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Effect of Ribavirin on Phenotypic Reversion of Mammalian cells Transformed by Temperature-Sensitive Mutants of Avian Sarcoma Virus

Description: The effect of ribavarin (1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide), a synthetic nucleoside, on a series of cellular properties which characterize the transformed state was studied using normal rat kidney cells (NRK) and cloned derivatives transformed by a wild type avian sarcoma virus, B77-NRK, and by a temperature-sensitive mutant of the virus, LA31-NRK.
Date: December 1984
Creator: Siripont, Janya
Partner: UNT Libraries
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Carboranyl Nucleosides & Oligonucleotides for Neutron Capture Therapy Final Report

Description: This proposal enabled us to synthesize and develop boron-rich nucleosides and oligonucleotide analogues for boron neutron capture therapy (BNCT) and the treatment of various malignancies. First, we determined the relationship between structure, cellular accumulation and tissue distribution of 5-o-carboranyl-2'-deoxyuridine (D-CDU) and its derivatives D-ribo-CU and 5-o-carboranyluracil (CU), to potentially target brain and other solid tumors for neutron capture therapy. Synthesized carborane con… more
Date: December 1, 2004
Creator: Schinazi, Raymond F.
Partner: UNT Libraries Government Documents Department
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Pyrimidine Nucleoside Metabolism in Pseudomonads and Enteric Bacteria

Description: Metabolic differences in the strategies used for pyrimidine base and nucleoside salvage were studied in the pseudomonads and enteric bacteria. Fluoro--analogs were used to select mutant strains of E. coli, S. typhimurium, P. putida, and P. aeruginosa blocked in one or more of the uracil and uridine salvage enzymes. HPLC analysis of cell-free extracts from wild-type and mutant strains examined the effectiveness of the selections. Evidence was found for cytidine kinase in Pseudomonas and for an a… more
Date: December 1991
Creator: Scott, Allelia Worrall
Partner: UNT Libraries
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Evaluation Of Microdosing Strategies For Studies In Preclinical Drug Development: Demonstration Of Linear Pharmacokinetics In Dogs Of A Nucleoside Analogue Over A 50-Fold Dose Range

Description: The technique of accelerator mass spectrometry (AMS) was validated successfully and utilized to study the pharmacokinetics and disposition in dogs of a preclinical drug candidate (Compound A), after oral and intravenous administration. The primary objective of this study was to examine whether Compound A displayed linear kinetics across sub-pharmacological (microdose) and pharmacological dose ranges in an animal model, prior to initiation of a human microdose study. The AMS-derived disposition … more
Date: April 22, 2004
Creator: Sandhu, P; Vogel, J S; Rose, M J; Ubick, E A; Brunner, J E; Wallace, M A et al.
Partner: UNT Libraries Government Documents Department
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An exploration of sequence specific DNA-duplex/pyrene interactions for intercalated and surface-associated pyrene species. Final report, May 1, 1993--December 31, 1996

Description: The broad objective of this DOE sponsored work on photoinduced electron transfer (ET) within covalently modified DNA was to learn about the rates of Et among various DNA bases and commonly used organic electron donor (D) and acceptor (A) molecules. This hypothesis driven, multidisciplinary project combined skills in modified nucleic acid synthesis and in continuous and time-resolved optical spectroscopies. Covalently modified DNA chemistry as investigated in this program had two specific long t… more
Date: March 1, 1997
Creator: Netzel, T. L.
Partner: UNT Libraries Government Documents Department
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Structure of pyrR (Rv1379) from Mycobacterium tuberculosis: A persistence gene and protein drug target

Description: The 1.9 {angstrom} native structure of pyrimidine biosynthesis regulatory protein encoded by the Mycobacterium tuberculosis pyrR gene (Rv1379) is reported. Because pyrimidine biosynthesis is an essential step in the progression of TB, pyrR is an attractive antitubercular drug target. The Mycobacterium tuberculosis pyrR gene (Rv1379) encodes a protein that regulates expression of pyrimidine nucleotide biosynthesis (pyr) genes in a UMP-dependent manner. Because pyrimidine biosynthesis is an essen… more
Date: September 24, 2004
Creator: Kantardjieff, K A; Vasquez, C; Castro, P; Warfel, N M; Rho, B; Lekin, T et al.
Partner: UNT Libraries Government Documents Department
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Targeting a Novel Plasmodium falciparum Purine Recycling Pathway with Specific Immucillins

Description: Plasmodium falciparum is unable to synthesize purine bases and relies upon purine salvage and purine recycling to meet its purine needs. We report that purines formed as products of the polyamine pathway are recycled in a novel pathway in which 5'-methylthioinosine is generated by adenosine deaminase. The action of P. falciparum purine nucleoside phosphorylase is a convergent step of purine salvage, converting both 5'-methylthioinosine and inosine to hypoxanthine. We used accelerator mass spect… more
Date: May 19, 2004
Creator: Ting, L; Shi, W; Lewandowicz, A; Singh, V; Mwakingwe, A; Birck, M R et al.
Partner: UNT Libraries Government Documents Department
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Identification and preliminary characterization of acsF, a Putative Ni-insertase used in the biosynthesis of acetyl-CoA synthase from Clostridium thermoaceticum

Description: OAK-B135 The acsABCDE genes in the Clostridium thermoaceticum genome are used for autotrophic acetyl-CoA synthesis using the Wood/Ljungdahl pathway. A 2.8 kb region between acsC and acsD was cloned and sequenced. Two open reading frames, orf7 ({approx} 1.9 kb) and acsF ({approx} 0.7 kb) were identified. orf7 appears to encode an Fe-S protein, in that it contains 5 conserved cysteine residues, 3 of which are present in a motif (CXXXXXCXXC) commonly used to coordinate Fe-S clusters. However, Orf7… more
Date: January 2003
Creator: Loke, Huay-Keng & Lindahl, Paul A.
Partner: UNT Libraries Government Documents Department
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Visualization of drug-nucleic acid interactions at atomic resolution. VI. Structure of two drug/ dinucleoside monophosphate crystalline complexes, ellipticine: 5-iodocytidylyl(3'-5')guanosine and 3,5,6,8-tetramethyl-n-methyl phenanthrolinium: 5-iodocytidylyl(3'-5')guanosine

Description: Ellipticine and 3,5,6,8-tetramethyl-N-methyl phenanthrolinium (TMP) form complexes with the dinucleoside monophosphate, 5-iodocytidylyl(3'-5')guanosine (iodoCpG). These crystals are isomorphous: ellipticine-iodoCpG crystals are monoclinic, space group P2/sub 1/, with a = 13.88 A, b = 19.11 A, c = 21.42 A, ..beta.. = 105.4; TMP-iodoCpG crystals are monoclinic, space group P2/sub 1/, with a = 13.99 A, b = 19.12 A, c = 21.31 A, ..beta.. = 104.9. Both structures have been solved to atomic resolutio… more
Date: January 1, 1979
Creator: Jain, S. C.; Bhandary, K. K. & Sobell, H. M.
Partner: UNT Libraries Government Documents Department
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(Coordinated research of chemotherapeutic agents and radiopharmaceuticals)

Description: The traveler received a United Nations Development Program (UNDP) Award for Distinguished Scientists to visit Indian Research Institutions including Central Drug Research Institute (CDRI), Lucknow, the host institution, in cooperation with the Council of Scientific and Industrial Research (CSIR) of India. At CDRI, the traveler had meetings to discuss progress and future directions of on-going collaborative research work on nucleosides and had the opportunity to initiate new projects with the di… more
Date: January 14, 1991
Creator: Srivastava, P.C.
Partner: UNT Libraries Government Documents Department
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Enzymatic studies of radiation damage

Description: Progress is reported on studies related to molecular mechanisms for radiation damage to cells. Mung bean nuclease was shown to excise the A + T rich regions from different species of DNA exposed to premelting temperatures. This suggests an additional application of mung bean nuclease as a means of detection of slightly damaged reglons of DNA. A new sensitive method for the determination of nucleoside monophosphates and nucleoside 3', 5'-diphosphates was developed. The method was appli… more
Date: January 1, 1974
Creator: Laskowski, M., Sr.
Partner: UNT Libraries Government Documents Department
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Visualization of drug-nucleic acid interactions at atomic resolution. IV. Structure of an aminoacridine/dinucleoside monophosphate crystalline complex, 9-aminoacridine: 5-iodocytidylyl(3'-5')guanosine

Description: 9-Aminoacridine forms a crystalline complex with the dinucleoside monophosphate, 5-iodocytidylyl(3'-5')guanosine (iodoCpG). These crystals are monoclinic, space group P2/sub 1/, with a = 13.98 A, b = 30.58 A, c = 22.47 A and ..beta.. = 113.9. The structure has been solved to atomic resolution by Patterson and Fourier methods, and refined by a combination of Fourier and sum function Fourier methods. The asymmetric unit contains four 9-aminoacridine molecules, four iodoCpG molecules and 21 water … more
Date: January 1, 1979
Creator: Sakore, T. D.; Reddy, B. S. & Sobell, H. M.
Partner: UNT Libraries Government Documents Department
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New prodrugs based on phospholipid-nucleoside conjugates

Description: A method is described for the preparation of defined, isomerically pure phospholipid-nucleoside conjugates as a prodrug in which the drug (araC) is attached to the phospholipid by a monophosphate linkage. Key intermediates in the process involve selective blocking and deblocking of the nucleoside derivative. These particular monophosphate-linked derivatives represent a new class of prodrug, which are useful by themselves or in combination with diphosphate linked derivatives. Several new composi… more
Date: February 3, 1982
Creator: MacCoss, M.
Partner: UNT Libraries Government Documents Department
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Synthesis and evaluation of boron compounds for neutron capture therapy of malignant brain tumors. Technical progress report No. 1, May 1, 1990--January 31, 1991

Description: Boron neutron capture therapy offers the potentiality for treating brain tumors currently resistant to treatment. The success of this form of therapy is directly dependent upon the delivery of sufficient numbers of thermal-neutrons to tumor cells which possess high concentrations of B-10. The objective of this project is to develop chemical methodology to synthesize boron-containing compounds with the potential for becoming incorporated into rapidly-dividing malignant brain tumor cells and excl… more
Date: December 31, 1990
Creator: Soloway, A. H. & Barth, R. F.
Partner: UNT Libraries Government Documents Department
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An immunochemical approach to the study of DNA damage and repair. Technical progress report, May 1, 1989--April 30, 1992

Description: The overall objective of this project has been to develop immunochemical methods to quantitate unique DNA base damages in order to facilitate studies on radiation-induced damage production and repair. Specifically, we have been using antibodies raised to damaged bases to quantitate unique lesions in model systems in order to evaluate their potential biological consequences. Our approach has been to synthesize modified nucleotides or nucleosides, conjugate them to protein carriers, and use the c… more
Date: May 1, 1992
Creator: Wallace, S. S. & Erlanger, B. F.
Partner: UNT Libraries Government Documents Department
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Control points within the cell cycle

Description: Evidence of the temporal order of chromosomal DNA replication argues favorably for the view that the cell cycle is controlled by genes acting in sequence whose time of expression is determined by mitosis and the amount of nuclear DNA (2C vs 4C) in the cell. Gl and G2 appear to be carbohydrate dependent in that cells starved of either carbohydrate of phosphate fail to make these transitions. Cells deprived of nitrate, however, fail only at Gl to S transition indicating that the controls that ope… more
Date: January 1, 1984
Creator: Van't Hof, J.
Partner: UNT Libraries Government Documents Department
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