Aryl Hydrocarbon Hydroxylase and Sixteen Alpha Hydroxylase in Cultured Human Lymphocytes
Description:
Cultured human lymphocytes may be assayed for aryl hydrocarbon hydroxylase (AHH) in whole cell preparations. The optimum assay conditions are pH 8.5, and 1.5 mM Mg++. The reaction is linear with time and cell number, and is inhibited by CO. Estradiol may inhibit induction of AHH by 3-methylcholanthrene, but is a poor competitor for the enzyme. A Caucasian population was assayed for AHH activity. The distribution was lognormal; no difference was found in cultured cells from males and females or …
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Date:
December 1975
Creator:
Coomes, Marguerite L.
Partner:
UNT Libraries