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Two-dimensional gel electrophoresis of in vivo and in vitro synthesized proteins, antigenic proteins, and cross-reactive antigens in Treponema pallidum subsp. pallidum Nichols strain and Treponema phagedenis biotype Reiter

Description: Two-dimensional electrophoretic protein profiles of in vivo and in vitro propagated T. pallidum subsps. pallidum Nichols strain were analyzed and compared.
Date: May 1989
Creator: Sayahtaheri, Sousan
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries

How to Select Foods: [Part] 3. Foods Rich in Protein

Description: "This bulletin deals with food materials which are rich in protein and tells why the body needs this nutrient and how much is supplied by different foods. Since the protein foods include many of the more expensive foods in common use, and since an adequate supply of protein is essential to the growth and upkeep of the body, it is especially important for the housekeeper to know how much her family needs and to be able to choose the materials which, in her particular circumstances, will best provide the proper kind and amount." -- p. 2
Date: 1917
Creator: Hunt, Caroline Louisa, 1865-1927 & Atwater, Helen W.
Item Type: Refine your search to only Pamphlet
Partner: UNT Libraries Government Documents Department

Proposed Import Restrictions on Milk Protein Concentrates (MPCs)

Description: Low farm milk prices and declining dairy sector income in 2009 have renewed congressional interest in imposing new import barriers on milk protein concentrates (MPCs), which generally include casein, the main protein found in milk, and caseinates, a soluble form of casein. This report examines proposed import restrictions and potential trade policy implications.
Date: September 30, 2009
Creator: Shields, Dennis A. & Hanrahan, Charles E.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department

Proposed Import Restrictions on Milk Protein Concentrates (MPCs)

Description: Low farm milk prices and declining dairy sector income in 2009 have renewed congressional interest in imposing new import barriers on milk protein concentrates (MPCs), which generally include casein, the main protein found in milk, and caseinates, a soluble form of casein. This report examines proposed import restrictions and potential trade policy implications.
Date: January 12, 2010
Creator: Shields, Dennis A. & Hanrahan, Charles E.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department

Investigation of the developmental profile of chromosomal proteins in Zea mays

Description: Histone proteins were examined during development in the meiotic elongate and a genetically close line N6HT. Histones were also extracted from an F1 (el x N6Ht). Comparisonc beteen the histone samples from each line of N6HT and elongate, and the F1 for leaf, root, and stem were inconclusive. A tassel sample form elongate exhibited a markedly slower migrating band that was not present in N6HT. The histone profiles of elongate and N6HT also differed. Each line N6HT and elongate exhibited three protein bands in the H1 region. Maize histone samples have been shown to exhibit four major H1 bands. The possibility exists that an H1 protein altered in its molecular weight and possibly in its interaction with the chromosome is present in elongate.
Date: May 1985
Creator: Jordan, Berry Lyn
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries

Protein profiles of azotobacter vinelandii during the encystment process

Description: Azobacter vinelandii 12837 was grown in Burk's glucose media and transferred onto Burk's n-butanol agar plates to allow for the formation of cysts. The patterns of the vegetative cell proteins were compared for each successive day of cyst formation, using the polycrylamide gel isoelectric focusing technique.
Date: August 1977
Creator: Butler, Mark A.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries

Identification of Endogenous Substrates for ADP-Ribosylation in Rat Liver

Description: Bacterial toxins have been shown to modify animal cell proteins in vivo with ADPR. Animal cells also contain endogenous enzymes that can modify proteins. Indirect evidence for the existence in vivo of rat liver proteins modified by ADPR on arginine residues has been reported previously. Presented here is direct evidence for the existence of ADP-ribosylarginine in rat liver proteins. Proteins were subjected to exhaustive protease digestion and ADP-ribosyl amino acids were isolated by boronate chromatography.
Date: May 1992
Creator: Loflin, Paul T. (Paul Tracey)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries

What makes species unique? The contribution of proteins with obscure features

Description: This article performs a comparative analysis of the predicted proteomes derived from 10 different sequenced genomes, including budding and fission yeast, worm, fly, mosquito, Arabidopsis, rice, mouse, rat, and human.
Date: June 27, 2006
Creator: Gollery, Martin; Harper, Jeffrey F.; Cushman, John; Mittler, Taliah; Girke, Thomas; Zhu, Jian-Kang et al.
Item Type: Refine your search to only Article
Partner: UNT College of Arts and Sciences

Phylogenetic analysis of eukaryotic NEET proteins uncovers a link between a key gene duplication event and the evolution of vertebrates

Description: This article describes the use of three members of the human NEET protein family (CISD1, mitoNEET; CISD2, NAF-1 or Miner 1; and CISD3, Miner2) as guides to conduct a phylogenetic analysis of eukaryotic NEET proteins and their evolution.
Date: February 16, 2017
Creator: Inupakutika, Madhuri A.; Sengupta, Soham; Nechushtai, Rachel; Jennings, Patricia A.; Onuchic, José N.; Azad, Rajeev K. et al.
Item Type: Refine your search to only Article
Partner: UNT College of Arts and Sciences

Interactions between mitoNEET and NAF-1 in cells

Description: This article uses yeast two-hybrid to demonstrate through vivo bimolecular fluorescence complementation (BiFC), direct coupling analysis (DCA), RNA-sequencing, ROS and iron imaging, and single and double shRNA lines with suppressed mNT, NAF-1 and mNT/NAF-1 expression, that mNT and NAF-1 directly interact in mammalian cells and could function in the same cellular pathway.
Date: April 20, 2017
Creator: Karmi, Ola; Holt, Sarah H.; Song, Luhua; Tamir, Sagi; Luo, Yuting; Bai, Fang et al.
Item Type: Refine your search to only Article
Partner: UNT College of Arts and Sciences

Integration of Phenotypic Metadata and Protein Similarity in Archaea Using a Spectral Bipartitioning Approach

Description: In order to simplify and meaningfully categorize large sets of protein sequence data, it is commonplace to cluster proteins based on the similarity of those sequences. However, it quickly becomes clear that the sequence flexibility allowed a given protein varies significantly among different protein families. The degree to which sequences are conserved not only differs for each protein family, but also is affected by the phylogenetic divergence of the source organisms. Clustering techniques that use similarity thresholds for protein families do not always allow for these variations and thus cannot be confidently used for applications such as automated annotation and phylogenetic profiling. In this work, we applied a spectral bipartitioning technique to all proteins from 53 archaeal genomes. Comparisons between different taxonomic levels allowed us to study the effects of phylogenetic distances on cluster structure. Likewise, by associating functional annotations and phenotypic metadata with each protein, we could compare our protein similarity clusters with both protein function and associated phenotype. Our clusters can be analyzed graphically and interactively online.
Date: January 1, 2009
Creator: Hooper, Sean D.; Anderson, Iain J; Pati, Amrita; Dalevi, Daniel; Mavromatis, Konstantinos & Kyrpides, Nikos C
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department

Versatile P(acman) BAC Libraries for Transgenesis Studies in Drosophila melanogaster

Description: We constructed Drosophila melanogaster BAC libraries with 21-kb and 83-kb inserts in the P(acman) system. Clones representing 12-fold coverage and encompassing more than 95percent of annotated genes were mapped onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using Phi C31 integrase to rescue mutations. They can be modified through recombineering, for example to incorporate protein tags and assess expression patterns.
Date: April 21, 2009
Creator: Venken, Koen J.T.; Carlson, Joseph W.; Schulze, Karen L.; Pan, Hongling; He, Yuchun; Spokony, Rebecca et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department

Survey of large protein complexes D. vulgaris reveals great structural diversity

Description: An unbiased survey has been made of the stable, most abundant multi-protein complexes in Desulfovibrio vulgaris Hildenborough (DvH) that are larger than Mr {approx} 400 k. The quaternary structures for 8 of the 16 complexes purified during this work were determined by single-particle reconstruction of negatively stained specimens, a success rate {approx}10 times greater than that of previous 'proteomic' screens. In addition, the subunit compositions and stoichiometries of the remaining complexes were determined by biochemical methods. Our data show that the structures of only two of these large complexes, out of the 13 in this set that have recognizable functions, can be modeled with confidence based on the structures of known homologs. These results indicate that there is significantly greater variability in the way that homologous prokaryotic macromolecular complexes are assembled than has generally been appreciated. As a consequence, we suggest that relying solely on previously determined quaternary structures for homologous proteins may not be sufficient to properly understand their role in another cell of interest.
Date: August 15, 2009
Creator: Han, B.-G.; Dong, M.; Liu, H.; Camp, L.; Geller, J.; Singer, M. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department

Functional Assessment of the Medicago truncatula NIP/LATD Protein Demonstrates That It Is a High-Affinity Nitrate Transporter

Description: Article on the functional assessment of the Medicago truncatula NIP/LATD protein demonstrating that it is a high-affinity nitrate transporter.
Date: October 2012
Creator: Bagchi, Rammyani; Salehin, Mohammad; Adeyemo, O. Sarah; Salazar, Carolina; Shulaev, Vladimir; Sherrier, D. Janine et al.
Item Type: Refine your search to only Article
Partner: UNT College of Arts and Sciences

Data growth and its impact on the SCOP database: new developments

Description: The Structural Classification of Proteins (SCOP) database is a comprehensive ordering of all proteins of known structure, according to their evolutionary and structural relationships. The SCOP hierarchy comprises the following levels: Species, Protein, Family, Superfamily, Fold and Class. While keeping the original classification scheme intact, we have changed the production of SCOP in order to cope with a rapid growth of new structural data and to facilitate the discovery of new protein relationships. We describe ongoing developments and new features implemented in SCOP. A new update protocol supports batch classification of new protein structuresby their detected relationships at Family and Superfamily levels in contrast to our previous sequential handling of new structural data by release date. We introduce pre-SCOP, a preview of the SCOP developmental version that enables earlier access to the information on new relationships. We also discuss the impact of worldwide Structural Genomics initiatives, which are producing new protein structures at an increasing rate, on the rates of discovery and growth of protein families and superfamilies. SCOP can be accessed at http://scop.mrc-lmb.cam.ac.uk/scop.
Date: November 13, 2007
Creator: Chandonia, John-Marc; Andreeva, Antonina; Howorth, Dave; Chandonia, John-Marc; Brenner, Steven E.; Hubbard, Tim J.P. et al.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department