillum rubrum]. Progress report, March 15, 1990--[1992]

Description: We have previously purified and characterized the holo and Ni-deficient forms of carbon monoxide dehydrogenase (CODH) from Rhodospirillum rubrum, developed protocols for insertion of various metals into the Ni site of the enzyme and characterized these metal substituted forms kinetically. In the current grant period a working hypothesis for the structure of the NiFeS center at the active site of CODH has been developed. A 22 kD FeS protein, which serves as the direct electron acceptor from CODH… more
Date: December 31, 1992
Creator: Ludden, P. W. & Roberts, G. P.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Genetic engineering of sulfur-degrading Sulfolobus. Final technical report, September 1, 1990--August 31, 1991

Description: The objectives of the proposed research is to first establish a plasmid-mediated genetic transformation system for the sulfur degrading Sulfolobus, and then to clone and overexpress the genes encoding the organic-sulfur-degrading enzymes from Sulfolobus- as well as from other microorganisms, to develop a Sulfolobus-based microbial process for the removal of both organic and inorganic sulfur from coal.
Date: December 31, 1991
Creator: Ho, N. W. Y.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Genetics of bacteria that oxidize one-carbon compounds. Progress report, March 1, 1991--June 30, 1993

Description: In the past several years researchers have identified at least 20 genes whose products were required for the oxidation of methanol to formaldehyde in three different facultative methylotrophic bacteria. These genes include structural genes for a cytochrome c{sub L} (mox G) and is a specific electron acceptor for methanol dehydrogenase (MDH), and the two structural genes that encode the large subunit (mox F) and smaller subunit (mox I) of MDH. Other genes are required for the synthesis of the pr… more
Date: December 31, 1993
Creator: Hanson, R. S.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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