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Electroencephalographic Events During the Wisconsin Card Sorting Test

Description: Quantitative electroencephalography (QEEG) was used in this study to describe cognitive processing, particularly brain locations used, during performance of the Wisconsin Card Sorting Test (WCST). The hypothesis was that significant cognitive functioning is not limited to the frontal lobes. Significant EEG activity was found in non-frontal areas as well as frontal areas.
Date: August 1998
Creator: DeBeus, Mary
Partner: UNT Libraries

Self-organization of engineered epithelial tubules by differential cellular motility

Description: Patterning of developing tissues arises from a number of mechanisms, including cell shape change, cell proliferation, and cell sorting from differential cohesion or tension. Here, we reveal that differences in cell motility can also lead to cell sorting within tissues. Using mosaic engineered mammary epithelial tubules, we found that cells sorted depending on their expression level of the membrane-anchored collagenase matrix metalloproteinase (MMP)-14. These rearrangements were independent of the catalytic activity of MMP14 but absolutely required the hemopexin domain. We describe a signaling cascade downstream of MMP14 through Rho kinase that allows cells to sort within the model tissues. Cell speed and persistence time were enhanced by MMP14 expression, but only the latter motility parameter was required for sorting. These results indicate that differential directional persistence can give rise to patterns within model developing tissues.
Date: February 4, 2009
Creator: Mori, Hidetoshi; Gjorevski, Nikolce; Inman, Jamie L; Bissell, Mina J & Nelson, Celeste M
Partner: UNT Libraries Government Documents Department

Widespread Discordance of Gene Trees with Species Tree inDrosophila: Evidence for Incomplete Lineage Sorting

Description: The phylogenetic relationship of the now fully sequencedspecies Drosophila erecta and D. yakuba with respect to the D.melanogaster species complex has been a subject of controversy. All threepossible groupings of the species have been reported in the past, thoughrecent multi-gene studies suggest that D. erecta and D. yakuba are sisterspecies. Using the whole genomes of each of these species as well as thefour other fully sequenced species in the subgenus Sophophora, we set outto investigate the placement of D. erecta and D. yakuba in the D.melanogaster species group and to understand the cause of the pastincongruence. Though we find that the phylogeny grouping D. erecta and D.yakuba together is the best supported, we also find widespreadincongruence in nucleotide and amino acid substitutions, insertions anddeletions, and gene trees. The time inferred to span the two keyspeciation events is short enough that under the coalescent model, theincongruence could be the result of incomplete lineage sorting.Consistent with the lineage-sorting hypothesis, substitutions supportingthe same tree were spatially clustered. Support for the different treeswas found to be linked to recombination such that adjacent genes supportthe same tree most often in regions of low recombination andsubstitutions supporting the same tree are most enriched roughly on thesame scale as linkage disequilibrium, also consistent with lineagesorting. The incongruence was found to be statistically significant androbust to model and species choice. No systematic biases were found. Weconclude that phylogenetic incongruence in the D. melanogaster speciescomplex is the result, at least in part, of incomplete lineage sorting.Incomplete lineage sorting will likely cause phylogenetic incongruence inmany comparative genomics datasets. Methods to infer the correct speciestree, the history of every base in the genome, and comparative methodsthat control for and/or utilize this information will be valuableadvancements for the field of comparative genomics.
Date: August 28, 2006
Creator: Pollard, Daniel A.; Iyer, Venky N.; Moses, Alan M. & Eisen,Michael B.
Partner: UNT Libraries Government Documents Department

The Sort on Radioactive Waste Type Model: A method to sort single-shell tanks into characteristics groups

Description: The Sort on Radioactive Waste Type (SORWT) Model is a method to categorize Hanford Site single-shell tanks (SSTS) into groups of tanks expected to exhibit similar chemical and physical characteristics based on their major waste types and processing histories. The model has identified 24 different waste-type groups encompassing 133 of the 149 SSTs and 93% of the total waste volume in SSTS. The remaining 16 SSTs and associated wastes could not be grouped. according to the established criteria and were placed in an ungrouped category. A detailed statistical verification study has been conducted that employs analysis of variance (ANOVA) and the core sample analysis data collected since 1989. These data cover eight tanks and five SORWT groups. The verification study showed that these five SORWT groups are highly statistically significant; they represent approximately 10% of the total waste volume and 26% of the total sludge volume in SSTS. Future sampling recommendations based on the SORWT Model results include 32 core samples from 16 tanks and 18 auger samples from six tanks. Combining these data with the existing body of information will form the basis for characterizing 98 SSTs (66%). These 98 SSTs represent 78% of the total waste volume, 61% of the total sludge volume, and 88 % of the salt cake volume.
Date: February 1, 1995
Creator: Hill, J.G.; Anderson, G.S. & Simpson, B.C.
Partner: UNT Libraries Government Documents Department

Fuel sorting evaluation

Description: An evaluation of functions and requirements associated with sorting fuel from the Hanford K Basins is presented to support design issue resolution decisions for achieving interim fuel storage. Potential requirements are recommended for implementation in design activities. The recommendations are provided as input to a management decision process where decisions are finalized and the sorting issue is closed.
Date: March 12, 1996
Creator: Pajunen, A.L.
Partner: UNT Libraries Government Documents Department

Hash sorter - firmware implementation and an application for the Fermilab BTeV level 1 trigger system

Description: A hardware hash sorter for the Fermilab BTeV Level 1 trigger system will be presented. The has sorter examines track-segment data before the data are sent to a system comprised of 2500 Level 1 processors, and rearranges the data into bins based on the slope of track segments. They have found that by using the rearranged data, processing time is significantly reduced allowing the total number of processors required for the Level 1 trigger system to be reduced. The hash sorter can be implemented in an FPGA that is already included as part of the design of the trigger system. Hash sorting has potential applications in a broad area in trigger and DAQ systems. It is a simple O(n) process and is suitable for FPGA implementation. Several implementation strategies will also be discussed in this document.
Date: November 5, 2003
Creator: al., Jinyuan Wu et
Partner: UNT Libraries Government Documents Department

Sorting chromatic sextupoles for easily and effectively correcting second order chromaticity in the Relativistic Heavy Ion Collider

Description: Based on the contributions of the chromatic sextupole families to the half-integer resonance driving terms, we discuss how to sort the chromatic sextupoles in the arcs of the Relativistic Heavy Ion Collider (RHIC) to easily and effectively correct the second order chromaticities. We propose a method with 4 knobs corresponding to 4 pairs of chromatic sextupole families to online correct the second order chromaticities. Numerical simulation justifies this method, showing that this method reduces the unbalance in the correction strengths of sextupole families and avoids the reversal of sextupole polarities. Therefore, this method yields larger dynamic apertures for the proposed RHIC 2009 100GeV polarized proton run lattices.
Date: January 2, 2009
Creator: Luo,Y.; Tepikian, S.; Fischer, W.; Robert-Demolaize, G. & Trbojevic, D.
Partner: UNT Libraries Government Documents Department

Hereditary Spherocytosis and Hereditary Elliptocytosis: Aberrant Protein Sorting during Erythroblast Enucleation

Description: During erythroblast enucleation, membrane proteins distribute between extruded nuclei and reticulocytes. In hereditary spherocytosis (HS) and hereditary elliptocytosis (HE), deficiencies of membrane proteins, in addition to those encoded by the mutant gene, occur. Elliptocytes, resulting from protein 4.1R gene mutations, lack not only 4.1R but also glycophorin C, which links the cytoskeleton and bilayer. In HS resulting from ankyrin-1 mutations, band 3, Rh-associated antigen, and glycophorin A are deficient. The current study was undertaken to explore whether aberrant protein sorting, during enucleation, creates these membrane-spanning protein deficiencies. We found that although glycophorin C sorts to reticulocytes normally, it distributes to nuclei in 4.1R-deficient HE cells. Further, glycophorin A and Rh-associated antigen, which normally partition predominantly to reticulocytes, distribute to both nuclei and reticulocytes in an ankyrin-1-deficient murine model of HS. We conclude that aberrant protein sorting is one mechanistic basis for protein deficiencies in HE and HS.
Date: February 8, 2010
Creator: Salomao, Marcela; Chen, Ke; Villalobos, Jonathan; Mohandas, Narla; An, Xiuli & Chasis, Joel Anne
Partner: UNT Libraries Government Documents Department

Soft inertial microfluidics for high throughput separation of bacteria from human blood cells

Description: We developed a new approach to separate bacteria from human blood cells based on soft inertial force induced migration with flow defined curved and focused sample flow inside a microfluidic device. This approach relies on a combination of an asymmetrical sheath flow and proper channel geometry to generate a soft inertial force on the sample fluid in the curved and focused sample flow segment to deflect larger particles away while the smaller ones are kept on or near the original flow streamline. The curved and focused sample flow and inertial effect were visualized and verified using a fluorescent dye primed in the device. First the particle behavior was studied in detail using 9.9 and 1.0 {micro}m particles with a polymer-based prototype. The prototype device is compact with an active size of 3 mm{sup 2}. The soft inertial effect and deflection distance were proportional to the fluid Reynolds number (Re) and particle Reynolds number (Re{sub p}), respectively. We successfully demonstrated separation of bacteria (Escherichia coli) from human red blood cells at high cell concentrations (above 10{sup 8}/mL), using a sample flow rate of up to 18 {micro}L/min. This resulted in at least a 300-fold enrichment of bacteria at a wide range of flow rates with a controlled flow spreading. The separated cells were proven to be viable. Proteins from fractions before and after cell separation were analyzed by gel electrophoresis and staining to verify the removal of red blood cell proteins from the bacterial cell fraction. This novel microfluidic process is robust, reproducible, simple to perform, and has a high throughput compared to other cell sorting systems. Microfluidic systems based on these principles could easily be manufactured for clinical laboratory and biomedical applications.
Date: January 5, 2009
Creator: Wu, Zhigang; Willing, Ben; Bjerketorp, Joakim; Jansson, Janet K. & Hjort, Klas
Partner: UNT Libraries Government Documents Department

AMESH A mesh creating program for the integral finite differencemethod: A User's Manual

Description: Amesh program generates discrete grids for numerical modeling of flow and transport problems in which the formulation is based on integral finite difference method (IFDM). For example, the output of Amesh can be used directly as (part of) the input to TOUGH2 or TOUGH numerical Simulator (Pruess, 1987, 1990, Pruess, et al., 1996). The code Amesh can generate 1D, 2D or 3D numerical grids for a given set of locations, i.e. the centers of each discrete sub-domain. In the 2D aerial plane the Voronoi tessellation method is used (Voronoi, 1908; Ahuja, 1982; Aurehammer, 1991; Fortune, 1987, 1988, 1993). In this method we can create a mesh of elements, within model domain, where the interfaces between neighbor elements are the perpendicular bisectors of the line connecting the element centers. The interface distances are simply the medians of the line connecting the centers. To create the 3D grid, the vertical direction interface areas are always treated as horizontal projections of the 2D areal plane. In the lateral direction the interface areas are always vertical projections. In both cases the direction of gravity vector is given by the cosine of angle formed by the line joining the element centers and the vertical. From the list of element locations (center points), the program determines element volumes, and the connection information, i.e. areas, connection distances and the angle. The default input file is ''in''. The output files are ''eleme'' are ''conne'' and ''segmt''. The files ''eleme'' and ''come'' contain all the data required to describe a TOUGH2 input and together they describe the input TOUGH2 input file called ''MESH'', for the specified domain. The file ''segmt'' can be used to plot the geometrical shape of each element in each layer of the input domain. The input data into Amesh does not have to be ordered. ...
Date: August 31, 1998
Creator: Haukwa, Charles
Partner: UNT Libraries Government Documents Department

Genetic Control of the Trigger for the G2/M Checkpoint

Description: The work undertaken in this project addressed two seminal areas of low dose radiation biology that are poorly understood and controversial. These areas are the challenge to the linear-no-threshold (LNT) paradigm at low doses of radiation and, the fundamental elements of radiation bystander effect biology Genetic contributions to low dose checkpoint engagement: The LNT paradigm is an extrapolation of known, measured cancer induction endpoints. Importantly, data for lower doses is often not available. Debatably, radiation protection standards have been introduced which are prudently contingent on the adherence of cancer risk to the established trend seen at higher doses. Intriguing findings from other labs have hinted at separate DNA damage response programs that engage at low or high levels of radiation. Individual radiation sensitivity commensurate with hemizygosity for a radiation sensitivity gene has been estimated at 1-2% in the U.S.. Careful interrogation of the DNA damage response at low doses of radiation became important and served as the basis for this grant. Several genes were tested in combinations to determine if combined haploinsufficiency for multiple radiosensitizing genes could render a cell more sensitive to lower levels of acute radiation exposure. We measured a classical radiation response endpoint, cell cycle arrest prior to mitosis. Mouse embryo fibroblasts were used and provided a uniform, rapidly dividing and genetically manipulable population of study. Our system did not report checkpoint engagement at acute doses of gamma rays below 100 mGy. The system did report checkpoint engagement reproducibly at 500 mGy establishing a threshold for activation between 100 and 500 mGy. Engagement of the checkpoint was ablated in cells nullizygous for ATM but was otherwise unperturbed in cells combinatorially haploinsufficient for ATM and Rad9, ATM and PTEN or PTEN and Rad9. Taken together, these experiments tell us that, in a sensitive fibroblast culture system, the engagement ...
Date: October 1, 2013
Creator: Hall, Eric J.; Smilenov, Lubomir B. & Young, Erik F.
Partner: UNT Libraries Government Documents Department

Tight bounds on the size of neural networks for classification problems

Description: This paper relies on the entropy of a data-set (i.e., number-of-bits) to prove tight bounds on the size of neural networks solving a classification problem. First, based on a sequence of geometrical steps, the authors constructively compute an upper bound of O(mn) on the number-of-bits for a given data-set - here m is the number of examples and n is the number of dimensions (i.e., R{sup n}). This result is used further in a nonconstructive way to bound the size of neural networks which correctly classify that data-set.
Date: June 1, 1997
Creator: Beiu, V. & Pauw, T. de
Partner: UNT Libraries Government Documents Department

NMT-7 plan for producing certifiable TRU debris waste for WIPP

Description: Analysis of waste characterization data for debris items generated during a recent six month period indicates that the certifiability of TRUPACT II payload containers packaged at the Los Alamos National Laboratory Plutonium Facility (TA-55) can be increased from approximately 52% of solid waste payload containers to 78% by applying the simple strategies of screening out high decay heat items and sorting remaining items to maintain nuclear material loading at levels below WIPP waste acceptance limits. Implementation of these strategies will have negative impacts on waste minimization and waste management operations that must also be considered.
Date: December 1, 1997
Creator: Montoya, A.J.
Partner: UNT Libraries Government Documents Department

An effective waste management process for segregation and disposal of legacy mixed waste at Sandia National Laboratories/New Mexico

Description: Sandia National Laboratories/New Mexico (SNL/NM) is a research and development facility that generates many highly diverse, low-volume mixed waste streams. Under the Federal Facility Compliance Act, SNL/NM must treat its mixed waste in storage to meet the Land Disposal Restrictions treatment standards. Since 1989, approximately 70 cubic meters (2,500 cubic feet) of heterogeneous, poorly characterized and inventoried mixed waste was placed in storage that could not be treated as specified in the SNL/NM Site Treatment Plan. A process was created to sort the legacy waste into sixteen well-defined, properly characterized, and accurately inventoried mixed waste streams (Treatability Groups) and two low-level waste streams ready for treatment or disposal. From June 1995 through September 1996, the entire volume of this stored mixed waste was sorted and inventoried. This process was planned to meet the technical requirements of the sorting operation and to identify and address the hazards this operation presented. The operations were routinely adapted to safely and efficiently handle a variety of waste matrices, hazards, and radiological conditions. This flexibility was accomplished through administrative and physical controls integrated into the sorting operations. Many Department of Energy facilities are currently facing the prospect of sorting, characterizing, and treating a large inventory of mixed waste. The process described in this report is a proven method for preparing a diverse, heterogeneous mixed waste volume into segregated, characterized, inventoried, and documented waste streams ready for treatment or disposal.
Date: April 1, 1998
Creator: Hallman, A.K.; Meyer, D.; Rellergert, C.A. & Schriner, J.A.
Partner: UNT Libraries Government Documents Department

Molecular characterization of flow-sorted mammalian centromeres

Description: This is the final report of a three-year, Laboratory Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). The project involved experiments directed towards developing a molecular characterization of the centromere region of mammalian chromosomes. Attempts to purify this essential chromosomal locus by conventional methods have thus far been unsuccessful. However, preliminary data obtained in collaboration with the National Flow Cytometry Resource (NFCR) showed that it is possible to purify a chromosome fragment that is present in certain cultured mouse cell lines and has all the properties expected of an intact centromere region. To begin sorting this minichromosome for the identification of proteins preferentially associated with centromere regions, standard buffers utilized in chromosome sorting were evaluated for potential effects on maintenance of chromosomal proteins during sorting. The data indicate that the presence of several buffer constituents results in the extraction of all but a few chromosomal proteins. The subsequent use of a magnesium sulfate buffer resulted in the sorting of mouse chromosomes that do not suffer a significant loss of proteins. Several DNA stains were also evaluated for causing protein dissociation, but no significant losses were observed. Although flow-sorted chromosomes have been used extensively for DNA analysis and cloning, this is a pioneering effort by the NFCR, and its collaborators, to exploit chromosome sorting capabilities for the analysis of chromosomal proteins.
Date: December 31, 1998
Creator: Hamkalo, B.A.; Henschen, A. & Parseghian, M.H.
Partner: UNT Libraries Government Documents Department

Seismic and Rockphysics Diagnostics of Multiscale Reservoir Textures

Description: This final technical report summarizes the results of the work done in this project. The main objective was to quantify rock microstructures and their effects in terms of elastic impedances in order to quantify the seismic signatures of microstructures. Acoustic microscopy and ultrasonic measurements were used to quantify microstructures and their effects on elastic impedances in sands and shales. The project led to the development of technologies for quantitatively interpreting rock microstructure images, understanding the effects of sorting, compaction and stratification in sediments, and linking elastic data with geologic models to estimate reservoir properties. For the public, ultimately, better technologies for reservoir characterization translates to better reservoir development, reduced risks, and hence reduced energy costs.
Date: July 1, 2005
Creator: Mavko, Gary
Partner: UNT Libraries Government Documents Department

Sorting fluorescent nanocrystals with DNA

Description: Semiconductor nanocrystals with narrow and tunable fluorescence are covalently linked to oligonucleotides. These biocompounds retain the properties of both nanocrystals and DNA. Therefore, different sequences of DNA can be coded with nanocrystals and still preserve their ability to hybridize to their complements. We report the case where four different sequences of DNA are linked to four nanocrystal samples having different colors of emission in the range of 530-640 nm. When the DNA-nanocrystal conjugates are mixed together, it is possible to sort each type of nanoparticle using hybridization on a defined micrometer -size surface containing the complementary oligonucleotide. Detection of sorting requires only a single excitation source and an epifluorescence microscope. The possibility of directing fluorescent nanocrystals towards specific biological targets and detecting them, combined with their superior photo-stability compared to organic dyes, opens the way to improved biolabeling experiments, such as gene mapping on a nanometer scale or multicolor microarray analysis.
Date: December 10, 2001
Creator: Gerion, Daniele; Parak, Wolfgang J.; Williams, Shara C.; Zanchet, Daniela; Micheel, Christine M. & Alivisatos, A. Paul
Partner: UNT Libraries Government Documents Department


Description: The large acceptance and compact size of the Spallation Neutron Source (SNS) ring implies the use of short, large aperture dipole magnets, with significant end field errors. The SNS will contain 32 such dipoles. We report magnetic field measurements of the first 16 magnets. The end field errors have been successfully compensated by the use of iron bumps. For 1.0 GeV protons, the magnets have been shimmed to meet the 0.01% specification for rms variation of the integral field. At 1.3 GeV, the rms variation is 0.036%. The load on the corrector system at 1.3 GeV will be reduced by the use of sorting.
Date: June 3, 2002
Creator: Wanderer, P.; Jackson, J.; Jain, A.; Lee, Y. Y.; Meng, W.; Papaphilippou, I. et al.
Partner: UNT Libraries Government Documents Department

Experience with measuring magnetic moments of permanent magnet blocks at Lawrence Berkeley Laboratory

Description: Since May 1985, The Magnetic Measurements Engineering Group at LBL has measured and sorted a total of 3834 permanent magnet blocks. These magnetic blocks have been used in the construction of various successful beam-line elements including dipoles, quadrupoles, and wigglers. We report on observed variations in magnetic moments among blocks supplied by five manufacturers, describe the operational capabilities (accuracy, precision, and resolution) of the LBL Magnetic-moment Measurement and Sorting System (MMSS), cite the results of comparative calibrations by permanent-magnet manufacturers and other National Laboratories, and suggest criteria for automating the MMSS for measuring the large number of permanent-magnet blocks required for the insertion devices for the projected LBL 1-2 GeV Synchrotron Radiation Source. 14 refs., 2 figs., 2 tabs.
Date: September 1, 1987
Creator: Nelson, D.H.; Barale, P.J.; Green, M.I. & Van Dyke, D.A.
Partner: UNT Libraries Government Documents Department


Description: This report describes work conducted on the Building Materials Reclamation Program for the period of September 2008 to August 2010. The goals of the project included selecting materials from the local construction and demolition (C&D) waste stream and developing economically viable reprocessing, reuse or recycling schemes to divert them from landfill storage. Educational resources as well as conceptual designs and engineering feasibility demonstrations were provided for various aspects of the work. The project was divided into two distinct phases: Research and Engineering Feasibility and Dissemination. In the Research Phase, a literature review was initiated and data collection commenced, an advisory panel was organized, and research was conducted to evaluate high volume C&D materials for nontraditional use; five materials were selected for more detailed investigations. In the Engineering Feasibility and Dissemination Phase, a conceptual study for a regional (Mecklenburg and surrounding counties) collection and sorting facility was performed, an engineering feasibility project to demonstrate the viability of recycling or reuse schemes was created, the literature review was extended and completed, and pedagogical materials were developed. Over the two-year duration of the project, all of the tasks and subtasks outlined in the original project proposal have been completed. The Final Progress Report, which briefly describes actual project accomplishments versus the tasks/subtasks of the original project proposal, is included in Appendix A of this report. This report describes the scientific/technical aspects (hypotheses, research/testing, and findings) of six subprojects that investigated five common C&D materials. Table 1 summarizes the six subprojects, including the C&D material studied and the graduate student and the faculty advisor on each subproject.
Date: August 31, 2010
Creator: Weggel, David C.; Chen, Shen-En; Hilger, Helene; Besnard, Fabien; Cavalline, Tara; Tempest, Brett et al.
Partner: UNT Libraries Government Documents Department