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Screening of Steroids from Plants Using Saccharomyces Cerevisiae as a Host System

Description: The reconstituted steroid transcription unit in Saccharomyces cerevisae was used to screen for steroids in cell extracts prepared from 24 species of plants representing 16 families. Extracts from 7 species representing 5 families were able to activate both estrogen and progesterone receptors expressed in yeast.
Date: August 1992
Creator: Maier, Camelia G. A. (Camelia Gabriela-Anca)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Competitive repair by naturally dispersed repetitive DNA during non-allelic homologous recombination

Description: Genome rearrangements often result from non-allelic homologous recombination (NAHR) between repetitive DNA elements dispersed throughout the genome. Here we systematically analyze NAHR between Ty retrotransposons using a genome-wide approach that exploits unique features of Saccharomyces cerevisiae purebred and Saccharomyces cerevisiae/Saccharomyces bayanus hybrid diploids. We find that DNA double-strand breaks (DSBs) induce NAHR-dependent rearrangements using Ty elements located 12 to 48 kilob… more
Date: August 27, 2010
Creator: Hoang, Margaret L.; Tan, Frederick J.; Lai, David C.; Celniker, Sue E.; Hoskins, Roger A.; Dunham, Maitreya J. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Regulation of Pyridine Nucleotide Metabolism in Saccharomyces cerevisiae

Description: The levels of total nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP), and their redox states were determined as the function of growth in S. cerevisiae. Cells growing in a medium containing 0.8% glucose exhibit two phases of exponential growth, utilizing glucose and ethanol, respectively. The NAD pool is 50% reduced during both stages of growth while the NADP pool is 67% reduced in glucose growth and 48% reduced in ethanol growth. The NAD/NADP ratio… more
Date: May 1976
Creator: Ting, Haung-yu
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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Detection of Topological Patterns in Protein Networks.

Description: Complex networks appear in biology on many different levels: (1) All biochemical reactions taking place in a single cell constitute its metabolic network, where nodes are individual metabolites, and edges are metabolic reactions converting them to each other. (2) Virtually every one of these reactions is catalyzed by an enzyme and the specificity of this catalytic function is ensured by the key and lock principle of its physical interaction with the substrate. Often the functional enzyme is for… more
Date: November 17, 2003
Creator: Maslov, S. & Sneppen, K.
Item Type: Refine your search to only Book
Partner: UNT Libraries Government Documents Department
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2010 CELL AND MOLECULAR FUNGAL BIOLOGY GORDON RESEARCH CONFERENCE, JUNE 13-18, 2010

Description: The Cellular and Molecular Fungal Biology Conference provides a forum for presentation of the latest advances in fungal research with an emphasis on filamentous fungi. This open-registration scientific meeting brings together the leading scientists from academia, government and industry to discuss current research results and future directions at Holderness School, an outstanding venue for scientific interaction. A key objective of the conference is to foster interaction among scientists workin… more
Date: June 18, 2010
Creator: Momany, Michelle
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Transformation-associated recombination between diverged and homologous DNA repeats is induced by strand breaks

Description: Rearrangement and deletion within plasmid DNA is commonly observed during transformation. We have examined the mechanisms of transformation-associated recombination in the yeast Saccharomyces cerevisiae using a plasmid system which allowed the effects of physical state and/or extent of homology on recombination to be studied. The plasmid contains homologous or diverged (19%) DNA repeats separated by a genetically detectable color marker. Recombination during transformation for covalently closed… more
Date: October 1, 1994
Creator: Larionov, V.; Kouprina, N.; Eldarov, M.; Perkins, E.; Porter, G. & Resnick, M. A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

DNA sequencing conference, 2

Description: This conference focused on DNA sequencing, genetic linkage mapping, physical mapping, informatics and bioethics. Several were used to study this sequencing and mapping. This article also discusses computer hardware and software aiding in the mapping of genes.
Date: June 19, 1991
Creator: Cook-Deegan, R. M.; Venter, J. C.; Gilbert, W.; Mulligan, J. & Mansfield, B. K.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol

Description: BackgroundIncreasing energy costs and environmental concerns have motivated engineering microbes for the production of ?second generation? biofuels that have better properties than ethanol.Results& ConclusionsSaccharomyces cerevisiae was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms (S. cerevisiae, Escherichia coli, Clostridium beijerinckii, and Ralstonia eutropha) were substituted for the Clostridial enzymes and their effect on n-… more
Date: November 25, 2008
Creator: Steen, EricJ.; Chan, Rossana; Prasad, Nilu; Myers, Samuel; Petzold, Christopher; Redding, Alyssa et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
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Position specific variation in the rate of evolution intranscription factor binding sites

Description: The binding sites of sequence specific transcription factors are an important and relatively well-understood class of functional non-coding DNAs. Although a wide variety of experimental and computational methods have been developed to characterize transcription factor binding sites, they remain difficult to identify. Comparison of non-coding DNA from related species has shown considerable promise in identifying these functional non-coding sequences, even though relatively little is known about … more
Date: August 28, 2003
Creator: Moses, Alan M.; Chiang, Derek Y.; Kellis, Manolis; Lander, EricS. & Eisen, Michael B.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Population genetic variation in gene expression is associated withphenotypic variation in Saccharomyces cerevisiae

Description: The relationship between genetic variation in gene expression and phenotypic variation observable in nature is not well understood. Identifying how many phenotypes are associated with differences in gene expression and how many gene-expression differences are associated with a phenotype is important to understanding the molecular basis and evolution of complex traits. Results: We compared levels of gene expression among nine natural isolates of Saccharomyces cerevisiae grown either in the prese… more
Date: February 25, 2004
Creator: Fay, Justin C.; McCullough, Heather L.; Sniegowski, Paul D. & Eisen, Michael B.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Characterization of a New Family of Metal Transporters

Description: Metal ions are critical nutrients, yet overaccumulation of these same metals can also be toxic. To maintain appropriate intracellular levels, cells require specific metal uptake systems that are subject to precise homeostatic regulation. The long-range goal of our research is to define the molecular mechanism(s) and regulation of metal ion uptake in eukaryotic cells. Integrating genetic, molecular biological and biochemical approaches, we have examined these processes in the yeast Saccharomyces… more
Date: April 29, 2002
Creator: Geurinot, Mary Lou & Eide, David
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Performance of co-immobilized yeast and glucoamylase in a fluidized bed reactor for fuel ethanol production

Description: The performance of co-immobilized Saccharomyces cerevisiae and glucoamylase was evaluated in a fluidized bed reactor. Soluble starch and yeast extract were used as feed stocks. The biocatalyst performed well and demonstrated no significant loss of activity or physical integrity during 10 weeks of continuous operation. The reactor was easily operated and required no pH control. No operational problems were encountered from bacterial contaminants even though the reactor was operated under non-ste… more
Date: July 1, 1996
Creator: Sun, M. Y.; Bienkowski, P. R.; Davison, B. H.; Spurrier, M. A. & Webb, O. F.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Deletions, duplications and transpositions of the COR segment that encompasses the structural gene of yeast iso-1-cytochrome c

Description: It has been recently found that a specific chromosomal segment, in certain but not all laboratory strains of Saccharomyces cerevisiae, is deleted and transposed at high frequencies. This segment, denoted COR, encompasses the three closely linked loci CYC1, OSM1 and RAD7 which control iso-1-cytochrome c, osmotic sensitivity and UV-sensitivity, respectively. Two types of apparently normal laboratory strains of yeast designated COR1 and COR2, were uncovered after the examination of the frequencies… more
Date: January 1, 1980
Creator: Stiles, John I.; Friedman, Linda R. & Sherman, Fred
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Yeast mutants overproducing iso-cytochromes c

Description: For over 15 years, the iso-cytochrome c system in the yeast Saccharomyces cerevisiae has been used to investigate a multitude of problems in genetics and molecular biology. More recently, attention has been focused on using mutants for examining translation and transcriptional processes and for probing regulatory regions governing gene expression. In an effort to explore regulatory mechanisms and to investigate mutational alterations that lead to increased levels of gene products, we have isola… more
Date: January 1, 1980
Creator: Sherman, F.; Cardillo, T. S.; Errede, B.; Friedman, L.; McKnight, G. & Stiles, J. I.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Transformation-associated recombination between diverged and homologous DNA repeats is induced by strand breaks

Description: Rearrangement and deletion within plasmid DNA is commonly observed during transformation. We have examined the mechanisms of transformation-associated recombination in the yeast Saccharomyces cerevisiae using a plasmid system which allowed the effects of physical state and/or extent of homology on recombination to be studied. The plasmid contains homologous or diverged (19%) DNA repeats separated by a genetically detectable color marker. Recombination during transformation for covalently closed… more
Date: December 31, 1993
Creator: Larionov, V.; Kouprina, N.; Edlarov, M.; Perkins, E.; Porter, G. & Resnick, M. A.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

ROAM mutations causing increased expression of yeast genes: their activation by signals directed toward conjugation functions and their formation by insertion of tyl repetitive elements

Description: Mechanisms available to eukaryotic organisms for the coordinate regulation of gene expression are being examined by genetic and biochemical characterization of an unusual mutation, CYC7-H2, which causes overproduction of iso-2-cytochrome c in the yeast Saccharomyces cerevisiae. The CYC7-H2 mutation causes approximately a twenty fold overproduction of iso-2-cytochrome c in haploid strains but only a one to four fold overproduction in MATa/MAT..cap alpha.. diploid strains. This regulation of over… more
Date: January 1, 1980
Creator: Errede, Beverly; Cardillo, Thomas S.; Wever, Grace & Sherman, Fred
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Transport function and reaction mechanism of vacuolar H{sup +}- translocation inorganic pyrophosphatase. Annual technical progress report

Description: These studies describe progress made in the definition of the transport capabilities and reaction mechanism of the V-PPase through biochemical analyses of native membrane vesicles isolated from etiolated hypocotyls of Vigna radiate and by reconstitution of the purified enzyme into artificial liposomes; delineation of the ligand requirements of the V-PPase; and the delineation of sequence motifs implicated in substrate-binding through the development of strategies for selective cleavage of the M… more
Date: September 1, 1993
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Characterization of a new family of metal transporters

Description: Metal ions are critical nutrients, yet overaccumulation of these same metals can also be toxic. To maintain appropriate intracellular levels, cells require specific metal uptake systems that are subject to precise homeostatic regulation. The long-range goal of our research is to define the molecular mechanism(s) and regulation of metal ion uptake in eukaryotic cells. Integrating genetic, molecular biological and biochemical approaches, we have examined these processes in the yeast Saccharomyces… more
Date: December 31, 1999
Creator: Guerinot, Mary L. & Dide, David
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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