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Plant cell wall architecture. Final technical report for DOE award no. DE-FG02-97ER20258

Description: The goals of the project were to investigate the roles of caffeoyl coenzyme A O-methyltransferase (CCoAOMT), an enzyme involved in the phenylpropanoid biosynthetic pathway, in the biosynthesis of lignin. The investigators proposed to analyze the expression pattern of CCoAOMT in plants, and examine how reduction in the expression of CCoAOMT would affect lignin content and composition. The goals were fulfilled, and significant findings on lignin biochemistry were made. Two papers were published, … more
Date: August 6, 2002
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Site Directed Mutagenesis of β-Ketoadipate Succinyl-Coenzyme A Transferase II from Acinetobacter Calcoaceticus

Description: The role of specific amino acid residues in β-ketoadipate succinyl-coenzyme A transferase II from Acinetobacter calcoaceticus was investigated. A 1412 base pair BamiHI-EcoRI fragment carrying the catIJ genes was amplified by polymerase chain reaction and inserted into pUCl9 to generate the plasmid pCATEl9. Escherichia coli DH5α (pCATEl9) carrying only the catlJ genes expressed 3-fold higher enzyme activity than the parent strain. Two mutants were constructed by site directed mutagenesis so that… more
Date: August 1993
Creator: Sheng, Mei
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Cancer prevention: Application of chemicals in treatment therapy to reduce risk in secondary tumor induction

Description: We have investigated WR2721 and similar aminothiol compounds to determine their role in modulating the mutagenic and carcinogenic action of radiation and selected chemotherapeutic agents. We have found that 2-((aminopropyl)amino) ethanethiol (WR1065), a free-thiol of WR2721, effectively protected against the mutagenic effects of radiation at the HGPRT locus in V79 cells even if administered up to 3 h following irradiation, a reduction in mutation frequencies of about 0.55. Under the conditions … more
Date: January 1, 1986
Creator: Grdina, D. J.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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[An homologous recombination strategy to directly clone mammalian telemeres]

Description: We have pursued three goals over the past year. The first involved determining whether the HARY vector could be used for homologous integration in the human genome. The second was to ascertain whether inserted sequences could be amplified in preference to the endogenous DHFR genes. The third was to determine if the HARY insertion could provide an anchor point for long range restriction mapping. The progress in each goal is described.
Date: January 1, 1992
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Tissue Enzyme Changes in Parabiotic Rats with Subcutaneous Lymphoma

Description: A solid lymphoma was implanted into normal rats and into one partner of parabiotic pairs. Appreciable decreases in hepatic ornithine aminotransferase and arginase occurred about a week earlier (4 to 6 days after implantation) in single than in parabiotic hosts. By 18 to 21 days significant decreases in both enzymes were also apparent in the host partner. The hepatic thymidine kinase showed a 5-fold elevation in single hosts 4 days after implantation; by 14 days its levels were about 200 times a… more
Date: January 1, 1977
Creator: Herzfeld, Annemarie; Greengard, Olga & Warren, Shields
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Mapping strategies: Chromosome 16 workshop

Description: The following topics from a workshop on chromosome 16 are briefly discussed: genetic map of chromosome 16; chromosome breakpoint map of chromosome 16; integrated physical/genetic map of chromosome 16; pulsed field map of the 16p13.2--p13.3 region (3 sheets); and a report of the HGM10 chromosome 16 committee.
Date: January 1, 1989
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

(Physiology and genetics of metabolic flux control in Zymomonas mobilis)

Description: The funded research deals with the physiology and genetics of glycolytic flux control in Zymomonas mobilis. Two fundamental biological questions are begin addressed: First, how do the enzymes of glycolytic pathways act in concert to regulate metabolic flux Second, what is the role of gene expression in regulating high level synthesis of the glycolytic enzymes in a balance that allows proper glycolytic flux control The specific objectives of the grant are as follows: 1. To clone the structural a… more
Date: January 1, 1992
Creator: Conway, T.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Regulation of cell division in higher plants

Description: Cell division is arguably the most fundamental of all developmental processes. In higher plants, mitotic activity is largely confined to foci of patterned cell divisions called meristems. From these perpetually embryonic tissues arise the plant's essential organs of light capture, support, protection and reproduction. Once an adequate understanding of plant cell mitotic regulation is attained, unprecedented opportunities will ensue for analyzing and genetically controlling diverse aspects of de… more
Date: January 1, 1992
Creator: Jacobs, T.W.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Cellular energy metabolism

Description: Studies have been carried out on adenylate kinase which is an important enzyme in determining the concentrations of the adenine nucleotides. An efficient method has been developed to clone mutant adenylate kinase genes in E. coli. Site-specific mutagenesis of the wild type gene also has been used to obtain forms of adenylate kinase with altered amino acids. The wild type and mutant forms of adenylate kinase have been overexpressed and large quantities were readily isolated. The kinetic and fluo… more
Date: June 1, 1991
Creator: Glaser, M.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Monochromosomal hybrids for the analysis of the human genome

Description: In this research project the authors proposed to develop rodent/human hybrid cell lines each containing a single different human chromosome. The human chromosomes will be marked with Ecogpt and stably maintained by selection in the hybrid cells. The experimental approach to produce the proposed cell lines involve the following: they will first transfer a cloned selectable marker, Ecogpt (an E. coli gene for xanthine-guanine phosphoribosyltransferase: XGPRT) to normal diploid human cells using a… more
Date: January 1, 1990
Creator: Athwal, R. S.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Enzymatic studies of radiation damage. Progress report, July 1, 1976-September 30, 1977

Description: The major accomplishments of the year were a new method and a totally unexpected discovery that venom phosphodiesterase belongs to the group of single-strand specific nucleases. It acts on viral duplex DNA by producing a series of shorter fragments of duplex DNA. It was also found that the relaxed circular DNA of PM2 virus, that was previously enzymatically ligated, may be opened by very high amounts of phosphodiesterase. The new method deals with a simplified way of preparing polynucleotide ki… more
Date: June 1, 1977
Creator: Laskowski, M., Sr.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Laminin Mediates Tissue-specific Gene Expression in Mammary Epithelia

Description: Tissue-specific gene expression in mammary epithelium is dependent on the extracellular matrix as well as hormones. There is good evidence that the basement membrane provides signals for regulating beta-casein expression, and that integrins are involved in this process. Here, we demonstrate that in the presence of lactogenic hormones, laminin can direct expression of the beta-casein gene. Mouse mammary epithelial cells plated on gels of native laminin or laminin-entactin undergo functional diff… more
Date: April 1, 1995
Creator: Streuli, Charles H; Schmidhauser, Christian; Bailey, Nina; Yurchenco, Peter; Skubitz, Amy P. N.; Roskelley, Calvin et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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[Characterization and modification of phage T7 DNA polymerase for use in DNA sequencing]: Progress report

Description: This project focuses on the DNA polymerase and accessory proteins of phage T7 for use in DNA sequence analysis. T7 DNA polymerase (gene 5 protein) interacts with accessory proteins for the acquisition of properties such as processivity that are necessary for DNA replication. One goal is to understand these interactions in order to modify the proteins to increase their usefulness with DNA sequence analysis. Using a genetically modified gene 5 protein lacking 3' to 5' exonuclease activity we have… more
Date: January 1, 1992
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Involvement of recombination in x-ray mutagenesis of human cells

Description: Closely related human lymphoblastoid cell lines derived from WI-L2 differ greatly in their responses to X-irradiation. Compared with TK6 (ATCC CRL 8015), WI-L2-NS (ATCC CRL 8155) has an enhanced X-ray survival. The induction of mutation by X-rays is also markedly different. The hemizygous hprt locus is slightly more mutable in WI-L2-NS than in TK6, and the dose response fits best to a linear-quadratic curve rather than the linear fit of TK6X-ray induced mutation at the autosomal tk locus in het… more
Date: January 1, 1993
Creator: Amundson, S. A. (Los Alamos National Lab., NM (United States)); Xia, F. & Liber, H. L. (Harvard School of Public Health, Boston, MA (United States))
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol

Description: BackgroundIncreasing energy costs and environmental concerns have motivated engineering microbes for the production of ?second generation? biofuels that have better properties than ethanol.Results& ConclusionsSaccharomyces cerevisiae was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms (S. cerevisiae, Escherichia coli, Clostridium beijerinckii, and Ralstonia eutropha) were substituted for the Clostridial enzymes and their effect on n-… more
Date: November 25, 2008
Creator: Steen, EricJ.; Chan, Rossana; Prasad, Nilu; Myers, Samuel; Petzold, Christopher; Redding, Alyssa et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Viruses of eukaryotic green algae; Progress report, June 20, 1990--July 1, 1991

Description: Many large polyhedral, dsDNA containing (ca. 330 kb), plaque forming viruses which infect a unicellular, eukaryotic, chlorella-like green alga have been isolated and characterized. The plaque assay, the ability to synchronously infect the host, the short life cycle, and the ability of the viruses to undergo homologous recombination make them excellent model systems for studying many plant cell functions in the manner that bacterial and animal viruses have been used to study bacterial and animal… more
Date: December 31, 1991
Creator: Van Etten, J.L.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Inducible error-prone repair in B. subtilis. Final report, September 1, 1979-June 30, 1981

Description: The research performed under this contract has been concentrated on the relationship between inducible DNA repair systems, mutagenesis and the competent state in the gram positive bacterium Bacillus subtilis. The following results have been obtained from this research: (1) competent Bacillus subtilis cells have been developed into a sensitive tester system for carcinogens; (2) competent B. subtilis cells have an efficient excision-repair system, however, this system will not function on bacteri… more
Date: June 1, 1981
Creator: Yasbin, R. E.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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Biochemical and Molecular Analysis of a Transmembrane Protein Kinase From Arabidopsis Thaliana

Description: We have isolated genomic and cDNA clones encoding a novel receptor-like protein kinase from the higher plant Arabidopsis thaliana. This kinase is being studied by combining biochemical, molecular, and genetic approaches. Domain-specific antibodies immunodecorate a polypeptide with a molecular mass of 120,000 daltons in extracts of Arabidopsis, where it has been found in all portions of the plant examined including root, stem, leaf, flower, and silique. Cytochemical analysis and initial studies … more
Date: January 1, 1993
Creator: Bleecker, A. B.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Thioredoxin binding site of phosphoribulokinase overlaps the catalytic site. [R]

Description: The ATP-regulatory binding site of phosphoribulokinase was studied using bromoacetylethanolamine phosphate (BrAcNHEtOP). BrAcNHEtOP binds to the active-regulatory binding site of the protein. Following trypsin degradation of the labeled protein, fragments were separated by HPLC and sequenced. (DT)
Date: January 1, 1986
Creator: Porter, M. A. & Hartman, F. C.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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