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National Bioenergy Center Biochemical Platform Integration Project Quarterly Update, Fall 2010

Description: Quarterly newsletter describing the activities of the National Bioenergy Center's Biochemical Platform Integration Project including ongoing research and goals. Issue topics: rapid analysis models for compositional analysis of intermediate process streams; engineered arabinose-fermenting Zymomonas mobilis strain.
Date: December 2010
Creator: National Renewable Energy Laboratory (U.S.)
Partner: UNT Libraries Government Documents Department
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National Bioenergy Center Biochemical Platform Integration Project Quarterly Update, Number 28, Spring 2011

Description: Quarterly newsletter describing the activities of the National Bioenergy Center's Biochemical Platform Integration Project including ongoing research and goals. Issue topics: 33rd Symposium on Biotechnology for Fuels and Chemicals program sessions and special topic sessions; assessment of waste water treatment needs; and an update on new arabinose-to-ethanol fermenting Zymomonas mobilis strains.
Date: April 2011
Creator: National Renewable Energy Laboratory (U.S.)
Partner: UNT Libraries Government Documents Department
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Ethanologenic Enzymes of Zymomonas mobilis

Description: Zymomonas mobilis is a unique microorganism in being both obligately fermentative and utilizing a Entner-Doudoroff pathway for glycolysis. Glycolytic flux in this organism is readily measured as evolved carbon dioxide, ethanol, or glucose consumed and exceeds 1 {micro}mole glucose/min per mg cell protein. To support this rapid glycolysis, approximately 50% of cytoplasmic protein is devoted to the 13 glycolytic and fermentative enzymes which constitute this central catabolic pathway. Only 1 ATP … more
Date: March 1, 1999
Creator: Ingram, Lonnie O'Neal
Partner: UNT Libraries Government Documents Department
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Control of sugar transport and metabolism in Zymomonas mobilis. Final report

Description: This research deals with the physiology and genetics of sugar transport and metabolic control in Zymomonas mobilis. The specific objectives of the grant as as follows: First, the complex interactions of transcriptional, post-transcriptional and translational control mechanisms on regulation of the glf operon will be investigated. Second, the structure and function of the unique glucose facilitator will be examined by a combination of in vitro and in vivo approaches, making use of the geneticall… more
Date: September 1, 1995
Creator: Conway, T.
Partner: UNT Libraries Government Documents Department
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Ethanol Production from Glucose and Xylose by Immobilized <i>Zymomonas mobilis</i> CP4(pZB5)

Description: Fermentation of glucose-xylose mixtures to ethanol was investigated in batch and continuous experiments using immobilized recombinant <i>Zymomonas mobilis</i> CP4(pZB5). This microorganism was immobilized by entrapment in k-carrageenan beads having a diameter of 1.5-2.5 mm. Batch experiments showed that the immobilized cells co-fermented glucose and xylose to ethanol and that the presence of glucose improved the xylose utilization rate. Batch fermentation of rice straw hydrolyzate containing 76… more
Date: May 2, 1999
Creator: Blanco, M.; Davison, B. H.; Krishnan, M. S.; Nghiem, N. P. & Shattuck, C. K.
Partner: UNT Libraries Government Documents Department
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Engineered Microbial Consortium for the Efficient Conversion of Biomass to Biofuels

Description: Current energy and environmental challenges are driving the use of cellulosic materials for biofuel production. A major obstacle in this pursuit is poor ethanol tolerance among cellulolytic Clostridium species. The first objective of this work was to establish a potential upper boundary of ethanol tolerance for the cellulosome itself. The hydrolytic function of crude cellulosome extracts from C. cellulolyticum on carboxymethyl cellulose (CMC) with 0, 5, 10, 15, 20 and 25% (v/v) ethanol was dete… more
Date: August 2014
Creator: Anieto, Ugochukwu Obiakornobi
Partner: UNT Libraries
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Ethanol production from dry-mill corn starch in a fluidized-bed bioreactor

Description: The development of a high-rate process for the production of fuel ethanol from dry-mill corn starch using fluidized-bed bioreactor (FBR) technology is discussed. Experiments were conducted in a laboratory scale FBR using immobilized biocatalysts. Two ethanol production process designs were considered in this study. In the first design, simultaneous saccharification and fermentation was performed at 35 C using {kappa}-carageenan beads (1.5 mm to 1.5 mm in diameter) of co-immobilized glucoamylase… more
Date: August 1, 1998
Creator: Krishnan, M. S.; Nghiem, N. P. & Davison, B. H.
Partner: UNT Libraries Government Documents Department
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Continuous Ethanol Production Using Immobilized-Cell/Enzyme Biocatalysts in Fluidized-Bed Bioreactor (FBR)

Description: The immobilized-cell fluidized-bed bioreactor (FBR) was developed at Oak Ridge National Laboratory (ORNL). Previous studies at ORNL using immobilized Zymomonas mobilis in FBR at both laboratory and demonstration scale (4-in-ID by 20-ft-tall) have shown that the system was more than 50 times as productive as industrial benchmarks (batch and fed-batch free cell fermentations for ethanol production from glucose). Economic analysis showed that a continuous process employing the FBR technology to pr… more
Date: November 16, 2003
Creator: Nghiem, NP
Partner: UNT Libraries Government Documents Department
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Conversion of bagasse cellulose into ethanol

Description: The study conducted by Arkenol was designed to test the conversion of feedstocks such as sugar cane bagasse, sorghum, napier grass and rice straw into fermentable sugars, and then ferment these sugars using natural yeasts and genetically engineered Zymomonis mobilis bacteria (ZM). The study did convert various cellulosic feedstocks into fermentable sugars utilizing the patented Arkenol Concentrated Acid Hydrolysis Process and equipment at the Arkenol Technology Center in Orange, California. The… more
Date: November 19, 1997
Creator: Cuzens, J.E.
Partner: UNT Libraries Government Documents Department
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Production of ethanol from starch by co-immobilized Zymomonas mobilis -- Glucoamylase in a fluidized-bed reactor

Description: The production of ethanol from starch was studied in a fluidized-bed reactor (FBR) using co-immobilized Zymomonas mobilis and glucoamylase. The FBR was a glass column of 2.54 cm in diameter and 120 cm in length. The Z. mobilis and glucoamylase were co-immobilized within small uniform beads (1.2 to 2.5 mm diameter) of {kappa}-carrageenan. The substrate for ethanol production was a soluble starch. Light steep water was used as the complex nutrient source. The experiments were performed at 35 C an… more
Date: August 1, 1997
Creator: Sun, M. Y.; Davison, B. H.; Bienkowski, P. R.; Nghiem, N. P. & Webb, O.
Partner: UNT Libraries Government Documents Department
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Final Technical Report: Improvement of Zymomonas mobilis for Commercial Use in Corn-based Biorefineries

Description: Between 2007 and 2010 DuPont conducted a program under DOE award DE-FC36-07GO17056 to develop and improve Zymomonas mobilis as an ethanologen for commercial use in biorefineries to produce cellulosic ethanol. This program followed upon an earlier DOE funded program in which DuPont, in collaboration with the National Renewable Energy Laboratory (NREL) had developed a Zymomonas strain in conjunction with the development of an integrated cellulosic ethanol process. In the current project, we sough… more
Date: December 7, 2010
Creator: Hitz, William D.
Partner: UNT Libraries Government Documents Department
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Ethanol Production from Rice-Straw Hydrolysate Using Zymomonas Mobilis in a Continuous Fluidized-Bed Reactor (FBR)

Description: Rice-straw hydrolysate obtained by the Arkenol's concentrated acid hydrolysis process was fermented to ethanol using a recombinant Zymomonas mobilis strain capable of utilizing both glucose and xylose in a continuous fluidized-bed reactor (FBR). The parameters studied included biocatalyst stability with and without antibiotic, feed composition, and retention time. Xylose utilization in the presence of tetracycline remained stable for at least 17 days. This was a significant improvement over the… more
Date: January 1, 2001
Creator: deJesus, D. & Nghiem, N. P.
Partner: UNT Libraries Government Documents Department
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Modeling scaleup effects on a small pilot-scale fluidized-bed reactor for fuel ethanol production

Description: Domestic ethanol use and production are presently undergoing significant increases along with planning and construction of new production facilities. Significant efforts are ongoing to reduce ethanol production costs by investigating new inexpensive feedstocks (woody biomass) and by reducing capital and energy costs through process improvements. A key element in the development of advanced bioreactor systems capable of very high conversion rates is the retention of high biocatalyst concentratio… more
Date: September 1995
Creator: Webb, O. F.; Davison, B. H. & Scott, T. C.
Partner: UNT Libraries Government Documents Department
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Overcoming the Recalcitrance of Cellulosic Biomass by Value Prior to Pulping: Cooperative Research and Development Final Report, CRADA Number CRD-07-221

Description: The Value Prior to Pulping (VPP) project goal was to demonstrate the technical and commercial feasibility of introducing a new value stream into existing pulp and paper mills. Essentially the intent was to transfer the energy content of extracted hemicellulose from electricity and steam generated in the recovery boiler to a liquid transportation fuel. The hemicellulose fraction was extracted prior to pulping, fractionated, or conditioned if necessary, and fermented to ethanol. Commercial adapta… more
Date: April 1, 2012
Creator: Lowell, A.
Partner: UNT Libraries Government Documents Department
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Three immobilized-cell columnar bioreactors for enhanced production of commodity chemicals

Description: Immobilized-cell fluidized-bed bioreactors (FBRS) can be used with a variety of fermentations to increase production of fuels, solvents, organic acids, and other fermentation products. Part of the increased rates and yields are due to the immobilization of the biocatalyst at high concentrations. This FBR system with immobilized Zymomonas mobiles increased ethanol productivity more than tenfold with 99% conversion and near stoichiometric yields. FBRs also offer several additional modes of operat… more
Date: July 1, 1993
Creator: Davison, B. H.; Scott, C. D. & Kaufman, E. N.
Partner: UNT Libraries Government Documents Department
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Novel Biomass Conversion Process Results in Commercial Joint Venture; The Spectrum of Clean Energy Innovation (Fact Sheet)

Description: Fact sheet describing DuPont/NREL cooperative research and development agreement that resulted in biomass-to-ethanol conversion process used as a basis for DuPont Danisco Cellulosic Ethanol, LLC and cellulosic ethanol demonstration plant.
Date: June 1, 2010
Partner: UNT Libraries Government Documents Department
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Continuous production of ethanol by use of flocculent Zymomonas mobilis

Description: Improved means and process for producing ethanol by fermentation are provided. Another object of the invention is to produce ethanol in a continuous-flow process by means of a biological catalyst that can be retained in a continuous-flow reactor vessel without being bonded to or held within a support material. An additional object of the invention is to provide a fermentation reactor vessel wherein disturbance of the desirable plug flow of sugar solution is minimized. These objects are attained… more
Date: January 28, 1982
Creator: Arcuri, E. J. & Donaldson, T. L.
Partner: UNT Libraries Government Documents Department
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