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Responses of Cultured Neuronal Networks to the Cannabinoid Mimetic Anandamide

Description: The effects of cannabinoid agonists on spontaneous neuronal network activity were characterized in murine spinal cord and auditory cortical cultures with multichannel extracellular recording using photoetched electrode arrays. Different cultures responded reproducibly with global decreases of spiking and bursting to anandamide and methanandamide, but each agonist showed unique minor effects on network activity. The two tissues responded in a tissue-specific manner. Spontaneous activity in spina… more
Date: May 1998
Creator: Morefield, Samantha I. (Samantha Irene)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Unique applications of cultured neuronal networks in pharmacology, toxicology, and basic neuroscience

Description: This dissertation research explored the capabilities of neuronal networks grown on substrate integrated microelectrode arrays in vitro with emphasis on utilizing such preparations in three specific application domains: pharmacology and drug development, biosensors and neurotoxicology, and the study of burst and synaptic mechanisms. Chapter 1 details the testing of seven novel AChE inhibitors, demonstrating that neuronal networks rapidly detect small molecular differences in closely related comp… more
Date: May 2001
Creator: Keefer, Edward W.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Neuronal Network Analyses in vitro of Acute Individual and Combined Responses to Fluoxetine and Ethanol

Description: Embryonic murine neuronal networks cultured on microelectrode arrays were used to quantify acute electrophysiological effects of fluoxetine and ethanol. Spontaneously active frontal cortex cultures showed highly repeatable, dose-dependent sensitivities to both compounds. Cultures began to respond to fluoxetine at 3 µM and were shut off at 10-16 µM. EC50s mean ± S.D. for spike and burst rates were 4.1 ± 1.5 µM and 4.5 ± 1.1 µM (n=14). The fluoxetine inhibition was reversible and without effect… more
Date: August 2002
Creator: Xia,Yun
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Improved Fabrication and Quality Control of Substrate Integrated Microelectrode Arrays

Description: Spontaneously active monolayer neuronal networks cultured on photoetched multimicroelectrode plates (MMEPs) offer great potential for use in studying neuronal networks. However, there are many problems associated with frequent, long-term use of MMEPs. The major problems include (1) polysiloxane insulation deterioration and breakdown, (2) and loss of gold at the gold electroplated indium-tin oxide (ITO) electrodes. The objective of this investigation was to correct these major problems. Qual… more
Date: May 2000
Creator: Zim, Bret E.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Medial Medulla Networks in Culture: a Multichannel Electrophysiologic and Pharmacological Study

Description: Spontaneously active primary cultures obtained from dissociated embryonic medial medulla tissue were grown on microelectrode arrays for investigating burst patterns and pharmacological responses of respiratory-related neurons. Multichannel burst rates and spike production were used as primary variables for analysis. Pacemaker-like neurons were identified by continued spiking under low Ca++/high Mg++conditions. The number of pacemakers increased with time under synaptic blocking medium. Sensitiv… more
Date: August 1998
Creator: Keefer, Edward W. (Edward Wesley)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Superbursts: Investigation of Abnormal Paroxysmal Bursting Activity in Nerve Cell Networks In Vitro

Description: Superbursts (SBs) are large, seemingly spontaneous activity fluctuations often encountered in high density neural networks in vitro. Little effort has been put forth to define and analyze SBs which are paroxysmal bursting discharges. Through qualitative and quantitative means, I have described specific occurrences of superbursting activity. A complex of paroxysmal bursting has been termed a "superburst episode," and each individual SB is a "superburst event" which is comprises a fine burst stru… more
Date: May 2018
Creator: Suri, Nikita
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Optimization of Cell Culture Procedures for Growing Neural Networks on Microelectrode Arrays

Description: This thesis describes the development of an optimized method for culturing dissociated, monolayer neuronal networks from murine frontal cortex and midbrain. It is presented as a guidebook for use by cell culture specialists and laboratory personnel who require updated and complete procedures for use with microelectrode array (MEA) recording technology. Specific cell culture protocols, contamination prevention and control, as well common problems encountered within the cell culture facility, are… more
Date: December 2007
Creator: Santa Maria, Cara L.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Concentration-dependent Effects of D-Methylphenidate on Frontal Cortex and Spinal Cord Networks in vitro

Description: Spontaneously active frontal cortex and spinal cord networks grown on microelectrode arrays were used to study effects of D-methylphenidate. These central nervous system tissues have relatively low concentrations of dopaminergic and noradrenergic neurons compared to the richly populated loci, yet exhibit similar neurophysiological responses to methylphenidate. The spontaneous spike activity of both tissues was inhibited in a concentration-dependent manner by serial additions of 1-500 µM methylp… more
Date: December 2004
Creator: Miller, Benjamin R.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries

Application of Cultured Neuronal Networks for Use as Biological Sensors in Water Toxicology and Lipid Signaling.

Description: This dissertation research explored the capabilities of neuronal networks grown on substrate integrated microelectrode arrays in vitro to be applied to toxicological research and lipid signaling. Chapter 1 details the effects of chlorine on neuronal network spontaneous electrical activity and pharmacological sensitivity. This study demonstrates that neuronal networks can maintain baseline spontaneous activity, and respond normally to pharmacological manipulations in the present of three times… more
Access: Restricted to the UNT Community Members at a UNT Libraries Location.
Date: August 2004
Creator: Dian, Emese Emöke
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

In Vitro Exploration of Functional Acrolein Toxicity with Cortical Neuronal Networks

Description: Acrolein is produced endogenously after traumatic brain injury (TBI) and is considered a primary mechanism for secondary damage occurring after TBI. We are using frontal cortex networks derived from mouse embryos and grown on microelectrode arrays in vitro to monitor the spontaneous activity of networks and the changes that occur after acrolein application. Networks exposed to acrolein exhibit a biphasic response profile. An initial increase in network activity, followed by a decrease to 100% a… more
Date: May 2018
Creator: Durant, Stormy R.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Investigation of cryopreservation methods for adherent nerve cell networks in vitro.

Description: Cryopreservation in suspension is commonplace for a variety of cell types. However, cryopreservation of adherent cells has achieved limited success. This research aimed to cryopreserve adherent nerve cell networks in vitro in a manner that preserved network morphology and physiology. Successful implementation would enable long term storage of adherent neuronal networks on microelectrode arrays and on-demand access for use in pharmacological and toxicological testing. Based upon morphological as… more
Date: December 2009
Creator: Webb, Veronica Fine
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Acute Effects of the Antibiotic Streptomycin on Neural Network Activity and Pharmacological Responses

Description: The purpose of this study is to find out that if antibiotic streptomycin decreases neuronal network activity or affects the pharmacological responses. The experiments in this study were conducted via MEA (multi-electrode array) technology which records neuronal activity from devices that have multiple small electrodes, serve as neural interfaces connecting neurons to electronic circuitry. The result of this study shows that streptomycin lowered the spike production of neuronal network, and also… more
Date: December 2014
Creator: Zeng, Wei Rong
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Functional and Categorical Analysis of Waveshapes Recorded on Microelectrode Arrays

Description: Dissociated neuronal cell cultures grown on substrate integrated microelectrode arrays (MEAs) generate spontaneous activity that can be recorded for up to several weeks. The signature wave shapes from extracellular recording of neuronal activity display a great variety of shapes with triphasic signals predominating. I characterized extracellular recordings from over 600 neuronal signals. I have preformed a categorical study by dividing wave shapes into two major classes: (type 1) signals in … more
Date: May 2005
Creator: Schwartz, Jacob C.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Evaluation of Zinc Toxicity Using Neuronal Networks on Microelectrode Arrays: Response Quantification and Entry Pathway Analysis

Description: Murine neuronal networks, derived from embryonic frontal cortex (FC) tissue grown on microelectrode arrays, were used to investigate zinc toxicity at concentrations ranging from 20 to 2000 mM total zinc acetate added to the culture medium. Continual multi-channel recording of spontaneous action potential generation allowed a quantitative analysis of the temporal evolution of network spike activity generation at specific zinc acetate concentrations. Cultures responded with immediate concentratio… more
Date: August 2007
Creator: Parviz, Maryam
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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