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Extension of the Southern Hemisphere Atmospheric Radiocarbon Curve, 2120-850 years BP: Results from Tasmanian Huon Pine

Description: Decadal samples of dendrochronologically-dated pine (Lagorostrobos franklinii) from the Stanley River basin, Tasmania have been radiocarbon dated between 2120-850 yr BP. This data set overlaps and extends the current Southern Hemisphere record, which currently covers the period 110-995 yr BP. There is good agreement between the two records between 995-850 yr BP, between sample replicates and with consensus values for standards. As in the younger dataset, we find evidence for a distinct but variable offset between the southern hemisphere data and IntCal04; although this is likely due to real temporal variability in the interhemispheric offset, further work is planned to rule out possible laboratory or sample preparation differences.
Date: February 12, 2010
Creator: Zimmerman, S R; P.Guilderson, T; Buckley, B M & Cook, E
Partner: UNT Libraries Government Documents Department

Loss of Radioactivity from Barium Carbonate Samples

Description: The exchange loss of radioactivity from solid samplos of barium carbonate through the agency of carbonic acid and its ions has been investigated under a variety of conditions. It is concluded that the losses observed are greatly dependent upon the method of sample preparation, in particular the nature or any heat treatment which the sample materials undergo.
Date: January 9, 1948
Creator: Yankwich, Peter E.
Partner: UNT Libraries Government Documents Department

DC Field Emission Studies on Nb

Description: The DC field emission studies in TJNAF aim to further explore the causes of field emission from Niobium surfaces and enhance our understanding of various preparation techniques for the purpose of improving field emission suppression. Field emitters on Nb samples are located by a DC field emission scanning apparatus in ultra high vacuum, then individually studied under SEM with EDS for characterization. Results at above 100 MV/m from samples prepared by BCP, and by electropolishing are presented and compared.
Date: September 1, 2001
Creator: Wang, T.; Reece, C. & Sundelin, R.
Partner: UNT Libraries Government Documents Department

Soft X-ray synchrotron radiation investigations of actinidematerials systems utilizing X-ray emission spectroscopy and resonantinelastic X-ray scattering

Description: Synchrotron radiation (SR) methods have been utilized with increasing frequency over the past several years to study topics in actinide science, ranging from those of a fundamental nature to those that address a specifically-targeted technical need. In particular, the emergence of microspectroscopic and fluorescence-based techniques have permitted investigations of actinide materials at sources of soft x-ray SR. Spectroscopic techniques with fluorescence-based detection are useful for actinide investigations since they are sensitive to small amounts of material and the information sampling depth may be varied. These characteristics also serve to simplify both sample preparation and safety considerations. Examples of investigations using these fluorescence techniques will be described along with their results, as well as the prospects for future investigations utilizing these methodologies.
Date: January 3, 2004
Creator: Shuh, D.K.; Butorin, S.M.; Guo, J.-H. & Nordgren, J.
Partner: UNT Libraries Government Documents Department

Mechanical and Electrical Properties of CdTe Tetrapods Studied byAtomic Force Microscopy

Description: The mechanical and electrical properties of CdTe tetrapod-shaped nanocrystals have been studied with atomic force microscopy. Tapping mode images of tetrapods deposited on silicon wafers revealed that they contact the surface with the ends of three arms. The length of these arms was found to be 130 {+-} 10 nm. A large fraction of the tetrapods had a shortened vertical arm as a result of fracture during sample preparation. Fracture also occurs when the applied load is a few nanonewtons. Compression experiments with the AFM tip indicate that tetrapods with the shortened vertical arm deform elastically when the applied force was less than 50 nN. Above 90 nN additional fracture events occurred that further shorted the vertical arm. Loads above 130 nN produced irreversible damage to the other arms as well. Current-voltage characteristics of tetrapods deposited on gold indicated semiconducting behavior with a current gap of {approx}2 eV at low loads (<50 nN) and a narrowing to about 1 eV at loads between 60 and 110 nN. Atomic calculation of the deformation suggests that the ends of the tetrapod arms are stuck during compression so that the deformations are due to bending modes. The reduction of the current gap is due to electrostatic effects, rather than strain deformation effects inside the tetrapod.
Date: August 30, 2007
Creator: Fang, Liang; Park, Jeong Young; Cui, Yi; Alivisatos, Paul; Shcrier, Joshua; Lee, Byounghak et al.
Partner: UNT Libraries Government Documents Department

Field Deployable DNA analyzer

Description: This report details the feasibility of a field deployable DNA analyzer. Steps for swabbing cells from surfaces and extracting DNA in an automatable way are presented. Since enzymatic amplification reactions are highly sensitive to environmental contamination, sample preparation is a crucial step to make an autonomous deployable instrument. We perform sample clean up and concentration in a flow through packed bed. For small initial samples, whole genome amplification is performed in the packed bed resulting in enough product for subsequent PCR amplification. In addition to DNA, which can be used to identify a subject, protein is also left behind, the analysis of which can be used to determine exposure to certain substances, such as radionuclides. Our preparative step for DNA analysis left behind the protein complement as a waste stream; we determined to learn if the proteins themselves could be analyzed in a fieldable device. We successfully developed a two-step lateral flow assay for protein analysis and demonstrate a proof of principle assay.
Date: February 9, 2005
Creator: Wheeler, E; Christian, A; Marion, J; Sorensen, K; Arroyo, E; Vrankovich, G et al.
Partner: UNT Libraries Government Documents Department

Traceable Micro-Force Sensor for Instrumented Indentation Calibration

Description: Instrumented indentation testing (IIT), commonly referred to as nanoindentation when small forces are used, is a popular technique for determining the mechanical properties of small volumes of material. Sample preparation is relatively easy, usually requiring only that a smooth surface of the material to be tested be accessible to a contact probe, and instruments that combine sophisticated automation with straightforward user interfaces are available commercially from several manufacturers. In addition, documentary standards are now becoming available from both the International Standards Organization (ISO 14577) and ASTM International (E28 WK382) that define test methods and standard practices for IIT, and will allow the technique to be used to produce material property data that can be used in product specifications. These standards also define the required level of accuracy of the force data produced by IIT instruments, as well as methods to verify that accuracy. For forces below 10 mN, these requirements can be difficult to meet, particularly for instrument owners who need to verify the performance of their instrument as it is installed at their site. In this paper, we describe the development, performance and application of an SI-traceable force sensor system for potential use in the field calibration of commercial IIT instruments. The force sensor itself, based on an elastically deforming capacitance gauge, is small enough to mount in a commercial instrument as if it were a test specimen, and is used in conjunction with an ultra-high accuracy capacitance bridge. The sensor system is calibrated with NIST-traceable masses over the range 5.0 {micro}N through 5.0 mN. We will present data on its accuracy and precision, as well its potential application to the verification of force in commercial instrumented indentation instruments.
Date: April 2, 2007
Creator: Smith, D T; Shaw, G A; Seugling, R M; Xiang, D & Pratt, J R
Partner: UNT Libraries Government Documents Department

Beryllium Sampling and Analysis Within the DOE Complex and Opportunities for Standardization

Description: Since the U. S. Department of Energy (DOE) published the DOE Beryllium Rule (10 CFR 850) in 1999, DOE sites have been required to measure beryllium on air filters and wipes for worker protection and for release of materials from beryllium-controlled areas. Measurements in the nanogram range on a filter or wipe are typically required. Industrial hygiene laboratories have applied methods from various analytical compendia, and a number of issues have emerged with sampling and analysis practices. As a result, a committee of analytical chemists, industrial hygienists, and laboratory managers was formed in November 2003 to address the issues. The committee developed a baseline questionnaire and distributed it to DOE sites and other agencies in the U.S. and U.K. The results of the questionnaire are presented in this paper. These results confirmed that a wide variety of practices were in use in the areas of sampling, sample preparation, and analysis. Additionally, although these laboratories are generally accredited by the American Industrial Hygiene Association (AIHA), there are inconsistencies in performance among accredited labs. As a result, there are significant opportunities for development of standard methods that could improve consistency. The current availabilities and needs for standard methods are further discussed in a companion paper.
Date: January 25, 2005
Creator: BRISSON, MICHAEL
Partner: UNT Libraries Government Documents Department

Support for Synchrotron Access by Environmental Scientists

Description: To support ERSP-funded scientists in all aspects of synchrotron-based research at the Advanced Photon Source (APS). This support comes in one or more of the following forms: (1) writing proposals to the APS General User (GU) program, (2) providing time at MRCAT/EnviroCAT beamlines via the membership of the Molecular Environmental Science (MES) Group in MRCAT/EnviroCAT, (3) assistance in experimental design and sample preparation, (4) support at the beamline during the synchrotron experiment, (5) analysis and interpretation of the synchrotron data, and (6) integration of synchrotron experimental results into manuscripts.
Date: June 1, 2006
Creator: Daly, Michael; Madden, Andrew; Palumbo, Anthony & Qafoku, N.
Partner: UNT Libraries Government Documents Department

NEW METHOD FOR REMOVAL OF SPECTRAL INTERFERENCES FOR BERYLLIUM ASSAY USING INDUCTIVELY COUPLED PLASMA ATOMIC EMISSION SPECTROMETRY

Description: Beryllium has been used widely in specific areas of nuclear technology. Frequent monitoring of air and possible contaminated surfaces in U.S Department of Energy (DOE) facilities is required to identify potential health risks and to protect DOE workers from beryllium-contaminated dust. A new method has been developed to rapidly remove spectral interferences prior to beryllium (Be) measurement by inductively-coupled plasma atomic emission spectrometry (ICP-AES). The ion exchange separation removes uranium (U), thorium (Th), niobium (Nb), vanadium (V), molybdenum (Mo), zirconium (Zr), tungsten (W), iron (Fe), chromium (Cr), cerium (Ce), erbium (Er) and titanium (Ti). A stacked column consisting of Diphonix Resin{reg_sign} and TEVA Resin{reg_sign} reduces the levels of the spectral interferences so that low level Be measurements can be performed accurately. If necessary, an additional anion exchange separation can be used for further removal of interferences, particularly chromium. The method has been tested using spiked filters, spiked wipe samples and certified reference material standards with high levels of interferences added. The method provides very efficient removal of spectral interferences with very good accuracy and precision for beryllium on filters or wipes. A vacuum box system is employed to reduce analytical time and reduce labor costs.
Date: January 14, 2008
Creator: Maxwell, S; Matthew Nelson, M; Linda Youmans, L & Maureen Bernard, M
Partner: UNT Libraries Government Documents Department

ELECTRON AMPLIFICATION IN DIAMOND.

Description: We report on recent progress toward development of secondary emission ''amplifiers'' for photocathodes. Secondary emission gain of over 300 has been achieved in transmission mode and emission mode for a variety of diamond samples. Techniques of sample preparation, including hydrogenation to achieve negative electron affinity (NEA), have been adapted to this application.
Date: July 10, 2006
Creator: SMEDLEY, J.; BEN-ZVI, I.; BURRILL, A.; CHANG, X.; GRIMES, J.; RAO, T. et al.
Partner: UNT Libraries Government Documents Department

Normal and tumor-derived myoepithelial cells differ in their ability to interact with luminal breast epithelial cells for polarity and basement membrane deposition

Description: The signals that determine the correct polarity of breast epithelial structures in vivo are not understood. We have shown previously that luminal epithelial cells can be polarized when cultured within a reconstituted basement membrane gel. We reasoned that such cues in vivo may be given by myoepithelial cells. Accordingly, we used an assay where luminal epithelial cells are incorrectly polarized to test this hypothesis. We show that culturing human primary luminal epithelial cells within collagen-I gels leads to formation of structures with no lumina and with reverse polarity as judged by dual stainings for sialomucin, epithelial specific antigen or occludin. No basement membrane is deposited, and {beta}4-integrin staining is negative. Addition of purified human myoepithelial cells isolated from normal glands corrects the inverse polarity, and leads to formation of double-layered acini with central lumina. Among the laminins present in the human breast basement membrane (laminin-1, -5 and -10/11), laminin-1 was unique in its ability to substitute for myoepithelial cells in polarity reversal. Myoepithelial cells were purified also from four different breast cancer sources including a biphasic cell line. Three out of four samples either totally lacked the ability to interact with luminal epithelial cells, or conveyed only correction of polarity in a fraction of acini. This behavior was directly related to the ability of the tumor myoepithelial cells to produce {alpha}-1 chain of laminin. In vivo, breast carcinomas were either negative for laminin-1 (7/12 biopsies) or showed a focal, fragmented deposition of a less intensely stained basement membrane (5/12 biopsies). Dual staining with myoepithelial markers revealed that tumorassociated myoepithelial cells were either negative or weakly positive for expression of laminin-1, establishing a strong correlation between loss of laminin-1 and breast cancer. We conclude that the double-layered breast acinus may be recapitulated in culture and that one reason for the ability ...
Date: October 4, 2001
Creator: Gudjonsson, Thorarinn; Ronnov-Jessen, Lone; Villadsen, Rene; Rank, Fritz; Bissell, Mina J. & Petersen, Ole William
Partner: UNT Libraries Government Documents Department

Laser: a Tool for Optimization and Enhancement of Analytical Methods

Description: In this work, we use lasers to enhance possibilities of laser desorption methods and to optimize coating procedure for capillary electrophoresis (CE). We use several different instrumental arrangements to characterize matrix-assisted laser desorption (MALD) at atmospheric pressure and in vacuum. In imaging mode, 488-nm argon-ion laser beam is deflected by two acousto-optic deflectors to scan plumes desorbed at atmospheric pressure via absorption. All absorbing species, including neutral molecules, are monitored. Interesting features, e.g. differences between the initial plume and subsequent plumes desorbed from the same spot, or the formation of two plumes from one laser shot are observed. Total plume absorbance can be correlated with the acoustic signal generated by the desorption event. A model equation for the plume velocity as a function of time is proposed. Alternatively, the use of a static laser beam for observation enables reliable determination of plume velocities even when they are very high. Static scattering detection reveals negative influence of particle spallation on MS signal. Ion formation during MALD was monitored using 193-nm light to photodissociate a portion of insulin ion plume. These results define the optimal conditions for desorbing analytes from matrices, as opposed to achieving a compromise between efficient desorption and efficient ionization as is practiced in mass spectrometry. In CE experiment, we examined changes in a poly(ethylene oxide) (PEO) coating by continuously monitoring the electroosmotic flow (EOF) in a fused-silica capillary during electrophoresis. An imaging CCD camera was used to follow the motion of a fluorescent neutral marker zone along the length of the capillary excited by 488-nm Ar-ion laser. The PEO coating was shown to reduce the velocity of EOF by more than an order of magnitude compared to a bare capillary at pH 7.0. The coating protocol was important, especially at an intermediate pH of 7.7. The increase of ...
Date: January 1, 1997
Creator: Preisler, Jan
Partner: UNT Libraries Government Documents Department

Platinum Catalyzed Decomposition of Activated Carbon: 1. Initial Studies

Description: Carbon is an important support for heterogeneous catalysts, such as platinum supported on activated carbon (AC). An important property of these catalysts is that they decompose upon heating in air. Consequently, Pt/AC catalysts can be used in applications requiring rapid decomposition of a material, leaving little residue. This report describes the catalytic effects of platinum on carbon decomposition in an attempt to maximize decomposition rates. Catalysts were prepared by impregnating the AC with two different Pt precursors, Pt(NH{sub 3}){sub 4}(NO{sub 3}){sub 2} and H{sub 2}PtCl{sub 6}. Some catalysts were treated in flowing N{sub 2} or H{sub 2} at elevated temperatures to decompose the Pt precursor. The catalysts were analyzed for weight loss in air at temperatures ranging from 375 to 450 C, using thermogravimetric analysis (TGA). The following results were obtained: (1) Pt/AC decomposes much faster than pure carbon; (2) treatment of the as-prepared 1% Pt/AC samples in N{sub 2} or H{sub 2} enhances decomposition; (3) autocatalytic behavior is observed for 1% Pt/AC samples at temperatures {ge} 425 C; (4) oxygen is needed for decomposition to occur. Overall, the Pt/AC catalyst with the highest activity was impregnated with H{sub 2}PtCl{sub 6} dissolved in acetone, and then treated in H{sub 2}. However, further research and development should produce a more active Pt/AC material.
Date: December 1, 2001
Creator: MUDD, JASON E.; GARDNER, TIMOTHY J. & SAULT, ALLEN G.
Partner: UNT Libraries Government Documents Department

Evaluation of kinetic phosphorescence analysis for the determination of uranium

Description: In the past, New Brunswick Laboratory (NBL) has used a fluorometric method for the determination of sub-microgram quantities of uranium. In its continuing effort to upgrade and improve measurement technology, NBL has evaluated the commercially-available KPA-11 kinetic phosphorescence analyzer (Chemchek, Richland, WA). The Chemchek KPA-11 is a bench-top instrument which performs single-measurement, quench-corrected analyses for trace uranium. It incorporates patented kinetic phosphorimetry techniques to measure and analyze sample phosphorescence as a function of time. With laser excitation and time-corrected photon counting, the KPA-11 has a lower detection limit than conventional fluorometric methods. Operated with a personal computer, the state-of-the-art KPA-11 offers extensive time resolution and phosphorescence lifetime capabilities for additional specificity. Interferences are thereby avoided while obtaining precise measurements. Routine analyses can be easily and effectively accomplished, with the accuracy and precision equivalent to the pulsed-laser fluorometric method presently performed at NBL, without the need for internal standards. Applications of kinetic phosphorimetry at NBL include the measurement of trace level uranium in retention tank, waste samples, and low-level samples. It has also been used to support other experimental activities at NBL by the measuring of nanogram amounts of uranium contamination (in blanks) in isotopic sample preparations, and the determining of elution curves of different ion exchange resins used for uranium purification. In many cases, no pretreatment of samples was necessary except to fume them with nitric acid, and then to redissolve and dilute them to an appropriate concentration with 1 M HNO{sub 3} before measurement. Concentrations were determined on a mass basis ({micro}g U/g of solution), but no density corrections were needed since all the samples (including the samples used for calibration) were in the same density matrix (1 M HNO{sub 3}). A statistical evaluation of the determination of uranium using kinetic phosphorimetry is described in this report, along with ...
Date: December 1, 1997
Creator: Croatto, P.V.; Frank, I.W.; Johnson, K.D.; Mason, P.B. & Smith, M.M.
Partner: UNT Libraries Government Documents Department