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A Genetic Approach to Identify Proteins that Interact with Eukaryotic Microtubule Severing Proteins via a Yeast Two Hybrid System

Description: Microtubules (MT) are regulated by multiple categories of proteins, including proteins responsible for severing MTs that are therefore called MT-severing proteins. Studies of katanin, spastin, and fidgetin in animal systems have clarified that these proteins are MT-severing. However, studies in plants have been limited to katanin p60, and little is known about spastin or fidgetin and their function in plants. I looked at plant genomes to identify MT-severing protein homologues to clarify which … more
Date: May 2020
Creator: Alhassan, Hassan H
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Identification of Endogenous Substrates for ADP-Ribosylation in Rat Liver

Description: Bacterial toxins have been shown to modify animal cell proteins in vivo with ADPR. Animal cells also contain endogenous enzymes that can modify proteins. Indirect evidence for the existence in vivo of rat liver proteins modified by ADPR on arginine residues has been reported previously. Presented here is direct evidence for the existence of ADP-ribosylarginine in rat liver proteins. Proteins were subjected to exhaustive protease digestion and ADP-ribosyl amino acids were isolated by boronate ch… more
Date: May 1992
Creator: Loflin, Paul T. (Paul Tracey)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Coevolution of gene expression among interacting proteins

Description: Physically interacting proteins or parts of proteins are expected to evolve in a coordinated manner that preserves proper interactions. Such coevolution at the amino acid-sequence level is well documented and has been used to predict interacting proteins, domains, and amino acids. Interacting proteins are also often precisely coexpressed with one another, presumably to maintain proper stoichiometry among interacting components. Here, we show that the expression levels of physically interacting … more
Date: March 1, 2004
Creator: Fraser, Hunter B.; Hirsh, Aaron E.; Wall, Dennis P. & Eisen,Michael B.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Mercury-binding proteins of Mytilus edulis

Description: Mytilus edulis possesses low molecular weight, mercury-binding proteins. The predominant protein isolated from gill tissue is enriched in cysteinyl residues (8%) and possesses an amino acid composition similar to cadmium-binding proteins of mussels and oysters. Continuous exposure of mussels to 5 ..mu..g/l mercury results in spillover of mercury from these proteins to high molecular weight proteins. Antibodies to these proteins have been isolated, and development of immunoassays is presently un… more
Date: November 1, 1981
Creator: Roesijadi, G.; Morris, J.E. & Calabrese, A.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

The Stress Protein Response of Pimephales promelas to Copper

Description: Organisms synthesize stress proteins in response to a variety of stressors. The 68/70-kDa proteins (synonymous to the 72/73-kDa proteins) have shown to be the most promising stress proteins, and have been proposed as a biomarker of general organismal stress. The 68/70-kDa proteins were used in an antigen/antibody based approach to determine the duration of the stress protein response of Pimephales promelas following an acute exposure to copper sulphate.
Date: May 1992
Creator: Covington, Sean M.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Chemical Methods for the Production of Proteins

Description: The goal of this research program was to develop improved methods for chemical peptide and protein synthesis, and to apply these methods to the total synthesis of small proteins (<80 amino acids) & integral membrane proteins.
Date: September 15, 2008
Creator: Kent, Stephen B. H.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Integrated Genome-Based Studies of Shewanella Ecophysiology

Description: We have constructed in-frame deletions of 7 of the 10 PAS-GGDEF-EAL proteins in Shewanella oneidensis MR-1. We are currently in the process of characterizing the deletion mutants under a wide range of growth conditions. In addition to characterizing growth, we will also examine the biofilm formation of the deletion mutants. In addition to the genetic analyses of the mutants, we are also interested in comparing the activities of the various PAS-GGDEF-EAL proteins. Proteins containing PAS, GGDEF … more
Date: July 12, 2011
Creator: Spormann, Alfred
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Proteins in growth regulation during early development of the chick embryo. Progress report, May 1975--May 1976

Description: Previous studies have shown that the growth of embryo regions and the synthesis of specific proteins in embryo regions were altered when early chick embryos were cultured on various nutrient proteins. Because nutrient proteins were degraded to constituent amino acids within the yolk-sac and did not reach the embryo proper as intact macromolecules, we have been evaluating the role of the yolk-sac in the regulation of early embryonic growth and development. The synthesis of serum proteins by the … more
Date: April 1, 1976
Creator: Klein, N. W.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Transcription Factors in Xylem Development. Final report

Description: Answers to the following questions are answered in this report. do the two pine Byb proteins previously identified as candidate transcription factors bind to DNA and activate transcription? In what cell types are tehse Myb proteins expressed? Are these proteins localized to the nucleus? Do other proteins in pine xylem interact with these Myb proteins? Does altered expression of these genes have an impact on xylogenesis, specifically the expression of monolignol biosynthetic genes?
Date: July 1, 1999
Creator: Sederoff, Ronald; Whetten, Ross; O'Malley, David & Campbell, Malcolm
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Single proteins that serve linked functions in intracellular and extracellular microenvironments

Description: Maintenance of organ homeostasis and control of appropriate response to environmental alterations requires intimate coordination of cellular function and tissue organization. An important component of this coordination may be provided by proteins that can serve distinct, but linked, functions on both sides of the plasma membrane. Here we present a novel hypothesis in which non-classical secretion can provide a mechanism through which single proteins can integrate complex tissue functions. Singl… more
Date: June 3, 2009
Creator: Radisky, Derek C.; Stallings-Mann, Melody; Hirai, Yohei & Bissell, Mina J.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Implications of structural genomics target selection strategies: Pfam5000, whole genome, and random approaches

Description: The structural genomics project is an international effort to determine the three-dimensional shapes of all important biological macromolecules, with a primary focus on proteins. Target proteins should be selected according to a strategy which is medically and biologically relevant, of good value, and tractable. As an option to consider, we present the Pfam5000 strategy, which involves selecting the 5000 most important families from the Pfam database as sources for targets. We compare the Pfam5… more
Date: July 14, 2004
Creator: Chandonia, John-Marc & Brenner, Steven E.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Molecular dynamics of membrane proteins.

Description: Understanding the dynamics of the membrane protein rhodopsin will have broad implications for other membrane proteins and cellular signaling processes. Rhodopsin (Rho) is a light activated G-protein coupled receptor (GPCR). When activated by ligands, GPCRs bind and activate G-proteins residing within the cell and begin a signaling cascade that results in the cell's response to external stimuli. More than 50% of all current drugs are targeted toward G-proteins. Rho is the prototypical member of … more
Date: October 1, 2004
Creator: Woolf, Thomas B. (Johns Hopkins University School of Medicine, Baltimore, MD); Crozier, Paul Stewart & Stevens, Mark Jackson
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Crystallization Process of Protein Rv0731c from Mycobacterium Tuberculosis for a Successful Atomic Resolution Crystal Structure at 1.2 Angstrom

Description: Proteins are bio-macromolecules consisting of basic 20 amino acids and have distinct three-dimensional folds. They are essential parts of organisms and participate in every process within cells. Proteins are crucial for human life, and each protein within the body has a specific function, such as antibodies, contractile proteins, enzymes, hormonal proteins, structural proteins, storage proteins and transport proteins. Determining three-dimensional structure of a protein can help researchers dis… more
Date: June 8, 2009
Creator: Zhu, Liang Cong
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Structural studies of Bcl-2-family regulators of apoptosis

Description: The Bcl-2 family of proteins includes about a dozen different proteins which share two small regions of amino acid homology but otherwise exhibit rather modest sequence similarities. The members of this family function as molecular regulators of apoptosis, some as accelerators of cell death and others as inhibitors of apoptosis. The authors analyzed the predicted secondary structures of Bcl-2-family proteins and found that a series of four amphipathic helices, three short {beta}-strands, and a … more
Date: June 1, 1996
Creator: Stevens, P. W.; Cai, X. & Schiffer, M.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

A novel family of small proteins that affect plant development

Description: The DVL genes represent a new group of plant proteins that influence plant growth and development. Overexpression of DVL1, and other members of the DVL family, causes striking phenotypic changes. The DVL proteins share sequence homology in their C-terminal half. Point mutations in the C-terminal domain show it is necessary and deletion studies demonstrate the C-terminal domain is sufficient to confer the overexpression phenotypes. The phenotypes observed, and the conservation of the protein seq… more
Date: April 29, 2011
Creator: Walker, John Charles
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
open access

Protein nutrition and metabolism during early development of the chick embryo

Description: Cultures of intact early chick embryos have been used as a model system in which to study the nutrition and metabolism of proteins during early embryonic development. Previous studies have shown that these embryos require nutrient proteins for growth and development. The protein requirement was found to be specific in that at least two proteins were essential; one a transferrin (either conalbumin or yolk transferrin) and the other either ovalbumin or lipovitellin. Variations in the quantity or … more
Date: January 1, 1976
Creator: Klein, N. W.
Item Type: Refine your search to only Article
Partner: UNT Libraries Government Documents Department
open access

Development of a Targeted Protein Residue Analysis Approach in Archaeology

Description: Liquid chromatography-mass spectrometry (LC-MS) based proteomic methods have provided archaeologists with a powerful tool for the discovery and identification of proteins within artifacts. Traditionally, discovery-based methods have utilized a non-targeted full mass scan method in an attempt to identify all proteins present within a given sample. However, increased sensitivity is often needed to target specific proteins in order to test hypotheses. Proteins present within archaeological materia… more
Date: August 2017
Creator: Scott, Ashley
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

In-Situ Survival Mechanisms of U and Tc Reducing Bacteria in Contaminated Sediments

Description: Desulfovibrio desulfuricans G20 and Shewanella oneidensis MR-1 are model subsurface organisms for studying genes involving in situ radionuclide transformation and sediment survival. Our research objective for this project has been to develop a signature-tagged mutagenesis (STM) procedure and use it to identify mutants in genes of these subsurface bacteria involved in sediment survival and radionuclide reduction. The mutant genes identified in these studies allow us for the first time to describ… more
Date: June 1, 2005
Creator: Krumholz, Lee R.
Item Type: Refine your search to only Report
Partner: UNT Libraries Government Documents Department
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