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Ultrastructural Effects of Chemical Modification on Olfactory Receptors

Description: The ultrastructural effects of chemical modification on olfactory receptors were investigated with scanning electron microscopy, transmission electron microscopy and fluorescent microscopy. Mason and Morton (1984) hypothesized that a two-step chemical treatment would covalently modify receptor proteins. Their two-step protocol was modified in an attempt to label olfactory receptor proteins and the ultra structural effects of the original two-step protocol were examined.
Date: August 1987
Creator: Thompson, Rebecca M. (Rebecca Mae)
Partner: UNT Libraries
open access

Roles of Calcium Ions and Cyclic AMP in Olfactory Transduction

Description: The roles of Ca2 + and cAMP in olfactory transduction were explored using agents which affect calcium channels and second messenger systems. These agents were applied at certain calculated final concentrations onto olfactory epithelia of urethane-anesthetized frogs (Sana PiPlens) by two-sec aerosol spray. During extracellular recording, saturated vapors of isoamyl acetate were delivered every 100 sec in 0.3 sec pulses to produce an electroolfactogram (EOG). Inorganic cations that block inward … more
Date: December 1986
Creator: Winegar, Bruce D. (Bruce David)
Partner: UNT Libraries
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